Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará

Silva, Jenevaldo Barbosa da; Lopes, Cinthia Távora de Albuquerque; Souza, Melina Garcia Saraiva de; Gibson, André Felipe Bagarrão; Vinhote, Wagner Marcelo de Souza; Fonseca, Adivaldo Henrique da; Araújo, Flábio R.

doi:10.1590/s0100-736x2014000100002

Cited by 5 publications

(6 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Despite the confirmed presence of this rickettsia in buffalo herds (Silva et al 2014a(Silva et al , 2014b(Silva et al , 2014c(Silva et al , 2015, there is little knowledge about the development of anaplasmosis in this species. Furthermore, there is no research that simultaneously evaluates the pathogenicity of A. marginale in cattle and buffaloes and compares the effects of the disease between the two species.…”

Section: Introductionmentioning

confidence: 99%

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects

et al. 2019

View full text Add to dashboard Cite

The study aimed to evaluate and compare the clinical, laboratory and pathological aspects of buffalo and bovine experimentally infected with AmRio 2 strain of Anaplasma marginale. Four Murrah buffaloes and four crossbred cattle were used in the experiment, which two animals of each species were splenectomized. Strain AmRio 2 of A. marginale was inoculated in all experimental animals. Clinical exams, Packed Cell Volume (PCV), blood counts, blood smears, rickettsemia, necropsy and histopathology were performed in all cases. Semi-Nested-PCR (snPCR) for the msp5 and snPCR for the msp1α target gene for identification of A. marginale in blood samples from animals was done. From positive samples for msp1α snPCR, samples were analyzed for the amino acid sequences of this gene. Two splenectomized cattle presented apathy, pale mucous membranes, jaundice, hyperthermia, and severe anemia. The remaining experimental animals did not show clinical signs. The rickettsemia in all animals was less than 1%. The mean PCV of the splenectomized cattle was below 20% at two-time points after infection. On the blood count, the main changes were observed in splenectomized calves and were characterized by a decrease in red blood cells, hemoglobin, PCV and platelets (p <0.05). All animals presented leukocyte elevation by increased lymphocytes, however, with no significant difference. The average prepatent period was two days in all the animals. The average incubation period in cattle that became ill was 25.5 days, and death occurred, on average, 63 days after inoculation of the strain. The necropsy findings were characterized by pale carcass, ascites, enlarged liver, distended gallbladder, and thick bile. Histopathological findings included infiltration of macrophages and lymphocytes in various organs, hepatic sinusoidal dilatation, and necrosis of the large intestine. In snPCR for the msp5 gene, 100% of the animals were positive in at least one evaluation. And in the snPCR for the infection of the msp1α target gene was also found in all animals in at least one sample evaluated. However, sequencing revealed only five animals, including the bovine which died, with a similarity of the amino acid sequences with AmRio 2 strain of A. marginale. It is concluded that the splenectomized cattle died due to anaplasmosis caused by the inoculated strain and the buffalo were more resistant compared to cattle. Buffaloes can be an alternative to cattle rearing in areas with a high occurrence of clinical cases of anaplasmosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Nas últimas décadas, a bubalinocultura ganhou destaque no cenário nacional, deixando de ser apenas uma alternativa para a ocupação de terras impróprias para a criação de bovinos, e passando a ser uma opção economicamente rentável. Com isso, a preocupação com o manejo sanitário tem aumentado consideravelmente, pois aspectos epidemiológicos, clínicos e patológicos de enfermidades relacionadas a essa espécie, ainda são pouco estudados no país (SILVA et al, 2014).…”

Section: O Introduçãounclassified

Principais Causas De Descarte Em Búfalos Abatidos No Estado Do Rio Grande Do Sul, Brasil

et al. 2016

View full text Add to dashboard Cite

O Brasil possui um rebanho de búfalos com aproximadamente um milhão cento e cinquenta mil cabeças. No estado do Rio Grande do Sul (RS), a bubalinocultura de corte vem crescendo nos últimos anos, principalmente pela precocidade dos animais e pela qualidade da carne. A inspeção post-mortem de órgãos e carcaças é fundamental no processo de triagem de animais e da carne para o consumo humano. Assim, o presente trabalho objetivou analisar e discutir as principais causas de condenação de órgãos e carcaças encontradas durante a inspeção post-mortem de bubalinos em abatedouros sob inspeção estadual no RS, no período entre janeiro e dezembro de 2010. Foram inspecionados 5.614 bubalinos pelas técnicas de exame visual, palpação e incisão de órgãos, realizadas conforme a legislação brasileira. A principal causa de condenação de órgãos foi a congestão pulmonar (10,44%), seguida por nefrite (8,48%). Entre as doenças infecciosas e parasitárias, a mais frequente foi fasciolose (6,16%), seguida pela hidatidose (4,11%), actinobacilose (0,50%), cisticercose (0,43%) e tuberculose (0,02%). Após análise dos resultados, pode-se concluir que fasciolose e hidatidose são importantes causas de condenação no RS. Entretanto, outras causas de condenação devem ser consideradas e estudadas.

show abstract

“…Water buffalo (Bubalus bubalis) are very common in Brazilian Amazonia, where they have been raised for milk and meat because of their high level of rusticity and ability to adapt to flooding regions (VEIGA et al, 1996). Despite being more resistant than cattle to the conditions of the Amazonian region, water buffalo are still susceptible to several infectious and parasitic agents that also affect cattle (MOTA et al, 2002;FERREIRA, 2009;SILVA et al, 2014).…”

mentioning

confidence: 99%

“…Despite being more resistant than cattle to the conditions of the Amazonian region, water buffalo are still susceptible to several infectious and parasitic agents that also affect cattle (MOTA et al, 2002;FERREIRA, 2009;SILVA et al, 2014).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Tuberculosis in water buffalo (Bubalus bubalis) in the Baixo Araguari Region, Amapá, Brazil

et al. 2016

View full text Add to dashboard Cite

Water buffalo are of great economic importance in Brazilian Amazonia, which has the largest herd in Brazil. Bovine tuberculosis caused by Mycobacterium bovis is a zoonotic disease that results in severe losses to water buffalo production. Although the disease has already been described in the country, data on the occurrence and distribution of bovine tuberculosis in water buffalo in Brazil is very scarce. The aim of this study was to determine the frequency of bovine tuberculosis in water buffalo in the Baixo Araguari River region of Amapá, Brazil. Thirty herds, randomly selected from the 41 herds of water buffalo in the region, were sampled. From those herds, 212 randomly selected water buffalo were subjected to the comparative tuberculin skin test. The proportion of Baixo Araguari River region herds that were positive for bovine tuberculosis was 50.0% (95% CI 31.3% to 68.7%) and the proportion of animals that were positive was estimated to be 14.8% (95% CI 7.8% to 21.9%). Our results show that bovine tuberculosis is spread widely among water buffalo in the Baixo Araguari River region, which suggests that measures to control the disease should be undertaken in the region. Key words: Brazilian Amazon. Comparative tuberculin skin test. Epidemiology. Mycobacterium bovis. ResumoA criação de búfalos é de grande importância econômica na Região Amazônica, que abriga o maior rebanho do Brasil. A tuberculose causada pelo Mycobacterium bovis é uma zoonose que leva a perdas severas na criação de búfalos. Apesar de já ter sido descrita no país, dados sobre a ocorrência e distribuição da

show abstract

Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará

Cited by 5 publications

References 18 publications

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects

Experimental infection by Anaplasma marginale in buffaloes and cattle: clinical, hematological, molecular and pathological aspects

Principais Causas De Descarte Em Búfalos Abatidos No Estado Do Rio Grande Do Sul, Brasil

Tuberculosis in water buffalo (Bubalus bubalis) in the Baixo Araguari Region, Amapá, Brazil

Contact Info

Product

Resources

About