Detection and characterization of hepatitis A virus circulating in Egypt

Hamza, Hazem; Abd‐Elshafy, Dina Nadeem; Fayed, Sayed A.; Bahgat, Mahmoud Mohamed; El-Esnawy, Nagwa; Abdel-Mobdy, Emam

doi:10.1007/s00705-017-3294-4

Cited by 21 publications

(21 citation statements)

References 44 publications

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“…Previous studies were confirmed our data that genotype IB is the predominant genotype circulating in Egypt and North Africa (Robertson et al, 1992;Costa-Mattioli, 2007 andHamza et al, 2017). Furthermore, Hamza et al (2017) demonstrated the VP1/2A nucleotide sequences of all Egyptian isolates were 99.7% identical to each other and 96.4 to 97.6% identical to strains of the IB genotype.…”

Section: Discussionsupporting

confidence: 90%

“…Previous studies were conducted to evaluate HAV circulation in the Egyptian environment (Kamel et al, 2011 andHamza et al, 2017). Molecular characterization of HAV is important for understanding its circulation pattern and evolutionary relationships.…”

Section: Discussionmentioning

confidence: 99%

“…Subsequently, phylogenetic tree analysis using MEGA6 software (Tamura et al, 2013) revealed the high degree of nucleotide sequence similarity with HAVsubgenotypes IB (HAV/Egy/ST-NO-1/2015) KX228687, wild-type (HM-175) M14707and(IT-COL-00) AJ505564 with 99.10, 96.43 and 95.54%, respectively (Cohen et al, 1987;Chironna et al, 2004 andHamza et al, 2017 ). Previous studies were confirmed our data that genotype IB is the predominant genotype circulating in Egypt and North Africa (Robertson et al, 1992;Costa-Mattioli, 2007 andHamza et al, 2017). Furthermore, Hamza et al (2017) demonstrated the VP1/2A nucleotide sequences of all Egyptian isolates were 99.7% identical to each other and 96.4 to 97.6% identical to strains of the IB genotype.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown the predominance of genotype IB in Egypt (Pinto´ et al, 2007;Kamel et al, 2011 andHamza et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of Foodborne Hepatitis A virus Outbreaks in the Fresh Foods

et al. 2017

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HEPATITIS A virus (HAV) is one of the most widespread foodborne pathogens and the cause of viral hepatitis. Fresh foods can be considered as a vector of transmission for HAV when contaminated by spoiled irrigation water or when prepared by infected food handlers. To improve microbiological detection and to increase insights into the contribution of fruit and vegetables to foodborne viral transmission, sensitive and standardized methods are needed. Two outlet drainage water samples from El-Mariotia and El-Gable Elasfar canals (Nile River) were collected in each month starting from March 2015 over a period of 12 months. As well as vegetables and fruit samples (lettuce, green onion and strawberry) were irrigated and washed with drainage water were also collected. Samples were extracted and concentrated for viral analysis. Strawberry and lettuce foods collected from El-Mariotia and lettuce from El-Gable Elasfar gave positive serological results by enzyme-linked immunosorbent assay (ELISA) test. Reverse transcription polymerase chain reaction (RT-PCR) was successfully used to detect the virus in strawberry and lettuce samples using HAV-specific primers, designed to amplify a 500 bp fragment covering VP1/2A gene in HAV. The VP1/2A gene was sequenced and the nucleotide sequence similarity was in the range of 95-99.1% between HAV-Eg isolate and 30 HAV sequences retrieved from GenBank. Phylogenetic analysis revealed that HAV-Eg isolate was grouped into a clade comprising Egyptian HAV isolates sub-genotype IB. Phylogenetic tree of nucleotides sequence showed that HAV sub-genotype IB is the circulating strain.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown the predominance of genotype IB in Egypt (Pinto´ et al, 2007;Kamel et al, 2011 andHamza et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

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Monitoring of Foodborne Hepatitis A virus Outbreaks in the Fresh Foods

et al. 2017

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“…Formerly known as infectious hepatitis, hepatitis A is caused by the hepatitis A virus [1][2][3]. Hepatitis A virus (HAV) belongs to the genus Hepatovirus of the family of Picornaviridae [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Sero-prevalence and Risk Factors for Hepatitis A Virus Infection among Pregnant Women at the Samandin Medical Center, Ouagadougou, Burkina Faso

Hortense

Bienvenue

Abdoulaye

et al. 2019

JABB

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Aims: This study determined prevalence and possible risk factors associated with HAV infection and compared Immunochromatography (IgG) rapid point of care test with standard ELISA test for diagnosis. Study Design: Cross sectional epidemiological survey. Place and duration of Study: The study was carried out at the Samandin Medical Center (Ouagadougou, Burkina Faso), from July 2017 to December 2017. Methodology: A total of 180 pregnant women were recruited at the Samandin Medical Center. Anti-HAV IgM and IgG detection tests were performed using AccuDiag™ HAV ELISA kit (Calabasas, CA, USA) and SD BIOLINE HAV IgG/IgM Rapid Diagnostic Test (Gyeonggi-do, Korea). Results: IgG anti-HAV antibodies (AccuDiag Elisa) were found with a prevalence of 88.9% (160/180) and Anti-HAV IgM were 1.1% (2/180). Of the 160 AccuDiag HAV IgG positive samples, 44.4% (80/180) tested positive using SD BIOLINE Diagnostics HAV IgG. Regarding risk factors, 86.9% (86/99) of the IgG positive women were illiterate; 90.7% (39/43) lived mostly in peripheral areas; 89.2% (74/83) were household workers and 88.6% (93/105) used fountain water. Conclusion: Our results demonstrated poor agreement between the assays tested, which are consistent with previous reports demonstrating significant variability between HAV ELISA and RDT HAV. Moreover, the detection of HAV specific IgM antibodies in two asymptomatic pregnant women signaled the current circulation of HAV in this area.

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