Detection and identification of a 16SrIII‐J subgroup phytoplasma associated with faba bean in Peru

Suarez, Gilberto Torres; Gamarra, Delia Gamarra; Villar, Charo Milagros; Munive, Sally L. Llacza; Satta, Eleonora; Carrasco-Lozano, Emerson Clovis; Bertaccini, Assunta

doi:10.1111/jph.12977

Cited by 6 publications

(3 citation statements)

References 32 publications

(34 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The presence of 16SrII-D subgroup phytoplasmas in faba bean has been previously reported in Sudan (Alfaro-Fernandez et al, 2012), Saudi Arabia (AL-Saleh and Amer, 2014; Omar, 2017), and Iran , but phytoplasma infecting faba bean was found belong to the 16SrIII group in Spain (Castro and Romero, 2004) and Peru (Torres Suarez et al, 2021).…”

Section: Table (1): the Incidence Of Phytoplasma On Different Crops D...mentioning

confidence: 64%

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Ahmed,

Kheder,

Hamouda

et al. 2024

Egyptian Journal of Phytopathology

View full text Add to dashboard Cite

Phytoplasma-related symptoms such as big bud, witches' broom, phyllody, virescence, flower proliferation, little leaves, yellowing, and stem fasciation were observed in 9 plant species during a field survey in Fayoum Governorate, Egypt, in 2021 and 2022. Disease incidence on various crops ranged from 1 to 21.3% in 2021, with the percentage rising in 2022. In 2022, the highest incidence percentage (23 % (was recorded on sesame while on cowpea the lowest percentage of incidence was recorded being (1.2%). A nested PCR assay employing the universal primer pair P1/P7 followed by R16F2n/R16R2 was used to detect the presence of phytoplasmas by amplifying a segment of the phytoplasma 16S rRNA gene. The resulting amplicons were purified and sequenced. The sequences were deposited in GenBank. Sequence comparison, phylogenetic study, and virtual RFLP analysis of 16S rRNA indicated the presence of diverse phytoplasma ribosomal groups and subgroups infecting different crops ( 16SrVI-A, 16SrIII-A, 16SrII-D, 16SrII-A, 16SrI-A, and 16SrI-B). The 16SrVI-A phytoplasmas were detected in symptomatic tomato plants, the 16SrII-D phytoplasmas subgroup was identified in sesame, faba bean, and cowpea exhibiting phyllody symptoms, the 16SrII-A subgroup was detected in cauliflower. The 16SrIII-A and 16SrI-A phytoplasmas subgroups were identified in alfalfa witches' broom and chamomile, respectively for the first time in Egypt. Moreover, subgroup 16SrI-B was detected for the first time in Egypt in chrysanthemum and cabbage. This study showed the genetic diversity of phytoplasma strains infecting a variety of crops in Fayoum Governorate.

show abstract

Section: Table (1): the Incidence Of Phytoplasma On Different Crops D...mentioning

confidence: 64%

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Ahmed,

Kheder,

Hamouda

et al. 2024

Egyptian Journal of Phytopathology

View full text Add to dashboard Cite

show abstract

“…The 16SrIII-J phytoplasma strain is widespread in other countries of South America such as Brazil (Montano et al, 2000), Argentina (Galdeano et al, 2013) and Chile (Quiroga et al, 2020) where it was detected in several herbaceous and woody horticultural species. Recently it was also identified in faba bean in Peru (Torres-Suarez et al, 2021) and insect vectors of this phytoplasma strain were identified as Paratanus exitiosus (Beamer) and Bergallia valdiviana Berg 1881 in Chile (Quiroga et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Diverse phytoplasmas associated with maize bushy stunt disease in Peru

et al. 2022

Self Cite

View full text Add to dashboard Cite

Corn plants showing symptoms of midribs chlorosis, and leaf reddening, short internodes, ear proliferation, and plant growth reduction were collected in Peru from fields in nine localities in the provinces of Huancayo, Chupaca, and Jauja in the Junín region, and tested to verify phytoplasma presence and identity. Primers amplifying the phytoplasma ribosomal 16S and ribosomal protein genes were used. The phytoplasma presence was detected in symptomatic samples from all the surveyed areas. The sequencing of the obtained amplicons indicated the presence of ‘Candidatus Phytoplasma asteris’ and ‘Ca. P. pruni’-related strains. A BLASTn search of sequenced genes showed that the two ‘Candidatus Phytoplasma’ strains identified in corn shares 100% and 99.82% identity with the ‘Ca. P. asteris’ strains from maize and 99.92% and 99.55% with ‘Ca. P. pruni’-related strains, respectively. The RFLP analyses allowed to enclose these phytoplasma strains in the 16SrI-B and 16SrIII-J subgroups; however, the two phytoplasmas were, in some cases, present in mixed infection. The 16SrIII-J phytoplasma is for the first time reported associated with the maize bushy stunt disease and this represent a relevant information for the disease epidemiology towards its appropriate management in the affected area.

show abstract

“…Phytoplasma has been shown to be associated with the faba bean. The most common symptoms of phytoplasma infections of the faba bean are phyllody, shortening of internodes, flower virescence, witches’ broom, small leaves, and yellowing symptoms [ 6 , 7 , 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Nanoinhibitory Impacts of Salicylic Acid, Glycyrrhizic Acid Ammonium Salt, and Boric Acid Nanoparticles against Phytoplasma Associated with Faba Bean

et al. 2022

View full text Add to dashboard Cite

Phytoplasmas are economically important plant pathogenic bacterial diseases, causing severe yield losses worldwide. In this study, we tested nanoformulations such as glycyrrhizic acid ammonium salt (GAS), salicylic acid (SA), and boric acid (BA) as novel antimicrobial agents inducing the resistance against the phytoplasma disease in faba bean. The nanoparticles (NP) were foliar-applied to naturally phytoplasma-infected faba bean with three concentrations from each of SA, GAS, and BA, under field conditions. Nested PCR (using universal primer pairs P1/P7 and R16F2n/R16R2) were reacted positively with all symptomatic samples and gave a product size of approximately 1200 bp, while the healthy plant gave no results. Transmission electron microscopy examinations of phytoplasma-infected faba bean plants treated with different nanoparticles revealed that severe damage occurred in phytoplasma particle’s structure, degradation, malformation, lysis in the cell membrane, and the cytoplasmic leakage followed by complete lysis of phytoplasma cells. Exogenous application of GAS-NP (1.68 µM), SA-NP (0.28 µM), and BA-NP (0.124 µM) suppressed the infection percentage of phytoplasma by 75%, 50%, and 20%, and the disease severity by 84%, 64%, and 54%, respectively. Foliar application of nanoparticles improved Fv/Fm (maximum quantum efficiency of PSII Photochemistry), PI (the performance index), SPAD chlorophyll (the relative chlorophyll content), shoots height, and leaves number, thus inducing recovery of the plant biomass and green pods yield. The most effective treatment was GAS-NP at 1.68 µM that mediated substantial increases in the shoots’ fresh weight, shoots’ dry weight, number of pods per plant, and green pods yield by 230%, 244%, 202% and 178%, respectively, compared to those of infected plants not sprayed with nanoparticles. This study demonstrated the utility of using nanoparticles, particularly GAS-NP at 1.68 µM to suppress the phytoplasma infection.

show abstract

Detection and identification of a 16SrIII‐J subgroup phytoplasma associated with faba bean in Peru

Cited by 6 publications

References 32 publications

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Prevalence and genetic diversity of phytoplasmas infecting different crops in Egypt

Diverse phytoplasmas associated with maize bushy stunt disease in Peru

Nanoinhibitory Impacts of Salicylic Acid, Glycyrrhizic Acid Ammonium Salt, and Boric Acid Nanoparticles against Phytoplasma Associated with Faba Bean

Contact Info

Product

Resources

About