Biochemical Pharmacology volume 77, issue 10, P1612-1620 2009 DOI: 10.1016/j.bcp.2009.02.017 View full text
Laura Formentini, Flavio Moroni, Alberto Chiarugi

Abstract: The emerging key role of NAD-consuming enzymes in cell biology has renewed the interest in NAD resynthesis through the rescue pathways. The first step of the nicotinamide-dependent NAD-rescue pathway is operated by nicotinamide phosphoribosyl transferase (NaPRT) forming nicotinamide mononucleotide (NMN). Because of the difficulties in measuring NMN, numerous open questions exist about the pathophysiological relevance of NaPRT and NMN itself. Here, we describe a new method of fluorimetric NMN detection upon der…

expand abstract