2016
DOI: 10.1016/j.bjid.2016.07.001
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Detection and molecular characterization of β-lactamase genes in clinical isolates of Gram-negative bacteria in Southern Ecuador

Abstract: This work performed a phenotypic and genotypic characterization of 79 clinical isolates of Enterobacteriaceae and Pseudomonadaceae collected in hospitals of Southern Ecuadorin 2013. Our results showed a high incidence of β-lactamases and ESBLs with bla and bla as the prevalent genes, respectively. By direct sequencing of PCR amplicons, the different β-lactamases and variants of the genes were also distinguished. Our results revealed a predominance of TEM-1 β-lactamase and the presence of different CTX-M varian… Show more

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Cited by 22 publications
(24 citation statements)
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“…and Enterobacter spp. isolated in food, clinical, and environmental samples around the world as previously described (Smet et al, 2010;Delgado et al, 2016).…”
Section: Introductionmentioning
confidence: 99%
“…and Enterobacter spp. isolated in food, clinical, and environmental samples around the world as previously described (Smet et al, 2010;Delgado et al, 2016).…”
Section: Introductionmentioning
confidence: 99%
“…This new glimmer of hope was however ephemeral as resistant pathogens were soon detected afterwards. This emergence of resistant microorganisms to antibiotics has now been identified as a major global menace [1, 2, 3, 4, 5, 6, 7]. The major cause of this phenomenon is the spread of the plasmid–encoded Extended-Spectrum Beta-Lactamase (ESBL) genes, conferring resistance to third generation cephalosporins [8].…”
Section: Introductionmentioning
confidence: 99%
“…CTX-M enzymes, which contain over 80 CTX-M enzymes, have just about 40% identity with SHV and TEM. In South America, these enzymes are prevalently detected in E. coli and other species of Enterobacteriaceae (Delgado et al 2016). Delgado et al (2016) detected variants of CTX-M, with CTX-M-15 being the highest as well as 2 rare variants of CTX-M (CTX-M-15) which has also been reported in K. pneumoniae ATCC BAA-2146 in Asia (South Korea) (Cho et al 2015).…”
Section: Ctx-m Beta-lactamases (Class A)mentioning
confidence: 89%
“…In South America, these enzymes are prevalently detected in E. coli and other species of Enterobacteriaceae (Delgado et al 2016). Delgado et al (2016) detected variants of CTX-M, with CTX-M-15 being the highest as well as 2 rare variants of CTX-M (CTX-M-15) which has also been reported in K. pneumoniae ATCC BAA-2146 in Asia (South Korea) (Cho et al 2015). Environmental detection of these enzymes and its determinants, as well as other antibiotic resistance determinants previously believed to be in the exclusive preserve of the clinical isolates, calls for caution in handling environmental samples like the clinical samples (Adegoke and Okoh 2015).…”
Section: Ctx-m Beta-lactamases (Class A)mentioning
confidence: 99%