Detecting platform for phenolic compounds–characteristic of enzymatic electrode

Cabaj, Joanna; Chyla, A.; Jędrychowska, Agnieszka; Olech, Kamila; Sołoducho, Jadwiga

doi:10.1016/j.optmat.2012.02.042

Cited by 7 publications

(5 citation statements)

References 19 publications

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“…Because of the fact that the selenophene‐based polymers are less conductive in comparison to thiophene/ethylenedioxythiophene‐based macrostructures, the capacitive current is observed in diagram on Figure 8. This phenomenon was not observed in case of tyrosinase immobilized in conducting film built of co‐polymer based on bis(ethylenedioxythiohene)diphenylamine 36 reported by us earlier. Despite the fact, selenophene‐based polymers are known because of their high charge mobility and good solubility in organic solvents as well as good film‐forming properties 8 what is a significant benefit in biosensor modification.…”

Section: Resultsmentioning

confidence: 59%

“…Despite the fact, selenophene‐based polymers are known because of their high charge mobility and good solubility in organic solvents as well as good film‐forming properties 8 what is a significant benefit in biosensor modification. Moreover, the stability of the presented biosensor is more effective than reported in 36 (95 % retained after 3 month) whereas the value of the limit of detection was comparable.…”

Section: Resultsmentioning

confidence: 61%

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Tyrosinase Biosensor for Antioxidants Based on Semiconducting Polymer Support

et al. 2016

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This study reports sensitive phenolic compounds detection using biosensing electrode constructed by immobilization of tyrosinase in an electrochemically synthesized copolymer based on N‐nonylcarbazole derivatives on a platinum (Pt) electrode. Tyrosinase has been successfully immobilized (electrolytic deposition) on the surface of thin film built of poly[2,7‐bis(selenophene)‐N‐nonylcarbazole] and poly[3,6‐bis(selenophene)‐N‐nonylcarbazole]. A well‐defined reduction current according to the phenolic compounds was observed in cyclic voltammetry, which assigned to the reduction of biocatalytically produced o‐quinones on the electrode surface. The immersion of the tyrosinase‐equipped electrode in solution with substrate generated large catalytic currents easily recorded by cyclic voltammetry. The response of the biosensing arrangement was estimated in the presence of catechol and L‐3,4‐dihydroxyphenylalanine (L‐DOPA). The system exhibits an explicit catalytic activity and the substrates can be amperometrically determined at +0.07 V vs. Ag/AgCl. The activation energy (Ea) of immobilized tyrosinase catalytic reaction was estimated as 24.65 kJ/mol in PBS buffer. The analytical properties of the developed biosensor, such as linear concentration range, sensitivity, detection limit and reproducibility, repeatibility were also evaluated. Considering the fact, that the immobilization policy proved high efficiency, the results suggest that the method for phenoloxidase immobilization has a big capacity of providing high throughput engineering of bioelectronic devices.

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Section: Resultsmentioning

confidence: 59%

Section: Resultsmentioning

confidence: 61%

Tyrosinase Biosensor for Antioxidants Based on Semiconducting Polymer Support

et al. 2016

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“…This type of nanostructures contains graphene [10,11], nanoparticles (i.e. silver) [12], nanotubes [13], nanonofibers [14], nanorods [15] and thin molecular layers [6][7][8][9].…”

Section: Nanostructuresmentioning

confidence: 99%

“…This type of materials can be designed by different molecular ways, i.e. Langmuir-Blodgett (LB) -type techniques [6,7], SAMs or LbL as well as electrolytic deposition [8,9].…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Nanosized Biosensors: Perspectives and Future of Biocatalysts

Sołoducho¹

2013

J Anal Bioanal Tech

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Nano-sized particles in biosensoricsNanosized units have found a large number of applications in biosensorics. Exemplifying, functional nanoparticles can be used AbstractElectrochemical biological sensor device unite the sensitivity of classic instrumental techniques with the inseparable selectivity of the biological agent. The bio-element of the sensing device identifies the analyte following in a biocatalytic way and definitely generates an electrical response recorded by a transducing element. The signal is proportional to concentration of the analyzed substance. Certain of developed modern biosensors are commercial available and are regularly applied in clinical, environmental, or industrial arrangements. The enzymatic electrode is often the principal element of electrochemical biosensors. The choice of suitable composition of agents, in example: biocatalyst, mediators, semiconducting elements, supports, for design of enzymatic sensors directs an electrode activity according to electron transport rate, its stability, and vitality.

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“…Such model molecular assemblies can be prepared by Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) techniques [2,3], layer-by-layer (LbL) or by employing self-assembly monolayers (SAMs) or electrolytic deposition ( Fig. 1) [4,5]. The main advantage of using thin films to build a biosensor is the possibility to decrease dramatically the response time of the device.…”

Section: Introductionmentioning

confidence: 99%