2019
DOI: 10.1038/s41598-019-41045-z
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Abstract: Gastroenteritis in young animals is a clinical presentation with many infectious and non- infectious aetiologies. We used next generation sequencing (NGS) to investigate the possible infectious causes of gastroenteritis in puppies from a dog kennel in Victoria, Australia. The near complete genome of a canine astrovirus was obtained from pooled faecal samples, and was found to be 94.7% identical with a canine astrovirus detected in the United Kingdom in 2012. The phylogenetic analysis of the capsid gene found s… Show more

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Cited by 13 publications
(23 citation statements)
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References 47 publications
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“…All samples, both pooled and individual, were processed, and virus particles were enriched using a protocol described previously [36,37]. Briefly, the samples were homogenised at 25 Hz for 2 min, followed by centrifugation at 17,000× g for 3 min, and the supernatant was filtered using a 0.8 µm polyethersulfone (PES) spin-filter.…”
Section: Virus Enrichment Nucleic Acid Extraction Cdna Synthesis Amentioning
confidence: 99%
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“…All samples, both pooled and individual, were processed, and virus particles were enriched using a protocol described previously [36,37]. Briefly, the samples were homogenised at 25 Hz for 2 min, followed by centrifugation at 17,000× g for 3 min, and the supernatant was filtered using a 0.8 µm polyethersulfone (PES) spin-filter.…”
Section: Virus Enrichment Nucleic Acid Extraction Cdna Synthesis Amentioning
confidence: 99%
“…The samples were then divided into two, one aliquot ultracentrifuged at 178,000× g for 1 h and the other not ultracentrifuged. Both of the aliquots were Viruses 2020, 12, 88 3 of 13 then treated with benzonase and micrococcal nuclease for 2 h [36,37]. The ultracentrifuged samples were then treated with 200 µM PMAxx for 30 min in a PMA-Lit LED Photolysis Device, as indicated by the manufacturer (Biotium, Fremont, CA, USA).…”
Section: Virus Enrichment Nucleic Acid Extraction Cdna Synthesis Amentioning
confidence: 99%
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“…A pooled dog fecal sample (DFS) originating from two pups (7 weeks of age) was collected from the ground immediately after being deposited, with the consent of the owner 31 . These puppies were healthy at the time of collection, but they had been sick just 1-2 weeks before and an astrovirus was detected in the 7-week sample 31 . However, they were originally thought to have a Campylobacter infection and were likely treated with antibiotics at 5 weeks of age.…”
Section: Methodsmentioning
confidence: 99%