Detection of bat coronaviruses from Miniopterus fuliginosus in Japan

Abstract

Bats have great potential as reservoirs for emerging viruses such as severe acute respiratory syndrome-coronavirus. In this study, bat coronaviruses (BtCoVs) were detected by RT-PCR from intestinal and fecal specimens of Miniopterus fuliginosus breeding colonies in Wakayama Prefecture caves, where we previously identified bat betaherpesvirus 2. Two primer sets were used for the detection of BtCoV: one was for the RNA-dependent RNA polymerase (RdRp) region and the other was for the spike (S) protein region. Eleven and 73% of intestinal and fecal specimens, respectively, were positive for RdRp region, and 2 and 40% of those were positive for S protein region. Sequencing and phylogenetic analysis showed that the detected BtCoV belonged to the group 1 (alpha) coronaviruses. These data suggest that BtCoV is endemic in M. fuliginosus in Japan.

Hepatitis Virus in Long-Fingered Bats, Myanmar

“…These bats have a wide distribution ( 8 ), and increasing numbers of viruses, including coronaviruses and betaherpesviruses, are being isolated from them ( 9 , 10 ). Of the 6 bat species we sampled, only M. fuliginosus was positive for BtHV.…”

Middle East Respiratory Syndrome Coronavirus Infection Not Found in Camels in Japan

“…Saliva and nasal swabs were collected using UTM Virus Collection (360C, Copan Diagnosis, Brescia, Italy) depending on the breeding condition. Detection of MERS-CoV was performed by 2 MERS-CoV-specific assays-real-time RT-PCR (upE and ORF1 a probe sets) (10,11) and reverse transcription-loop-mediated isothermal amplification (RT-LAMP) (12) and a comprehensive assay using conventional RT-PCR using a primer set for pancoronaviruses (13). Viral RNAs were extracted using the QIAamp Viral RNA Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions.…”

“…The supernatants were used for RNA isolation. The protocols for RT-PCR were previously described (10)(11)(12)(13). Five microliters of RNA were used for each amplification reaction.…”

<b>Ocorrência e caracterização molecular de coronavírus e rotavírus do grupo A em quirópteros do Estado de São Paulo</b>

“…Sendo que a primeira já é conhecidamente importante na população de morcegos e está distribuída no mundo todo (TANG et al, 2006;;WOO et al, 2006;CARRINGTON et al, 2008;DOMINGUEZ et al, 2008;GLOZA-RAUSCH et al, 2008;TONG et al, 2009;AUGUST;MATHEWS;NUNN, 2012;CARRINGTON et al, 2008;SHIRATO et al, 2012b;ANTHONY et al, 2013;GÓES et al, 2013;LELLI et al, 2013), enquanto que a última ainda foi pouco explorada, apresentando duas descrições na China (HE et al, 2013;XIA et al, 2014), uma no (WOO et al, 2006;DOMINGUEZ et al, 2008;GLOZA-RAUSCH et al, 2008;WATANABE et al, 2010;AUGUST;MATHEWS;NUNN, 2012;SHIRATO et al, 2012b;LELLI et al, 2013). Não foi verificada associação da ocorrência de coronavírus e sexo do animal (p=0,869), como já descrito por anteriormente (GLOZA-RAUSCH et al, 2008;OSBORNE et al, 2011).…”

“…Ainda, o ectodomínio S1 é a porção que possui a maioria dos epítopos neutralizantes, induzindo a resposta imune hospedeira podendo ser utilizada como subunidade de vacina contra a infecção por coronavírus ( HE et al, 2005;DU et al, 2009) Os coronavírus usam uma grande variedade de receptores, incluindo os receptores da enzima conversora de angiotensina usada pelo SARS-CoV HOFMANN et al, 2005) e HCoV-NL63; da aminopeptidase N usada pelo TGEV (DELMAS et al, 1992) GLOZA-RAUSCH et al, 2008;MISRA et al, 2009;TONG et al, 2009; OSBORNE et al, 2011;SHIRATO et al, 2012b;CORMAN et al, 2013;LELLI et al, 2013). Dentro desses dois gêneros, novas espécies virais obtidas de morcegos estão sendo determinadas, demonstrando a importância desses animais como reservatórios de coronavírus (DE GROOT et al, 2012).…”