Detection of clonal T-cell receptor gamma gene rearrangements with the use of the polymerase chain reaction in cutaneous lesions of mycosis fungoides and Sezary syndrome

Bachelez, Hervé

doi:10.1001/archderm.131.9.1027

Cited by 36 publications

(49 citation statements)

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“…DNA cytophotometry, 33-37 nuclear morphometry, 38-41 immunohistochemistry, 42-57 chromosomal studies, [58][59][60] and, more recently, molecular genetic analysis of T-cell clonality 8,[55][56][57][61][62][63][64][65][66][67][68][69][70][71][72][73][74][75][76][77][78] have generated a significant amount of data that suggest their utility not only in the early diagnosis of MF but also potentially in the clinical management of MF patients. How these data may be integrated with light microscopy to aid in the definitive diagnosis of CTCL has not been standardized.…”

Section: Early Diagnosis Of Mf: the Role Of Ancillary Techniquesmentioning

confidence: 99%

Defining early mycosis fungoides

Pimpinelli¹,

Olsen

Santucci³

et al. 2005

Journal of the American Academy of Dermatology

466

389

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This editorial review summarizes the results of 5 meetings sponsored by the International Society for Cutaneous Lymphoma at which the clinicopathologic and ancillary features of early mycosis fungoides were critically examined. Based on this analysis, an algorithm was developed for the diagnosis of early mycosis fungoides involving a holistic integration of clinical, histopathologic, immunopathologic, and molecular biological characteristics. A novel aspect of this algorithm is that it relies on multiple types of criteria rather than just one, for example, histopathology. Before its finalization, the proposed diagnostic algorithm will require validation and possibly further refinement at multiple centers during the next several years. It is anticipated that a more standardized approach to the diagnosis of early mycosis fungoides will have a beneficial impact on the epidemiology, prognostication, treatment, and analysis of clinical trials pertaining to this most common type of cutaneous lymphoma.

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Section: Early Diagnosis Of Mf: the Role Of Ancillary Techniquesmentioning

confidence: 99%

Defining early mycosis fungoides

Pimpinelli¹,

Olsen

Santucci³

et al. 2005

Journal of the American Academy of Dermatology

466

389

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“…30 However, such results are similar to those of the literature especially for MF. 47 Regarding the pleomorphic CTCL, a clonal TCR positivity was found in only two cases of six but, to our knowledge, very few molecular data have been published on this rare form of CTCL. [47][48][49] Our series emphasizes the need for a comparison of clinical, histopathological, hematological, virological and molecular studies to make a proper diagnosis of CTCL, especially since in tropical areas, differential diagnosis between MF and SS on one hand and smoldering ATL on the other hand is sometimes very difficult.…”

Section: Discussionmentioning

confidence: 84%

“…[46][47][48][49] Clonal TCR␥ gene rearrangement was detectable in the skin and/or the PBMC in 1/1 ATL, 3/3 SS, 1/2 MF, and strikingly only 2/6 pleomorphic CTCL (including one indeterminate MF/pCTCL). This may be due to partial infiltration by the malignant cells in some of the biopsies used for DNA extraction, since the PCR technique for TCR detection has a sensitivity of approximately 5%.…”

Section: Discussionmentioning

confidence: 99%

Cutaneous T cell lymphomas: mycosis fungoides, Sezary syndrome and HTLV-I-associated adult T cell leukemia (ATL) in Mali, West Africa: a clinical, pathological and immunovirological study of 14 cases and a review of the African ATL cases

et al. 1998

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Cutaneous T cell lymphomas (CTCL) are rare lymphoproliferative diseases, which are frequently suspected to be of viral origin. As very few data were available concerning cutaneous T cell lymphomas in tropical Africa, we undertook a clinical, histopathological, immunological and viro-molecular study of patients with a clinical diagnosis of cutaneous lymphoma, in Bamako, Mali. While prior to this study, no case of CTCL had been reported in this country, 14 patients (five women, nine men; mean age 58 years) with a diagnosis of cutaneous lymphoma were seen over a period of 30 months (1992-1994) in the only dermatological department in Mali. Clinically, the most frequent pattern was an infiltrated erythrodermia similar to Sezary syndrome. Nodular lesions and/or plaques were rarely observed. All these cutaneous tumors were T cell lymphoproliferations, only one expressing the CD8 + antigen. A comprehensive analysis of all the available data permitted characterization of three cases of adult T cell leukemia/lymphoma (ATL) associated with HTLV-I (one definitive case, of leukemic type, with demonstration of clonal integration of HTLV-I proviral genome and two probable ATL cases), three cases of Sezary syndrome (SS), two cases of mycosis fungoides (MF) and five cases of pleomorphic cutaneous lymphoma. In one case, the differentiation between MF and pleomorphic cutaneous lymphoma could not be established. HTLV-I serological and/or molecular markers were restricted to the three ATL cases. From the unique definitive ATL case, a T cell line was established from culture of peripheral blood mononuclear cells and sequence analysis of the env gene and the U3-LTR region demonstrated that the virus present in this patient belonged to the cosmopolitan subtype A. Thus, we report here the first evidence of HTLV-I infection and associated ATL in Mali. This is the second ATL case described for the whole Sahelian region (one ATL of the lymphoma type was reported previously in a Mauritanian patient). Furthermore, we demonstrate that the main types of CTCL described in Europe and North America are also present in this African area and that the prevalence of these diseases is greatly underestimated in such regions. Furthermore, no association was observed between HTLV-I/II infection and SS, MF or pleomorphic cutaneous lymphoma in Mali in contrast to other studies.

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“…Also, this can be a false-negative result, due to poor sampling, i.e. taking the skin sample with small number of malignant T-cells, and there is a possibility that primers used in this study did not cover all possible TCR-g gene rearrangements (5,21,22).…”

Section: Patients With Idiopathic Erythroderma and Clinical Suspicionmentioning

confidence: 99%

“…In 1 patient with erythrodermic mycosis fungoides, a dominant clone was not detected. In the late stages of the disease, there is a possibility of TCR gene deletion only during malignant transformation (21). Also, this can be a false-negative result, due to poor sampling, i.e.…”

Section: Patients With Idiopathic Erythroderma and Clinical Suspicionmentioning

confidence: 99%

T-Cell Receptor-G Gene Rearrangement Analysis in the Diagnosis of Patients with Erythroderma / Dijagnostički Značaj Analize Preuređenja Gena Gama Lanca T-Ćelijskog Receptora Kod Pacijenata Sa Eritrodermijom

Kandolf-Sekulović¹,

Cikota²,

Dinić³

et al. 2009

Serbian Journal of Dermatology and Venerology

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The diagnosis of erythroderma is challenging, since clinical, histopathological and immunophenotypic findings are insufficient to differentiate between inflammatory and lymphomatous erythroderma. Thus, multiplex PCR was used for T-cell receptor-g gene rearrangement analysis, in the skin and peripheral blood samples of 24 patients (20 men and 4 women) with erythroderma of varying origin, in order to estimate its diagnostic value. Cutaneous T-cell lymphoma was confirmed in 9, benign inflammatory dermatosis in 12, and idiopathic erythroderma and clonal dermatitis in 3 patients. In the group of patients with erythrodermic cutaneous T-cell lymphoma, the dominant clone was detected in the skin of 8/9, and in none of the patients with inflammatory dermatoses. A dominant clone was found in peripheral blood of 5/6 samples of patients with erythrodermic cutaneous T-cell lymphoma, and in 2/12 patients with inflammatory dermatosis. T-cell receptor-g gene rearrangement analysis is valuable in differentiation between inflammatory and lymphomatous erythroderma, thus substantially improving the diagnosis of patients with erythroderma.

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Detection of clonal T-cell receptor gamma gene rearrangements with the use of the polymerase chain reaction in cutaneous lesions of mycosis fungoides and Sezary syndrome

Cited by 36 publications

References 0 publications

Defining early mycosis fungoides

Defining early mycosis fungoides

Cutaneous T cell lymphomas: mycosis fungoides, Sezary syndrome and HTLV-I-associated adult T cell leukemia (ATL) in Mali, West Africa: a clinical, pathological and immunovirological study of 14 cases and a review of the African ATL cases

T-Cell Receptor-G Gene Rearrangement Analysis in the Diagnosis of Patients with Erythroderma / Dijagnostički Značaj Analize Preuređenja Gena Gama Lanca T-Ćelijskog Receptora Kod Pacijenata Sa Eritrodermijom

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