Detection of genetic resistance to cocoa black pod disease caused by three Phytophthora species

Barreto, Mariana Araújo; Santos, J. C. S.; Corrêa, Ronan Xavier; Luz, Edna Dora Martins Newman; Marelli, Jean-Philippe; Souza, Anete Pereira de

doi:10.1007/s10681-015-1490-4

Cited by 25 publications

(17 citation statements)

References 29 publications

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“…The creation of the MP01 mapping population, including the genotyping procedure and its genetic map, was described in Motamayor et al (2013), Barreto et al (2015), Royaert et al (2016), and Stack et al (2015). Briefly, this study involves 420 of the total 459 offspring from a cross between “TSH 1188” and “CCN 51.” The MP01 mapping population was planted at Mars Center for Cocoa Science in Barro Preto, Bahia, Brazil (14°42′45 N, 39°22′13 E) in the year 2000 in a 3 × 3 m grid in a hydromorphic and typical tropudalf (Itabuna modal) mixed soil type.…”

Section: Methodsmentioning

confidence: 99%

Identification of Climate and Genetic Factors That Control Fat Content and Fatty Acid Composition of Theobroma cacao L. Beans

et al. 2019

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The main ingredients of chocolate are usually cocoa powder, cocoa butter, and sugar. Both the powder and the butter are extracted from the beans of the cacao tree (Theobroma cacao L.). The cocoa butter represents the fat in the beans and possesses a unique fatty acid profile that results in chocolate’s characteristic texture and mouthfeel. Here, we used a linkage mapping population and phenotypic data of 3,292 samples from 420 progeny which led to the identification of 27 quantitative trait loci (QTLs) for fatty acid composition and six QTLs for fat content. Progeny showed extensive variation in fat levels and composition, with the level of palmitic acid negatively correlated to the sum of stearic acid, oleic acid, and linoleic acid. A major QTL explaining 24% of the relative level of palmitic acid was mapped to the distal end of chromosome 4, and those higher levels of palmitic acid were associated with the presence of a haplotype from the “TSH 1188” parent in the progeny. Within this region of chromosome 4 is the Thecc1EG017405 gene, an orthologue and isoform of the stearoyl-acyl carrier protein (ACP) desaturase (SAD) gene in plants, which is involved in fatty acid biosynthesis. Besides allelic differences, we also show that climate factors can change the fatty acid composition in the beans, including a significant positive correlation between higher temperatures and the higher level of palmitic acid. Moreover, we found a significant pollen donor effect from the variety “SIAL 70” which was associated with decreased palmitic acid levels.

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Section: Methodsmentioning

confidence: 99%

Identification of Climate and Genetic Factors That Control Fat Content and Fatty Acid Composition of Theobroma cacao L. Beans

et al. 2019

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“…The female parent is related to Iquitos and Nanay genetic groups 13,24 , and the male parent has predominant ancestries from Iquitos, Criollo, and Amelonado 13,14,24 . Those parents contrast for many important traits, and the MP01 progenies segregate for disease resistance 11,29,51 , pod color 5 , fat content and fat composition 25 . The MP01 population also segregates for yield.…”

Section: Mp01 Segregating Mapping Populationmentioning

confidence: 99%

QTL mapping and identification of SNP-haplotypes affecting yield components of Theobroma cacao L.

Fernandes¹,

Corrêa

Ingram³

et al. 2020

Hortic Res

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Cacao is a crop of global relevance that faces constant demands for improved bean yield. However, little is known about the genomic regions controlling the crop yield and genes involved in cacao bean filling. Hence, to identify the quantitative trait loci (QTL) associated with cacao yield and bean filling, we performed a QTL mapping in a segregating mapping population comprising 459 trees of a cross between 'TSH 1188' and 'CCN 51'. All variables showed considerable phenotypic variation and had moderate to high heritability values. We identified 24 QTLs using a genetic linkage map that contains 3526 single nucleotide polymorphism (SNP) markers. Haplotype analysis at the significant QTL region on chromosome IV pointed to the alleles from the maternal parent, 'TSH 1188', as the ones that affect the cacao yield components the most. The recombination events identified within these QTL regions allowed us to identify candidate genes that may take part in the different steps of pod growth and bean filling. Such candidate genes seem to play a significant role in the source-to-sink transport of sugars and amino acids, and lipid metabolism, such as fatty acid production. The SNP markers mapped in our study are now being used to select potential highyielding cacao varieties through marker-assisted selection in our existing cacao-breeding experiments.

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“…Cacao trees are constantly confronted with important pathogens that target vegetative shoots, pods and flower cushions ( Moniliophthora perniciosa ) (Aime and Phillips-Mora, 2005 ), pods and leaves ( Phytophthora spp.) (Barreto et al, 2015 ; Surujdeo-Maharaj et al, 2016 ), and the vascular system ( Ceratocystis cacaofunesta ) (Engelbrecht and Harrington, 2005 ). Of these diseases, Ceratocystis wilt of cacao (CWC) is one the most important, mainly because it causes plant death.…”

Section: Introductionmentioning

confidence: 99%

“…An F1 mapping population (MP01) created from crosses between two different parents that segregate for CWC has been used to identify QTLs associated with several traits, for instance pod color regulation (Motamayor et al, 2013 ), witches' broom (Silva et al, 2014 ; Royaert et al, 2016 ) and black pod resistance (Bahia et al, 2015 ; Barreto et al, 2015 ). Despite CWC being a lethal disease, few studies have focused on discovering molecular markers associated with resistance genes.…”

Section: Introductionmentioning

confidence: 99%

Mapping of a Major QTL for Ceratocystis Wilt Disease in an F1 Population of Theobroma cacao

Fernandes¹,

Royaert

Corrêa

et al. 2018

Front. Plant Sci.

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Cacao is an important crop, its beans are key raw materials for the chocolate and cosmetic industries. Ceratocystis wilt of cacao (CWC) caused by Ceratocystis cacaofunesta is a lethal disease for the crop. Therefore, the selection of resistant cacao varieties is one of the viable ways to minimize losses in cacao production. In this paper, we described the identification of a major QTL associated with CWC in an F1 mapping population from a cross between a resistant, “TSH 1188,” and a susceptible genotype, “CCN 51.” A set of 266 trees were genotyped using 3,526 single nucleotide polymorphic markers and then multiple QTL mapping analyses were performed. Two QTLs were identified on chromosomes IV and VI. The major QTL was located at 20 cM from the top position of chromosome VI, accounting for more than 60% of the phenotypic variation. The favorable allele T1, with haplotype GTT, came from the “TSH 1188” parent. It was evident that the haplotype combination T1C2 on chromosome VI was the most significant for resistance, since 93% of resistant trees had this haplotype. The major QTL converged to a genomic region of 739.4 kb that harbored nine candidate genes, including two major classes of resistance genes, which would make them the primary candidates involved in the resistance to CWC. The haplotypes detected are now used to improve the efficiency and precision of the selection of resistant trees in cacao breeding.

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Detection of genetic resistance to cocoa black pod disease caused by three Phytophthora species

Cited by 25 publications

References 29 publications

Identification of Climate and Genetic Factors That Control Fat Content and Fatty Acid Composition of Theobroma cacao L. Beans

Identification of Climate and Genetic Factors That Control Fat Content and Fatty Acid Composition of Theobroma cacao L. Beans

QTL mapping and identification of SNP-haplotypes affecting yield components of Theobroma cacao L.

Mapping of a Major QTL for Ceratocystis Wilt Disease in an F1 Population of Theobroma cacao

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