Detection of DNA damage in fish Oreochromis mossambicus induced by co-exposure to phenanthrene and nitrite by ESI-MS/MS

Wahidulla, Solimabi; Rajamanickam, Yeziel Rani

doi:10.1007/s11356-009-0198-1

Cited by 19 publications

(9 citation statements)

References 35 publications

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“…In fact, electrospray ionization tandem mass spectrometric (ESI-MS/MS) analysis was capable of revealing DNA adducts in different aquatic organisms (e.g. fish (Oreochromis mossambicus) and mussel (Perna perna) soft tissue) [185,186]. This MS/MS approach provided a rapid determination and discrimination of structurally different phenanthrene derived DNA adducts in fish bile samples [186].…”

Section: Dna Adducts From Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

“…fish (Oreochromis mossambicus) and mussel (Perna perna) soft tissue) [185,186]. This MS/MS approach provided a rapid determination and discrimination of structurally different phenanthrene derived DNA adducts in fish bile samples [186]. This technique is able to detect one modified base in 10 6 -10 12 unmodified bases [187].…”

Section: Dna Adducts From Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

See 1 more Smart Citation

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

Pampanin¹,

Sydnes²

2013

Hydrocarbon

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Section: Dna Adducts From Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Section: Dna Adducts From Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

Pampanin¹,

Sydnes²

2013

Hydrocarbon

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“…Estas dosis fueron seleccionadas de acuerdo a estudios previos con fenantreno los cuales reportan en otras especies de peces el uso de dosis desde 1 hasta 20 µg/g PV (Pathiratne y Hemachandra, 2010;Wahidulla y Rajamanickam, 2010;Whyte et al, 2000).…”

Section: Diseño Experimentalunclassified

“…A este respecto, se han realizado estudios de exposición a PHE en peces teleósteos, usando la vía intraperitoneal (IP) con dosis variadas (1, 5, 20 mg/kg PV) en especies como tilapia (Oreochromis niloticus), carpa común (Cyprinus carpio), bagre del canal (Ictalurus punctatus), fundulo (Fundulus heteroclitus) y tilapia negra (Oreochromis mossambicus) (Pathiratne y Hemachandra, 2010;Wahidulla y Rajamanickam, 2010;Whyte et al, 2000), utilizando aceite vegetal como vehículo (Shailaja et al, 2006).…”

Section: Introductionunclassified

Inducción de micronúcleos y otras anormalidades nucleares en Astyanax gr. bimaculatus (Pisces: Characidae) expuestas a fenantreno

et al. 2012

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Inducción de micronúcleos y otras anormalidades nucleares en Astyanax gr. bimaculatus (Pisces: Characidae)... 237 ARTÍCULO ORIGINAL/ORIGINAL ARTICLE ResumenEl fenantreno es un hidrocarburo policíclico aromático (HPA) asociado a la actividad petrolera, altamente lipofílico, con amplia distribución y con la propiedad de persistir en el ambiente por tiempos prolongados. A pesar de su impacto, el efecto de los HPA ha sido poco estudiado en peces tropicales; por tanto, el objetivo de este estudio fue determinar la presencia de micronúcleos y otras anormalidades nucleares en eritrocitos de sangre periférica de Astyanax gr. bimaculatus expuestas a fenantreno. Los peces (n=9) fueron inyectados intraperitonealmente con 0.1, 1 y 10 µg/g de PV de fenantreno (PHE, Sigma Aldrich, pureza 98%) disuelto en aceite vegetal, un control positivo inyectados con ciclofosfamida y un control negativo con aceite vegetal. Se colectaron muestras de sangre periférica a las 0 y 96 horas de la inyección. A las 96 horas, las dosis más altas de fenantreno mostraron efectos genotóxicos sobre los eritrocitos de Astyanax gr. bimaculatus, evidenciados por la formación de micronúcleos y alteraciones morfológicas nucleares. Estos resultados sugieren que Astyanax gr. bimaculatus, especie íctica dulceacuícola nativa, tiene gran potencial para ser utilizada como bioindicador de genotoxicidad causada por hidrocarburos policíclicos aromáticos.

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“…Perhaps the sensitivity of different fish species to the toxic effects of nitrite is due to physiological characteristics of the regulatory mechanisms of ion homeostasis, determining levels of income and accumulation of exogenous nitrite in fish (Jensen 2007). Along with this, some authors suggest that nitrite, which have high reactivity in aqueous media may be involved in the formation of complex compounds with other xenobiotics-polycyclic aromatic hydrocarbons (Wahidulla and Rajamanickam 2010). Studies conducted by the authors quoted a source, demonstrating that the reaction products nitrosation polycyclic aromatic hydrocarbons can vary extremely high cytotoxicity.…”

Section: Introductionmentioning

confidence: 99%

Features of nitrogen metabolism in fishes

Dolomatov

Shekk

Zukow

et al. 2011

Rev Fish Biol Fisheries

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Presence in water is easily metabolized nitrogen-containing substances is a vital condition for the existence of food organisms in ponds used for fish reproduction. At the same time, the end products of nitrogen metabolism-ammonia, nitrites and nitrates are always displayed the body of fish into the environment, adding to the total number of these compounds dissolved in water. Ammonia, nitrite and nitrate anions are formed during biological processes, as well as man-made origin, have toxic properties and are considered as environmental factors that may limit fish productivity of reservoirs. The results of a brief review of the literature showed that the features of nitrogen metabolism in fish are actual direction of research, both theoretical science and in practical fish farming. Published the results of studies of amino acid metabolism in fish suggest that, firstly, the processes responsible for the neutralization and the release of ammonia from the body of fish, as well as ontogenetic aspects of the dynamics of formation and emission of ammonia is not fully understood. Meanwhile, several authors stressed the importance of this area of interest for the formation of science-based ways to improve the efficiency of plant breeding in fish culture. Secondly, endogenous nitrite, being the product of oxidation of the molecule nitric oxide and its transport form, the norm are always present in fish. However, the mechanisms responsible for maintaining the stability of the concentration of nitrite in the internal environment of fish, not well understood. Also, in our opinion, of interest to determine the physiological rules of nitrites and nitrates in the body of various species, depending on the seasons and stages of ontogeny. Thirdly, the literature pays much attention to the toxic properties of nitrite in water biocenosis. Certainly, the analysis of the manifestation in fish tissue-specific toxic effects of nitrites and their derivatives-an important area of research. Nevertheless, we can not exclude that it may be quite promising analysis of the incorporation of exogenous nitrite as a substrate exist in fish of the physiological mechanisms re-synthesis of nitric oxide. This kind of ''interference'' of endogenous and exogenous nitrite-anions are not causing serious structural damage to organs and tissues, however, can cause substantial damage to the practical fish farming, causing changes in the level of regulatory mechanisms of fish at the most critical stages: when working with manufacturers, the incubation of eggs, and larvae rearing and wintering fish.

show abstract

Detection of DNA damage in fish Oreochromis mossambicus induced by co-exposure to phenanthrene and nitrite by ESI-MS/MS

Cited by 19 publications

References 35 publications

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

Inducción de micronúcleos y otras anormalidades nucleares en Astyanax gr. bimaculatus (Pisces: Characidae) expuestas a fenantreno

Features of nitrogen metabolism in fishes

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