Detection of Chromosomal Aberrations in Prostate Cancer by Fluorescence In Situ Hybridization (FISH)

Celep, Figen; Karagüzel, Ahmet; Özgür, Güner Kemal; Yildiz, Kadriye

doi:10.1016/s0302-2838(03)00414-7

Cited by 8 publications

(9 citation statements)

References 33 publications

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“…Aneusomy of chromosome 7 was reported to be associated with higher histological grade, advanced pathological stage, and early PC death in other studies (12,14,(30)(31)(32). Trisomy 7 was described as a common anomaly in prostate carcinoma (22,(33)(34)(35) and was associated with aggressive tumor behavior and poor prognosis (13,15,36). There was statistical difference in the aneusomies of chromosome 7 between the Japanese (15) and Singaporean cases with respect to pT2 pathological stage and GS !…”

Section: Discussionmentioning

confidence: 85%

“…Aneusomies of chromosome 7 have also been observed frequently with the gain of chromosome 7 and loss of 7q31 as the most common karyotypic anomalies (12). Recently, monosomy 7 was also observed in Turkish patients using fluorescence in situ hybridization (FISH) (13). Chromosome 7 alterations were known to be associated with higher tumor grade, advanced pathological stage, and poor prognosis (12,14,15).…”

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confidence: 99%

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Chromosomal changes in prostate cancer: A fluorescence in situ hybridization study

Das¹,

Lau²,

Sivaswaren³

et al. 2005

Clinical Genetics

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The incidence of prostate cancer (PC) is increasing steadily with the aging population in Singapore. As the pattern of chromosomal aberrations in Asian men with PC is poorly understood, we investigated the numerical aberrations for chromosomes 7, 8, 11, and 17 by fluorescence in situ hybridization (FISH). FISH was performed on standard sections and tissue microarrays of 54 PC and 33 benign prostatic hyperplasia (BPH) specimens. Among the 54 PC specimens, FISH detected 44 cases as aneusomy and two as disomy and was unsuccessful for eight cases. Cytogenetic alterations of two or more chromosomes per tumor were detected in 33/46 (72%) PCs. The most frequent alteration was aneusomy of chromosome 8 detected in 34/46 (74%) cases followed by numerical aberrations in chromosome 7 (61%). Gain of 8q24, loss of chromosome 7, and gain of 11q13 were associated with higher Gleason score and were statistically significant. Gain of chromosome 7 was more common in locally advanced disease, while gain of chromosome 11q13 and chromosome 7 was more common in metastatic disease.

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Section: Discussionmentioning

confidence: 85%

mentioning

confidence: 99%

Chromosomal changes in prostate cancer: A fluorescence in situ hybridization study

Das¹,

Lau²,

Sivaswaren³

et al. 2005

Clinical Genetics

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“…The total percentage of nuclei containing 1, 2, 3, 4, and z4 signals was determined for each chromosome using the criteria reported by Celep et al (25).…”

Section: Methodsmentioning

confidence: 99%

“…We increased the cutoff value for tetrasomy and hypertetrasomy to 1% for all chromosomes to avoid misinterpretation. After defining the cutoff values, the following criteria were used to classify the tumor samples: (a) an abnormal monosomy, trisomy, tetrasomy, or hypertetrasomy required a percentage of nuclei showing one, three, four, and more than four signals greater than or equal to the defined cutoff level; (b) a tumor was classified as aneuploid if at least one abnormal monosomy or trisomy or tetrasomy was found at one or more chromosomes; (c) a tumor was classified as triploid (tetraploid) if the autosomal average of the percentage of nuclei with three (four) signals was z10% (25,26).…”

Section: Methodsmentioning

confidence: 99%

Allelotypes and Fluorescence In situ Hybridization Profiles of Poorly Differentiated Endocrine Carcinomas of Different Sites

Furlan

Bernasconi

Uccella

et al. 2005

Clinical Cancer Research

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Purpose: The aim of this work was to investigate the genotypic profiles of 36 poorly differentiated endocrine carcinoma (PDEC) of different sites to verify if their very similar phenotype may reflect similar pattern of genetic anomalies and if useful diagnostic or prognostic markers may be pointed out. Experimental Design: All tumors were microallelotyped at 57 microsatellite on 11 autosomes and the allelotypes of a selected panel of tumors were validated by interphasic fluorescence in situ hybridization with centromeric probes for chromosomes 1, 3, 6, 11, 17, and 18 and a probe specific for p53. Results: Regardless of the primary sites, PDECs exhibit very complex allelotypes (86%) and TP53 allelic imbalance (89%). Among these cases, fluorescence in situ hybridization analysis confirmed the presence of multiple aneusomies and a chromosome instability phenotype. Very low percentage of allelic imbalance (AI) and few aneuploidies were detected in only five PDECs for which an overall longer survival was observed. We found recurrent AI on 3p, 5, and 11q13 in lung PDECs, on 5q21, 8p, and 18q21 in colorectal PDECs and on 7 and 11q22 in gastric PDECs. Significantly better outcome was observed in patients with PDEC exhibiting 8q AIs and absence of AI at chromosome regions 6q25 and 6p. Conclusions: The concurrence of p53 inactivation and aneuploidies or chromosome instability are the main features of PDECs. However, the specific allelotypes observed in relation to primary site support the hypothesis that PDECs and exocrine carcinomas of all sites may share early pathogenetic mechanisms. Molecular markers of potential diagnostic and prognostic values for PDECs of different sites have been identified.

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“…Frequency and relevance of Y losses in prostate cancer are unclear. Studies using FISH have described Y losses in 14–53% of prostate cancers 20–26. One group described a particularly high frequency of Y losses (89%) in 12 hormone refractory prostate carcinomas analyzed by karyotyping 27.…”

Section: Introductionmentioning

confidence: 99%

Y chromosome losses are exceedingly rare in prostate cancer and unrelated to patient age

Stahl¹,

Kilgué²,

Tennstedt³

et al. 2011

The Prostate

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Detection of Chromosomal Aberrations in Prostate Cancer by Fluorescence In Situ Hybridization (FISH)

Cited by 8 publications

References 33 publications

Chromosomal changes in prostate cancer: A fluorescence in situ hybridization study

Chromosomal changes in prostate cancer: A fluorescence in situ hybridization study

Allelotypes and Fluorescence In situ Hybridization Profiles of Poorly Differentiated Endocrine Carcinomas of Different Sites

Y chromosome losses are exceedingly rare in prostate cancer and unrelated to patient age

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