Detection of Animal Species in Some Meat and Meat Products by Comparatively Using DNA Microarray and Real Time PCR Methods

Özpınar, Haydar; Tezmen, Gündüz; Gökçe, İnci; Tekiner, İsmail Hakkı

doi:10.9775/kvfd.2012.7616

Cited by 16 publications

(14 citation statements)

References 41 publications

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“…However, previous market studies in Mexico, Turkey, and South Africa have reported mislabeling rates of approximately 20e70% for a variety of meat products, including sausage, ground meat, meat balls, deli meats, and dried meats (Ayaz, Ayaz, & Erol, 2006;Cawthorn, Steinman, & Hoffman, 2013;D'Amato, Alechine, Cloete, Davison, & Corach, 2013;Flores-Munguia, BermudezAlmada, & Vazquez-Moreno, 2000;Ozpinar, Tezmen, Gokce, & Tekiner, 2013). For example, a South African study testing processed meat products found that 68% of the samples contained species that were not declared on the package labels (Cawthorn et al, 2013).…”

Section: Introductionmentioning

confidence: 91%

Identification of species in ground meat products sold on the U.S. commercial market using DNA-based methods

2016

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a b s t r a c tThe objective of this study was to test a variety of ground meat products sold on the U.S. commercial market for the presence of potential mislabeling. Forty-eight ground meat samples were purchased from online and retail sources, including both supermarkets and specialty meat retailers. DNA was extracted from each sample in duplicate and tested using DNA barcoding of the cytochrome c oxidase I (COI) gene. The resulting sequences were identified at the species level using the Barcode of Life Database (BOLD). Any samples that failed DNA barcoding went through repeat extraction and sequencing, and due to the possibility of a species mixture, they were tested with real-time polymerase chain reaction (PCR) targeting beef, chicken, lamb, turkey, pork and horse. Of the 48 samples analyzed in this study, 38 were labeled correctly and 10 were found to be mislabeled. Nine of the mislabeled samples were found to contain additional meat species based on real-time PCR, and one sample was mislabeled in its entirety. Interestingly, meat samples ordered from online specialty meat distributors had a higher rate of being mislabeled (35%) compared to samples purchased from a local butcher (18%) and samples purchased at local supermarkets (5.8%). Horsemeat, which is illegal to sell on the U.S. commercial market, was detected in two of the samples acquired from online specialty meat distributors. Overall, the mislabeling detected in this study appears to be due to either intentional mixing of lower-cost meat species into higher cost products or unintentional mixing of meat species due to cross-contamination during processing.

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Section: Introductionmentioning

confidence: 91%

Identification of species in ground meat products sold on the U.S. commercial market using DNA-based methods

2016

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“…Other studies have reported mislabeling rates of 20e70% for various meat products, including ground meat, deli meats, pet foods, and dried meats (Ayaz, Ayaz, & Erol, 2006;Cawthorn, Steinman, & Hoffman, 2013;Flores-Munguia, Bermudez-Almada, & Vazquez-Moreno, 2000;Mousavi et al, 2015;Okuma & Hellberg, 2015;Ozpinar, Tezmen, Gokce, & Tekiner, 2013;Pascoal, Prado, Castro, Cepeda, & Barros-Vel azquez, 2004;Quinto, Tinoco, & Hellberg, 2016).…”

Section: Introductionmentioning

confidence: 96%

Identification of meat and poultry species in food products using DNA barcoding

Hellberg

Hernandez

2017

Food Control

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a b s t r a c t DNA barcoding is a promising method for the sequencing-based identification of meat and poultry species in food products. However, DNA degradation during processing may limit recovery of the fulllength DNA barcode from these foods. The objective of this study was to investigate the ability of DNA barcoding to identify species in meat and poultry products and to compare the results of full-length barcoding (658 bp) and mini-barcoding (127 bp). Sixty meat and poultry products were collected for this study, including deli meats, ground meats, dried meats, and canned meats. Each sample underwent full and mini-barcoding of the cytochrome c oxidase subunit I (COI) gene. The resulting sequences were queried against the Barcode of Life Database (BOLD) and GenBank for species identification. Overall, fullbarcoding showed a higher sequencing success rate (68.3%) as compared to mini-barcoding (38.3%). Mini-barcoding out-performed full barcoding for the identification of canned products (23.8% vs. 19.0% success), as well as for turkey and duck products; however, the primer set performed poorly when tested against chicken, beef, and bison/buffalo. Overall, full barcoding was found to be a robust method for the detection of species in meat and poultry products, with the exception of canned products. Minibarcoding shows high potential to be used for species identification in processed products; however, an improved primer set is needed for this application.

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“…Real time PCR has significant advantages over other methods [20,35] . On the other hand, success of a Real time PCR is dependent on a well-performed DNA extraction from test matrix [25,36] .…”

Section: Discussionmentioning

confidence: 99%

Türkiye Trakya Bölgesindeki Süt İşletmelerinden Toplanan Fekal ve Çiğ Süt Örneklerinde Mycobacterium avium subsp. paratuberculosis (MAP) İncelemesi

Özpınar¹,

Tekiner²,

Karaman³

et al. 2015

Kafkas Univ Vet Fak Derg

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The Thrace, which is Turkey's European part as well as the adjoining parts of Southern Bulgaria and north-east Greece has a strategic importance for being a vaccination buffer zone for Europe as declared by FAO in 1999. The objective of the study was to better understand the occurence of MAP in this animal disease free area of Turkey by applying F57 Real time PCR assay and IS900 nested-PCR. In this study, 270 feces samples from the dairy cattles over 2 years old in 30 randomly selected dairy farms, 45 raw milk samples from each of the bulk tanks belonging to these dairy farms and the villages located in Thrace were collected. Nine fecal samples were used to create the pooled fecal sample for a dairy farm before performing DNA extraction. All the samples were initially subjected to a F57-Real time PCR analysis, and subsequently an insertion sequence IS900 nested-PCR was performed to verify the results. However, the results revealed that MAP genom could not be detected in any pooled fecal and milk samples. In conclusion, the occurrence of MAP in this part of Turkey may likely be very lower than the capability of the detection limit of the used Real time PCR assay. Furthermore, the results once more confirmed the difficulty of MAP detection in asymptomatic animals and milk samples by performing PCR technique only. Keywords

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Detection of Animal Species in Some Meat and Meat Products by Comparatively Using DNA Microarray and Real Time PCR Methods

Cited by 16 publications

References 41 publications

Identification of species in ground meat products sold on the U.S. commercial market using DNA-based methods

Identification of species in ground meat products sold on the U.S. commercial market using DNA-based methods

Identification of meat and poultry species in food products using DNA barcoding

Türkiye Trakya Bölgesindeki Süt İşletmelerinden Toplanan Fekal ve Çiğ Süt Örneklerinde Mycobacterium avium subsp. paratuberculosis (MAP) İncelemesi

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