Detection of Antinuclear Antibody in Childhood Rheumatic Diseases by Immunofluorescence Assay and Enzyme Immuno Assay

Dipti, Tanjeem Rabika; Azam, Mohammad Shaiful; Sattar, Mohammad Humayun; Rahman, Shahana A

doi:10.3329/bjch.v35i2.10355

Cited by 3 publications

(5 citation statements)

References 10 publications

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“…Regarding autoantibody profiles, ANA was positive among all the patients (100%), and results of other studies such as those by Dipti et al and Weiss, who found 100% and 99% ANA positivity, respectively, 29 , 30 matched with our results, but ANA (IIF) was positive in 94% of SLE patients in the study by Gomes et al 19 Meanwhile, anti-dsDNA was detected in 83% of the samples in our study. Sahin et al found a similar result (84.8%), 24 but a lower percentage was reported by Balci et al (45.3%).…”

Section: Discussionsupporting

confidence: 90%

“…Variable results were obtained from different studies; Balci et al found that anti-CL IgG and IgM were positivity seen in 8 (15.1%) and 11 (20.8%) patients, respectively, 17 while Hiraki et al and Kim et al found that antiphospholipid antibodies were present in 32% and 42.8% of patients, respectively. 25,28 Regarding autoantibody profiles, ANA was positive among all the patients (100%), and results of other studies such as those by Dipti et al and Weiss, who found 100% and 99% ANA positivity, respectively, 29,30 matched with our results, but ANA (IIF) was positive in 94% of SLE patients in the study by Gomes et al 19 Meanwhile, anti-dsDNA was detected in 83% of the samples in our study. Sahin et al found a similar result (84.8%), 24 but a lower percentage was reported by Balci et al (45.3%).…”

Section: Discussionsupporting

confidence: 89%

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Autoantibody Profile of Egyptian Juvenile Systemic Lupus Erythematosus Patients and Its Association with Clinical Characteristics and Disease Activity

Teama¹,

El-Mohamdy²,

Mahmoud³

et al. 2021

OARRR

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This study was conducted to estimate the frequency of anti-nuclear antibodies (ANAs), anti-dsDNA, and anti-extractable nuclear antigen (ENA) antibodies in juvenile systemic lupus erythematosus (JSLE) patients and their association with different clinical manifestations and disease activity. Patients and Methods: A cross-sectional study that includes 100 JSLE patients from Ain Shams University Hospital was conducted. All subjects underwent history taking, clinical examination, assessment of disease activity based on the SLE disease activity index (SLEDAI), laboratory investigations, and tests for autoantibodies, namely ANA, anti-dsDNA, and anti-ENA antibodies, including anti-Ro (SSA), anti-La (SSB), anti-Smith (Sm), and anti-U1-ribonucleoprotein (U1-RNP). Results: The most common clinical features were polyarthralgia (71%), haematological manifestations (65%), malar rash (54%), and nephritis (51%), respectively. All patients had positive ANA (100%), while anti-dsDNA frequency was 83%. The most common anti-ENA antibodies were anti-RNP (41%), anti-Sm (31%), anti-SSA (27%), and anti-SSB (20%), respectively. Anti-RNP had a clinical association with oral ulcer, Raynaud' phenomena, haematological, neuropsychiatric and thromboembolic manifestations. Meanwhile, anti-Sm had a significant association with serositis, mucocutaneous, constitutional, and neuropsychiatric manifestations. Anti-SSA was associated with mucocutaneous, musculoskeletal, Raynaud' phenomena, renal, haematological and cardiac manifestations, while anti-SSB was significantly associated with malar rash, serositis, thromboembolic, musculoskeletal, and neuropsychiatric manifestations. Concerning SLEADI score, anti-dsDNA antibody was significantly associated with moderate disease activity score (p=0.032) while anti-SSA significantly associated with high disease activity (p=0.045). Both anti-SSB and anti-Sm were significantly associated with both moderate and high disease activities, meanwhile anti-U1-RNP was associated with moderate disease activity (p=0.014). Conclusion:Anti-dsDNA and anti-ENAs antibodies were frequently found in JSLE patients (83%, 63%), respectively. They were significantly associated with variable clinical manifestations and could be used as predictors for assessment of disease activity.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 89%

Autoantibody Profile of Egyptian Juvenile Systemic Lupus Erythematosus Patients and Its Association with Clinical Characteristics and Disease Activity

Teama¹,

El-Mohamdy²,

Mahmoud³

et al. 2021

OARRR

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“…In this study ANA (IIF) was present in 94% SLE patients where Dipti et al [19] and Weiss [20] found 100% and 99% ANA positivity by IIF method respectively in their study. Homogenous pattern of ANA was found as the predominant (54%), followed by fine speckled (18%) and coarse speckled (14%) pattern in the present study.…”

Section: Discussionmentioning

confidence: 53%

Autoantibody Profile and Its Association with Clinical Presentation of Paediatric SLE Patients

Gomes¹,

Islam²,

Rahman³

2019

AJP

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Systemic lupus erythematosus (SLE) is a chronic autoimmune disease characterized by the production of various auto antibodies including anti-nuclear antibody, double stranded DNA and antibodies to extractable nuclear antigens (ENA), which include four groups of RNA binding protein namely Sm, RNP, SSA/Ro and SSB/La. The objectives were to detect the frequency and pattern of ANA, frequency of anti-ENA antibodies and association between the clinical presentations with ANA and Anti-ENA antibodies in paediatric SLE patients. It was a cross sectional study and the data was collected from patients fulfilling the ACR 1997 revised criteria of SLE in the Department of Paediatrics, BSMMU, Dhaka, Bangladesh. Among 50 paediatric SLE patients, ANA was positive in 94% of the patients. Homogenous pattern of ANA was the highest (57%) followed by speckled (31%), cytoplasmic (6.6%) and nucleolar (3.3%) patterns. Anti-ds DNA positivity was 86% and anti-ENA positivity was 56% in this study. Among them, anti-RNP was found in 43.5% followed by anti-sm (24%), anti-SSA (24%) and anti-SSB (17%). Anti-RNP antibody was associated with renal and gastrointestinal and Anti-SSA was associated with only gastrointestinal manifestations. ANA, anti ds-DNA, anti ENA positivity were found significantly among paediatric SLE patients and clinical presentation of them had association with Anti-ENA antibodies.

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“…Autoimmune diseases are conditions in which the immune system damages specific organs or tissues and are characterized by the presence of antinuclear antibodies (ANA) in the blood of the patients. ANA are specific antibodies directed against a variety of nuclear antigens which have the capability of binding and destroying certain structures within the nucleus of the cells and are detected in the serum of the patients with rheumatic & non rheumatic diseases [1]. Although lower amounts of these antibodies are seen in normal individuals, an increase in titres are exclusively seen in patients with autoimmune diseases and serve as markers for diagnosis and prognosis [2].…”

Section: Introductionmentioning

confidence: 99%

“…Although lower amounts of these antibodies are seen in normal individuals, an increase in titres are exclusively seen in patients with autoimmune diseases and serve as markers for diagnosis and prognosis [2]. These antibodies are involved not only in disease pathogenesis but also constitute basis for diagnosis and treatment [1]. Their detection with high sensitivity and specificity is therefore of utmost importance.…”

Section: Introductionmentioning

confidence: 99%

Detection of antinuclear antibodies by indirect immunofluorescence method and its comparison with line immunoassay in a tertiary care hospital: A laboratory based observational study

B¹,

B²

2014

J Med Sci Res.

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Antinuclear antibodies (ANA) are the hallmark of autoantibody production in autoimmune diseases and its testing is widely used as screening test in autoimmune diseases. ANA are directed against components of the cell nuclei such as DNA, histones, nucleoli and ribonucleoprotein. ANA are detected by indirect immunofluorescence (IIF) assay, which is among the most commonly used routine method for ANA detection as screening test, due to its ability to detect multiple antigens simultaneously. In this study, serum samples, referred to our laboratory for ANA testing were subjected for testing by IIF method and line immunoassay (LIA) during a study period of 20 months and the two were correlated with one another to establish any link between the two. A total of 279 serum samples were processed for ANA testing during the study period from June 2012 to January 2014. Of these 279 samples, 199(71.3%) were ANA IIF positive and 80(28.7%) were ANA IIF negative. The spectrum of various positive ANA IIF patterns are nucleus homogenous 52(26.1%), nucleus granular 50(25.1%), mixed pattern 57(28.6%), mitosis positive 15(7.5%), nucleus nucleolar 13(6.5%), nucleus dotted 8(4%), nuclear membrane 1(0.5%), cytoplasm positive 3(1.5%). All the samples tested by ANA IIF were subjected to LIA. Of these 159(56.9%) were both ANA IIF and LIA positive. In addition, 40(14.3%) samples were detected as IIF positive but LIA negative, whereas the rest 14(5%) samples were IIF negative but LIA positive. In the present study, a definite correlation was found in 201(71%) samples between ANA patterns and the LIA. Thus ANA IIF method using biochips can be used as a cost effective screening method for ANA testing and restricting LIA, which are expensive. This could economize on the cost of laboratory investigations in a developing country like India.

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Detection of Antinuclear Antibody in Childhood Rheumatic Diseases by Immunofluorescence Assay and Enzyme Immuno Assay

Cited by 3 publications

References 10 publications

Autoantibody Profile of Egyptian Juvenile Systemic Lupus Erythematosus Patients and Its Association with Clinical Characteristics and Disease Activity

Autoantibody Profile of Egyptian Juvenile Systemic Lupus Erythematosus Patients and Its Association with Clinical Characteristics and Disease Activity

Autoantibody Profile and Its Association with Clinical Presentation of Paediatric SLE Patients

Detection of antinuclear antibodies by indirect immunofluorescence method and its comparison with line immunoassay in a tertiary care hospital: A laboratory based observational study

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