Acta Biotechnol. volume 18, issue 3, P231-240 1998 DOI: 10.1002/abio.370180306 View full text
S. Kleinsteuber, D. Hoffmann, R. H. Müller, W. Babel

Abstract: In various bacterial strains belonging to the Psubdivision of proteobacteria which are capable of degrading chlorinated monoaromatic compounds, chlorocatechol 1,2-dioxygenase genes were detected by PCR and Southern hybridization. Using PCR primers derived from the conserved sequence motifs of chlorocatechol 1,2-dioxygenase genes fdC, clcA and rcbC. PCR products of the expected size were obtained with the test strains, but not with negative control strains. The specificity of the PCR products was verified by h…

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