Hans J. Nelis, Luc De Vuyst, Karen Goubert, Bart V. Devreese, Jozef J. Van Beeumen, Jos G. Raymackers, Erick J. Vandamme, AndréP. De Leenheer
Abstract:As part of ongoing studies in the biosynthesis of the lantibiotic nisin, we investigated the proteolytic cleavage of a synthetic Fmoc-labeled decapeptide mimicking a key amino acid sequence of the precursor prenisin in extracts of a Lactococcus lactis subsp. lactis strain. Reverse-phase high-performance liquid chromatography with photodiode array detection was used to trace and purify potential enzymatic conversion products. Of the three newly appearing chromatographic peaks, one was identified by means of ele…
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