Detection and Quantification of the Capsular Polysaccharide of Burkholderia pseudomallei in Serum and Urine Samples from Melioidosis Patients

Abstract

Melioidosis is very challenging to diagnose. There is a clear need for a point-of-care assay for the detection of B. pseudomallei antigen directly from patient samples.

Electrochemical Immunoassay for Capturing Capsular Polysaccharide of Burkholderia pseudomallei: Early Onsite Detection of Melioidosis

“…33,34 One study found that the detection of CPS in patient samples was possible, however, the sensitivity in serum and urine was low at 6.5% and 40.5% respectively. 15 A second study confirmed the low sensitivity of detecting CPS in serum samples and discussed that in resource-limited situations, the detection of CPS via LFI in serum may be useful. 23 Another study investigated LFI-based detection of CPS with various monoclonal antibodies and found that mAb 4C4 had a selective and sensitive affinity for CPS.…”

“…A calibrated dose response was developed using CPS-spiked serum or urine samples, and recovery was estimated from a linear graph. Biosensor properties such as dynamic linear range, accuracy, sensitivity, specificity, cross-reactivity, and interference were in each buffer and the results were compared to a CPS antigen-capture ELISA that has been previously described . The Bland-Altman test was performed to compare the results obtained using the READ sensor with ELISA.…”

“…One study found that the detection of CPS in patient samples was possible, however, the sensitivity in serum and urine was low at 6.5% and 40.5% respectively . A second study confirmed the low sensitivity of detecting CPS in serum samples and discussed that in resource-limited situations, the detection of CPS via LFI in serum may be useful .…”

“…This study aims to continue to advance melioidosis diagnostic research by employing a novel biosensing technology. In recent decades, an exploration into the capsular polysaccharide (CPS) of B. pseudomallei has gained interest both as a target for potential diagnostics and human immunization. − CPS is a virulence factor that is displayed on the outer membrane of the bacterium, it has been shown to prevent phagocytosis and promote colonization . B. pseudomallei CPS structure is an unbranched homopolymer of 1,3-linked 2- O -acetyl-6-deoxy- b -D-manno-heptopyranose .…”

“…In vivo, the secreted form is quickly cleared through urinary excretion . Detection and quantification of these capsular polysaccharides have been identified as a potential diagnostic approach, ,− while their inhibition and isolation have the potential for immunization , and treatment, making them an attractive contender for biomarker detection and research …”