Detection of bovine rotavirus in diarrheic calves by using rapid test in some Mid-Euphrates provinces

Hassan, Hahmed Abass; Kshash, Qassim Haleem; Mansour, Kalefa Ali

doi:10.29079/vol13iss2art297

Cited by 4 publications

(5 citation statements)

References 20 publications

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“…The present results demonstrated (38%) and (30%) of Rotavirus infection in Karbala and Basrah provinces respectively (Table 3). This result was compatible with study of (19) found there is no significant variances between Male and female (37.5%, 40% respectively) were seen diseased. While (20) found range prevalence or Rotavirus A by using chromatographic immunoassay (Monoclonal antibodies) to capture the attendance of rotavirus virus antigen with 71% (85 of 119 samples) were highest positive in Wasit province, but the lowest positive was in Dyala province 15% (4 of 26 samples) in middle provinces regions of Iraq from cattle and buffalo calf with acute diarrhea.…”

Section: Rotavirus Prevalence In Bovine Fecal Samples By Imunochromotsupporting

confidence: 92%

Epidemiological and molecular study of Rotavirus infection among human and animal in Karbala and Basrah provinces

Aldawmy¹,

Thwiny²,

Almaali³

2021

IJVS

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This study was aimed to determine the prevalence of rotaviruses in Karbala and Basrah provinces, the genetic reassortment of human and animal rotavirus strains and the novel strains. Rotaviruses were detected by Immunochromatography Test (ICT) then the positive samples were tested by reverse transcription PCR (RT-PCR) using specific primers to VP7 gene, this gene is responsible for VP7 antigen which is responsible for stimulation the immune system to produce neutralizing antibodies. The VP7 gene was implemented in rotavirus vaccine of Iraqi immunization program. The RT PCR results showed that 56.3% (27/48) of samples positive in children under five years of age in Karbala province while Basrah province revealed 58.5% (31/53) positive samples in children whereas samples taken from calves revealed 43.1% (22/51) and 45.5% (25/55) positive samples in Karbala and Basrah provinces respectively. The sequencing of human and animal samples revealed that there was genetic reassortment between human and animal strains while in comparing with international strains there was closely related with Indian and Pakistani human strains. Necessarily for further bioinformatics studies are needed to study the genetic alterations with viral proteins analysis of rotavirus.

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Section: Rotavirus Prevalence In Bovine Fecal Samples By Imunochromotsupporting

confidence: 92%

Epidemiological and molecular study of Rotavirus infection among human and animal in Karbala and Basrah provinces

Aldawmy¹,

Thwiny²,

Almaali³

2021

IJVS

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“…Remarkably, the prevalence of this viral infection was found to be much higher in other countries compared to our study, e.g. 63% in Argentina [4], 79.9% in Australia [33], 32.5% in Sudan [34] and 36% in Iraq [35]. Different spatial, temporal and management-related factors might influence the prevalence of BRV infection.…”

Section: Epidemiology Of Bovine Rotaviruscontrasting

confidence: 56%

Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh

et al. 2022

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Bovine rotavirus (BRV) is considered the leading cause of calf diarrhea worldwide, including Bangladesh. In this study we aimed to identify risk factors for BRV infection and determine the G and P genotypes of BRV strains in diarrheic calves. Fecal samples were collected from 200 diarrheic calves in three districts between January 2014 and October 2015. These samples were screened to detect the presence of BRV using rapid test-strips BIO K 152 (RTSBK). The RTSBK positive samples were further tested by polyacrylamide gel electrophoresis and the silver staining technique to detect rotavirus dsRNA. Risk factors were identified by multivariable logistic regression analysis. The G and P genotypes of BRV were determined by RT-PCR and sequencing. A phylogenetic tree was constructed based on the neighbor-joining method using CLC sequence viewer 8.0. About 23% of the diarrheic calves were BRV positive. The odds of BRV infection were 3.8- (95% confidence interval [95% CI]: 1.0–14.7) and 3.9-times (95% CI:1.1–14.2) higher in Barisal and Madaripur districts, respectively, than Sirrajganj. The risk of BRV infection was 3.1-times (95% CI: 1.5–6.5) higher in calves aged ≤ 5 weeks than those aged >5 weeks. Moreover, the risk of BRV infection was 2.6-times (95% CI:1.1–5.8) higher in crossbred (Holstein Friesian, Shahiwal) than indigenous calves. G6P[11] was the predominant genotype (94.4%), followed by G10P[11] (5.6%). The BRV G6 strains were found to be closest (98.9–99.9%) to Indian strains, and BRV G10 strains showed 99.9% identities with Indian strain. The VP4 gene of all P[11] strains showed >90% identities to each other and also with Indian strains. The most frequently identified BRV genotype was G6P[11]. About 23% of calf diarrhea cases were associated with BRV. To control disease, high-risk areas and younger crossbred calves should be targeted for surveillance and management. The predominant genotype could be utilized as the future vaccine candidate or vaccines with the dominant genotype should be used to control BRV diarrhea in Bangladesh.

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“…A previous study by using rapid test on calve fecal samples showed in Babylon, Karbala ,Najaf, AL-Qadisyia revealed that infection rate were 60%, 20% , 40% and 33% respectively (10). Chromatographic Immunoassay and ELISA are the simple and good standard methods for detection of rotavirus.…”

Section: Resultsmentioning

confidence: 99%

Isolation and detection of rotavirus by Enzyme linked Immune assay in fecal specimens of buffalo calves

Khalaf

Aldoori²

2019

Iraqi J. Vet. Med.

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A total of 50 fecal samples were collected from buffalo calves ages between 3 days to 4 months, in middle area of Iraq (Baghdad, Salahaldden, Babylon, Diyala, and Wasit) between July 2016 to June 2017. All samples were examined by Immuno-chromatographic rapid tests for detection of buffalo rotavirus, twenty samples were positive. Enzyme-Linked Immunosorbent Assay. test was also used and revealed only 8 positive samples, the later samples were used for viral isolation on fetal bovine kidney cell culture for detection the cytopathogenic effects of the virus. The cytopathogenic effects of virus were induced rounded cells and gaps; as a result of rupture of these infected cells by rotavirus. Moreover Enzyme-Linked Immunosorbent Assay test used again for detection of rotavirus cytopathogenic effect in fetal bovine kidney cell culture for more confirmation. The TCID 50 was estimated for rotavirus on fetal bovine kidney cells 104.5 virus/0.05 ml

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Detection of bovine rotavirus in diarrheic calves by using rapid test in some Mid-Euphrates provinces

Cited by 4 publications

References 20 publications

Epidemiological and molecular study of Rotavirus infection among human and animal in Karbala and Basrah provinces

Epidemiological and molecular study of Rotavirus infection among human and animal in Karbala and Basrah provinces

Risk factors for bovine rotavirus infection and genotyping of bovine rotavirus in diarrheic calves in Bangladesh

Isolation and detection of rotavirus by Enzyme linked Immune assay in fecal specimens of buffalo calves

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