Detection and identification of mycobacteria by amplification of mycobacterial DNA

Hance, Allan J.; Grandchamp, Bernard; Lévy-Frébault, Véronique; Lecossier, Denise; Rauzier, Jean; Bocart, Denis; Gicquel, Brigitte

doi:10.1111/j.1365-2958.1989.tb00233.x

Cited by 282 publications

(147 citation statements)

References 24 publications

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“…The 383 bp sequence of the 65 KDa antigen of Mycobacterium tuberculosis was amplified 2,5 . After agarose gel electrophoresis, amplification products were transferred to nylon membrane by Southern blotting technique and hybridized with a specific M. tuberculosis probe 1 3 .…”

Section: Methodsmentioning

confidence: 99%

Polymerase chain reaction in the diagnosis of tuberculous meningitis: preliminary report

Machado

Livramento

Bydlowski

et al. 1994

Arq. Neuro-Psiquiatr.

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In this preliminary report the results of PCR for detection of DNA sequences (65 KDa antigen) of Mycobacterium tuberculosis in CSF samples from 20 patients are registered. In 10 patients there were clinical and laboratory findings suggesting the diagnosis of tuberculous meningitis (test group). In the other 10 patients, clinical and laboratory findings suggested meningitis or meningo-encephalitis from other etiologies (control group). In 7 patients from the test group antigenic DNA sequences of Mycobacterium tuberculosis were found in CSF by PCR; positive results were not registered in the control group.

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Section: Methodsmentioning

confidence: 99%

Polymerase chain reaction in the diagnosis of tuberculous meningitis: preliminary report

Machado

Livramento

Bydlowski

et al. 1994

Arq. Neuro-Psiquiatr.

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“…All positive colonies were subcultured and identified (according to colony morphology, growth temperature and pigment production). For further identification we performed biochemical tests for nitrate reduction, catalase, Tween 80 hydrolysis, amidase, aryl sulphatase, pyrazinamidase and urease (Kent and Kubica, 1985), and polymerase chain reaction (PCR) (Hance et al, 1989;Kunze et al, 1992). Isolates identified as members of the M. avium complex were also serotyped.…”

Section: Identification Of the Isolatesmentioning

confidence: 99%

Mycobacterial infection of pigs in Croatia

Cvetnić

Špičić

Benić

et al. 2007

Acta Veterinaria Hungarica

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During a five-year period (2000 to 2004) 74,342 pigs were tested by the intradermal tuberculin test in Croatia. Of them, 248 (0.33%) pigs were positive and 91 (0.12%) were found to be suspicious in 7 out of the 13 farms included in the study. Gross pathological changes characteristic of tuberculosis were observed in tuberculin-positive and/or suspicious swine. Mycobacterium was isolated from the lymph nodes of 183 out of 234 swine (78.2%). For better epidemiological understanding, isolates were typed by conventional methods, PCR and hybridisation. The results show that most of the isolates belonged to the Mycobacterium avium complex (175 isolates, 95.7%). Other isolates belonged to M. fortuitum (6 isolates, 3.3%), M. chelonae (1 isolate, 0.5%), and M. peregrinum (1 isolate, 0.5%). Isolated strains of the M. avium complex were identified as M. a. avium (37 isolates, 21.1%) and M. a. hominissuis (138 isolates, 78.9%).

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“…Rapid and sensitive tools for the diagnosis of tuberculosis have been developed recently. In this context, the amplification of specific nucleic acids by polymerase chain reaction (PCR) provides a means for both increasing the sensitivity of detection and reducing the time required to diagnose pulmonary tuberculosis [5,61. Several studies have reported the success of PCR for the detection of M. tuberculosis DNA in clinical specimens such as sputum [7-lo].…”

Section: Introductionmentioning

confidence: 99%

Detection of Mycobacterium Tuberculosis DNA in Blood of Patients with Acute Pulmonary Tuberculosis by Polymerase Chain Reaction and Non-Isotopic Hybridisation Assay

Prete

D'Alagni²,

Sabato³

et al. 1997

Journal of Medical Microbiology

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The detection of Mycobacterium tuberculosis DNA in peripheral blood mononuclear cells (PBMC) by PCR and non-isotopic hybridisation assay was evaluated for the laboratory diagnosis of pulmonary M. tuberculosis infection. The PCR technique was based on the presence of IS6120, a DNA sequence specific for M. tuberculosis, and performed on PBMC from 30 patients belonging to the fifth group of the American Thoracic Society (ATS) classification of tuberculosis. The identification of amplification products was confirmed after electrophoresis by hybridisation with a non-isotopic probe in a DNA enzyme immunoassay (DEIA). Of the 30 blood samples studied by the PCR-DEIA technique, 26 gave positive results and four gave negative results. Blood samples from 30 subjects in a control group were negative by this technique. The data suggest that PCR-DEIA of blood may provide a sensitive, specific and useful means of diagnosing mycobacterial infection.

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Detection and identification of mycobacteria by amplification of mycobacterial DNA

Cited by 282 publications

References 24 publications

Polymerase chain reaction in the diagnosis of tuberculous meningitis: preliminary report

Polymerase chain reaction in the diagnosis of tuberculous meningitis: preliminary report

Mycobacterial infection of pigs in Croatia

Detection of Mycobacterium Tuberculosis DNA in Blood of Patients with Acute Pulmonary Tuberculosis by Polymerase Chain Reaction and Non-Isotopic Hybridisation Assay

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