Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study

Calvet, Guilherme Amaral; Aguiar, Renato Santana; Melo, Adriana; Sampaio, Simone Alves; Filippis, Ivano de; Fabri, Allison; Araújo, Eliane Saraiva Machado de; Sequeira, Patrícia Carvalho de; Mendonça, Marcos César Lima de; Oliveira, Luciana de; Tschoeke, Diogo A.; Schrago, Carlos G.; Thompson, Fabiano L.; Brasil, Patrícia; Santos, Flávia Barreto dos; Nogueira, Rita Maria Ribeiro; Tanuri, Amílcar; Filippis, Ana María Bispo de

doi:10.1016/s1473-3099(16)00095-5

Cited by 1,028 publications

(880 citation statements)

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“…Although ZIKV infection has been confirmed in numerous cases of microcephaly [14,71,72], how many of these were actually caused by ZIKV infection has not been confirmed. Furthermore, the ZIKV genome has also been identified in pathological specimens of first trimester fetal miscarriages [14,71], although it has not been confirmed whether ZIKV was the cause of these fetal losses.…”

Section: Intrauterine (Congenital) Infectionmentioning

confidence: 99%

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Zika virus disease: a current review of the literature

et al. 2016

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Section: Intrauterine (Congenital) Infectionmentioning

confidence: 99%

“…During the period from March 2015 to February 2016, a greater than 20-fold increase in microcephaly cases was observed in Brazilian newborns compared to previous years [22]. A recent retrospective analysis from Brazil revealed 17 cases of central nervous system-related malformations among neonates [76].…”

Section: Intrauterine (Congenital) Infectionmentioning

confidence: 99%

Zika virus disease: a current review of the literature

et al. 2016

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“…Contamination to vertebrates occurs through the bite of infected mosquitos, which procreate and become infected by blood feeding in another viremic host. Nevertheless, new reports have led to the belief that ZIKV transmission may also occur through different mechanisms, including via placenta, sexually, or perinatally 5,13 . Moreover, descriptions of perinatal transmission have been found in individuals infected with CHIKV 2 .…”

Section: Uncontrolled Common Vector Transmission Worldwidementioning

confidence: 99%

“…Among them, 42 cases (57%) presented with Guillain-Barré syndrome. Additionally, there were reports of encephalitis, meningoencephalitis, facial paralysis, and acute myelitis associated with ZIKV infection 3,5,8 . In February 2016, the World Health Organization declared ZIKV infection a public health emergency, issuing a global alert about its relationship to cases of microcephaly, considering that the viral genome was found in blood samples and tissues of microcephalic neonates 27 .…”

Section: Warning Of Neurological Complications Of Dengue Zika and Cmentioning

confidence: 99%

“…In Brazil, the first report of autochthonous transmission of ZIKV was confirmed in May 2015, in the state of Bahia. It was then established that genomic characteristics identified in the country were similar to the virus isolated in French Polynesia, during the outbreak of 2013 5,7 . From 2013 to February 2016, cases of ZIKV infection have been registered in 28 countries in Latin America and the Caribbean 8 .…”

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confidence: 97%

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Dengue infection in the nervous system: lessons learned for Zika and Chikungunya

Puccioni-Sohler

Roveroni

Rosadas

et al. 2017

Arq. Neuro-Psiquiatr.

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Dengue, Zika and Chikungunya are emerging arboviruses and important causes of acute febrile disease in tropical areas. Although dengue does not represent a new condition, a geographic expansion over time has occurred with the appearance of severe neurological complications. Neglect has allowed the propagation of the vector (Aedes spp), which is also responsible for the transmission of other infections such as Zika and Chikungunya throughout the world. The increased number of infected individuals has contributed to the rise of neurological manifestations including encephalitis, myelitis, meningitis, Guillain-Barré syndrome and congenital malformations such as microcephaly. In this narrative review, we characterize the impact of the geographic expansion of the vector on the appearance of neurological complications, and highlight the lack of highly accurate laboratory tests for nervous system infections. This represents a challenge for public health in the world, considering the high number of travelers and people living in endemic areas.

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Cognitive Development of Infants Exposed to the Zika Virus in Puerto Rico

et al. 2019

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IMPORTANCE Existing research has established a causal link between Zika virus (ZIKV) infection and severe birth defects or consequent health impairments; however, more subtle cognitive impairments have not been explored. OBJECTIVE To determine whether infants of mothers with at least 1 positive ZIKV test show differences in cognitive scores at ages 3 to 6 months and ages 9 to 12 months. DESIGN, SETTING, AND PARTICIPANTS This cross-sectional study recruited infants enrolled in existing ZIKV study cohorts associated with the Maternal-Infant Studies Center and the Puerto Rico Clinical and Translational Research Consortium at the University of Puerto Rico and from the broader San Juan metropolitan area. The study took place at the Puerto Rico Clinical and Translational Research Consortium at the University of Puerto Rico. Participants were recruited through convenience sampling if their mothers underwent ZIKV testing prenatally and were at the target ages during the study period. Infants who were born preterm (<36 weeks' gestational age), with low birth weight (<2500 g), or with a known genetic disorder were excluded. Infants were tested from ages 3 to 6 months or ages 9 to 12 months from May 2018 to April 2019. Data analysis was performed from March to April 2019. EXPOSURES Zika virus status was measured prenatally and in the early postnatal period using realtime polymerase chain reaction or a ZIKV IgM antibody capture enzyme-linked immunosorbent assay. MAIN OUTCOMES AND MEASURES The infants' development was assessed using the Mullen Scales of Early Learning (translated to Spanish and adapted for Puerto Rico), and assessors were blinded to each infant's ZIKV status. RESULTS A total of 65 study participants were included. The mean (SD) age of the infants at the time of cognitive testing was 8.98 (3.19) months. Most of the infants were white (55 [84.6%]) and Puerto Rican (64 [98.5%]); 38 of the infants were male (58.5%). General cognitive and domainspecific scores did not differ significantly between prenatally ZIKV-positive and ZIKV-negative infants except for receptive language score (mean difference = 5.52; t = 2.10; P = .04). Exposure to ZIKV

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Detection and sequencing of Zika virus from amniotic fluid of fetuses with microcephaly in Brazil: a case study

Abstract: Consellho Nacional de Desenvolvimento e Pesquisa (CNPq), Fundação de Amparo a Pesquisa do Estado do Rio de Janeiro (FAPERJ).

Cited by 1,028 publications

References 29 publications

Zika virus disease: a current review of the literature

Zika virus disease: a current review of the literature

Dengue infection in the nervous system: lessons learned for Zika and Chikungunya

Cognitive Development of Infants Exposed to the Zika Virus in Puerto Rico

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