2010
DOI: 10.1167/iovs.10-5712
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Abstract: With the use of these methods, bacterial detection was improved from 47.6% to 95.3%, demonstrating them to be sensitive, rapid tests for diagnosis of bacterial endophthalmitis.

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Cited by 67 publications
(49 citation statements)
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“…Our culture and gram stain negative endophthalmitis may have been solved with a more sensitive diagnostic technique such as polymerase chain reaction known as PCR. PCR has been found to significantly aid in bacterial and fungal detection [25,26]. In one real-time PCR study, detection of bacteria improved from 47.6% to 95.3%.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Our culture and gram stain negative endophthalmitis may have been solved with a more sensitive diagnostic technique such as polymerase chain reaction known as PCR. PCR has been found to significantly aid in bacterial and fungal detection [25,26]. In one real-time PCR study, detection of bacteria improved from 47.6% to 95.3%.…”
Section: Discussionmentioning
confidence: 99%
“…PCR has a greater level of false positive results. Also, specimens need to be properly stored in order for PCR to be effective [26].…”
Section: Discussionmentioning
confidence: 99%
“…Conventional microbiological culture of vitreous and aqueous humors followed by microscopic examinations are considered routine techniques for diagnosis of endophthalmitis. However, it has been reported that bacterial detection sensitivity via the aqueous and vitreous humor microbiological culture is low (25% to 56% of cases) [8][9][10]. Recently, newer molecular biology methods were developed with the purpose of ameliorating the range of microbiological diagnosis.…”
Section: Introductionmentioning
confidence: 99%
“…qPCR was performed on an ABI Prism 7500 DNA sequence detection system (Applied Biosystems, Waltham, MA, USA) and targeted the T. gondii 529-bp repetitive genomic sequence (rep529) (14,15) and the B1 gene (16) . β-globin qPCR was performed in parallel for each sample as described previously (17) in order to confirm the integrity of the DNA and to verify the PCR inhibitors.…”
Section: Real-time Qpcrmentioning
confidence: 99%