Detection of chromosomal blaCTX-M-15 in Escherichia coli O25b-B2-ST131 isolates from the Kinki region of Japan

Hirai, Itaru; Fukui, Naoki; Taguchi, Masumi; Yamauchi, Kou; Nakamura, Tatsuya; Okano, Sho; Yamamoto, Yoshimasa

doi:10.1016/j.ijantimicag.2013.08.005

Cited by 41 publications

(27 citation statements)

References 29 publications

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“…The K. pneumoniae isolate belonged to ST392, previously associated with the dissemination of bla KPC , bla , and other ESBLs (13,14). In addition, this isolate also harbored a chromosomal bla and bla CTX-M-15 , the latter located downstream of ISEcp1, as previously reported in other Enterobacteriaceae isolates from Spain, Japan, Germany, the Netherlands, and the United Kingdom (15)(16)(17)(18) 15 , and bla OXA-1 ). In this case, the second copy of bla CTX-M-15 was found to be part of a previously reported structure: a Tn3-like transposon also carrying bla TEM-1 which has its tnpA gene disrupted by ISEcp1-bla CTX-M-15 (19).…”

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confidence: 57%

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Rojas

Wright

Cadena

et al. 2016

Antimicrob Agents Chemother

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fWe report complete genome sequences of four bla NDM-1 -harboring Gram-negative multidrug-resistant (MDR) isolates from Colombia. The bla NDM-1 genes were located on 193-kb Inc FIA, 178-kb Inc A/C2, and 47-kb (unknown Inc type) plasmids. Multilocus sequence typing (MLST) revealed that these isolates belong to sequence type 10 (ST10) (Escherichia coli), ST392 (Klebsiella pneumoniae), and ST322 and ST464 (Acinetobacter baumannii and Acinetobacter nosocomialis, respectively). Our analysis identified that the Inc A/C2 plasmid in E. coli contained a novel complex transposon (Tn125 and Tn5393 with three copies of bla NDM-1 ) and a recombination "hot spot" for the acquisition of new resistance determinants.A t this time, bla NDM is recognized as a major global health threat. Guatemala and Colombia reported the first cases of bla NDM-1 -harboring isolates in Latin America (1, 2). In both instances, bla NDM-1 was discovered in hospital-acquired, clonally related Klebsiella pneumoniae isolates that were recovered from patients who had not travelled recently. The molecular epidemiology of bla NDM carbapenemases in South America has been investigated only in a limited fashion, and data in Colombia are very scarce. In order to understand the dissemination of bla NDM-1 in Colombia, we performed a genomic analysis of four sentinel isolates, Acinetobacter baumannii, Acinetobacter nosocomialis, Escherichia coli, and K. pneumoniae collected in 2012, shortly after the first reported outbreak (1).

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supporting

confidence: 57%

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Rojas

Wright

Cadena

et al. 2016

Antimicrob Agents Chemother

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“…The bla CTX-M-2 gene is the most prevalent ESBL-encoding gene in human E. coli isolates in Brazil [14] and is frequently reported in animals and environmental sources [1]. Although bla CTX-M genes are usually harboured in plasmids, the chromosomal insertion of these genes was found in E. coli [15][16][17]. Herein, we showed the chromosomal bla CTX-M-2 in diverse E. coli isolates.…”

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confidence: 78%

Detection of chromosomal blaCTX-M-2 in diverse Escherichia coli isolates from healthy broiler chickens

Ferreira

Filho

Andrade

et al. 2014

Clinical Microbiology and Infection

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The rise of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli in food-producing animals is a growing concern for public health. We investigated ESBL producers isolated from broiler chickens in Brazil and characterized 19 CTX-M-2-producing E. coli. The ISCR1 was detected upstream of the chromosome-located gene bla(CTX-M-2), associated with sul-1 type integron structure. CTX-M-2-producing E. coli exhibited different PFGE-types and phylogenetic groups, showing a non-clonal dissemination. The sequence types found (ST93, ST155 and ST2309) have been associated with humans and animals worldwide. Herein, we report the chromosomal location of bla(CTX-M-2) on E. coli, highlighting the risks of multidrug-resistant bacteria in food-producing animals.

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“…However, one or several copies of the bla CTX-M-15 gene have been detected on the chromosome (9,(115)(116)(117). Some strains with chromosomally encoded CTX-M-15 harbor an IncFII-type plasmid, but without the bla CTX-M-15 gene.…”

Section: Antibiotic Resistancementioning

confidence: 99%

Escherichia coli ST131, an Intriguing Clonal Group

2014

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SUMMARY In 2008, a previously unknown Escherichia coli clonal group, sequence type 131 (ST131), was identified on three continents. Today, ST131 is the predominant E. coli lineage among extraintestinal pathogenic E. coli (ExPEC) isolates worldwide. Retrospective studies have suggested that it may originally have risen to prominence as early as 2003. Unlike other classical group B2 ExPEC isolates, ST131 isolates are commonly reported to produce extended-spectrum β-lactamases, such as CTX-M-15, and almost all are resistant to fluoroquinolones. Moreover, ST131 E. coli isolates are considered to be truly pathogenic, due to the spectrum of infections they cause in both community and hospital settings and the large number of virulence-associated genes they contain. ST131 isolates therefore seem to contradict the widely held view that high levels of antimicrobial resistance are necessarily associated with a fitness cost leading to a decrease in pathogenesis. Six years after the first description of E. coli ST131, this review outlines the principal traits of ST131 clonal group isolates, based on the growing body of published data, and highlights what is currently known and what we need to find out to provide public health authorities with better information to help combat ST131.

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Detection of chromosomal blaCTX-M-15 in Escherichia coli O25b-B2-ST131 isolates from the Kinki region of Japan

Cited by 41 publications

References 29 publications

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Detection of chromosomal blaCTX-M-2 in diverse Escherichia coli isolates from healthy broiler chickens

Escherichia coli ST131, an Intriguing Clonal Group

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Detection of chromosomal blaCTX-M-15 in Escherichia coli O25b-B2-ST131 isolates from the Kinki region of Japan

Cited by 41 publications

References 29 publications

Initial Assessment of the Molecular Epidemiology of bla NDM-1 in Colombia

Initial Assessment of the Molecular Epidemiology of bla NDM-1 in Colombia

Detection of chromosomal blaCTX-M-2 in diverse Escherichia coli isolates from healthy broiler chickens

Escherichia coli ST131, an Intriguing Clonal Group

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Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia

Initial Assessment of the Molecular Epidemiology of bla _NDM-1 in Colombia