Detection and Quantification of &lt;em&gt;Plasmodium falciparum&lt;/em&gt; in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Martin, Miguela; Pérez-Guaita, David; Andrew, Dean; Richards, James L.; Wood, Bayden Robert; Heraud, Philip

doi:10.3791/56797-v

Cited by 2 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Martin et al (19) also demonstrated that FTIR spectra could be used to detect malaria in aqueous samples of red blood cells spiked with malaria infected. The method of sample preparation, spectral acquisition, and modeling was presented in a video format for the Journal of Visualization Experiments (19).…”

Section: Application Of Infrared Spectroscopy In Malaria Researchmentioning

confidence: 99%

Whole-Organism Analysis by Vibrational Spectroscopy

Christensen

Rüther

Kochan

et al. 2019

Annual Rev. Anal. Chem.

View full text Add to dashboard Cite

Vibrational spectroscopy has contributed to the understanding of biological materials for many years. As the technology has advanced, the technique has been brought to bear on the analysis of whole organisms. Here, we discuss advanced and recently developed infrared and Raman spectroscopic instrumentation to whole-organism analysis. We highlight many of the recent contributions made in this relatively new area of spectroscopy, particularly addressing organisms associated with disease with emphasis on diagnosis and treatment. The application of vibrational spectroscopic techniques to entire organisms is still in its infancy, but new developments in imaging and chemometric processing will likely expand in the field in the near future.

show abstract

Section: Application Of Infrared Spectroscopy In Malaria Researchmentioning

confidence: 99%

Whole-Organism Analysis by Vibrational Spectroscopy

Christensen

Rüther

Kochan

et al. 2019

Annual Rev. Anal. Chem.

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

“…Given the problems in current screening techniques, ATR FT-IR offers an innovative alternative due to its portability, ease of use and affordability, and its proven capacity to diagnose malaria infection. In order to determine the optimal spectral acquisition method and important parameters, both dry and aqueous data were collected as per the Roy et al 14 and the Martin et al 16 method.…”

Section: Introductionmentioning

confidence: 99%

“…In 2017, Roy et al developed a method to detect multiple analytes in addition to quantifying malaria parasitemia using a portable bench-top ATR FT-IR spectrometer, showing comparable sensitivity to the Khoshmanesh et al 13 method while simplifying the procedure for clinical use (Roy et al 14 ). In the same year, Martin et al 15,16 demonstrated that it was possible to quantify parasites in aqueous blood using ATR spectroscopy and that for lower parasitemia blood samples should be collected in lithium heparin anticoagulant tubes. In 2019, Heraud et al 17 applied the method in a larger-scale study, collecting samples from regional Thai clinics, n = 318, and demonstrated a high specificity and sensitivity using machine learning classification coupled with cloud-based data management.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Identification of Glucose-6 Phosphate Dehydrogenase Deficient Patients Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy Using Partial Least Squares Discriminant Analysis in Aqueous Blood Samples

et al. 2023

View full text Add to dashboard Cite

Glucose-6 phosphate dehydrogenase (G6PD) deficiency is an X-linked blood disease that affects 400 million people globally and is especially prevalent in malaria-endemic regions. A significant portion of carriers are asymptomatic and undiagnosed posing complications in the eradication of malaria as it restricts the types of drugs used for malaria treatment. A simple and accurate diagnosis of the deficiency is vital in the eradication of malaria. In this study, we investigate the potential of attenuated total reflection Fourier transform infrared spectroscopy (ATR FT-IR) as a diagnostic technique for G6PD deficiency. Venous blood samples were collected in lithium heparin anticoagulant tubes from G6PD partial and fully deficient volunteers, n = 17, and normal volunteers, n = 59, in Khon Kaen, Thailand. Spectra of aqueous and dry samples were acquired of whole blood, plasma, and red blood cells, and modeled using partial least squares discriminant analysis (PLS-DA). PLS-DA modeling resulted in a sensitivity of 0.800 and specificity of 0.800 correctly classifying fully deficient participants as well as a majority of partially deficient females who are often misdiagnosed as normal by current screening methods. The viability of utilizing aqueous samples has always been hindered by the variability of hydration in the sample, but by employing multicurve curve resolution-alternating least squares to subtract water from each sample we are able to produce high-quality spectra with minimized water contributions. The approach shows proof of principle that ATR FT-IR combined with multivariate data analysis could become a frontline screening tool for G6PD deficiency by improving tailored drug treatments and ultimately saving lives.

show abstract

Detection and Quantification of <em>Plasmodium falciparum</em> in Aqueous Red Blood Cells by Attenuated Total Reflection Infrared Spectroscopy and Multivariate Data Analysis

Cited by 2 publications

References 0 publications

Whole-Organism Analysis by Vibrational Spectroscopy

Whole-Organism Analysis by Vibrational Spectroscopy

Identification of Glucose-6 Phosphate Dehydrogenase Deficient Patients Using Attenuated Total Reflection Fourier Transform Infrared Spectroscopy Using Partial Least Squares Discriminant Analysis in Aqueous Blood Samples

Contact Info

Product

Resources

About