Detailed analysis of paternal knockout<i>Grb10</i>mice suggests effects on stability of social behavior, rather than social dominance

Rienecker, Kira D. A.; Chavasse, Alexander T.; Moorwood, Kim; Ward, Andrew; Isles, Anthony R

doi:10.1111/gbb.12571

Cited by 20 publications

(23 citation statements)

References 28 publications

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“…1) in a cross-sectional design. Mice progressed through social hierarchy tests (Rienecker et al, 2019) before the marble burying test, followed by the EPM.…”

Section: Subjectsmentioning

confidence: 99%

“…Animals were weaned and housed as previously described (Rienecker et al, 2019). All groups of mice were housed in environmentally enriched cages (cardboard tubes, shred-mats, chew sticks) of 1-5 adult mice per cage.…”

Section: Subjectsmentioning

confidence: 99%

“…The main measures are "entries to the open arm", and "percent time in open arms". These were reported in Supplementary Information for Rienecker et al, 2019. EPM measures reported here include "total entries", "entries to closed arms", "entries to middle", "velocity", "duration in stretch-attend", and "duration in head dipping behaviour".…”

Section: Elevated Plus Maze (Epm)mentioning

confidence: 99%

“…Imprinted genes, defined by their discrete parent-of-origin monoallelic expression based on epigenetic markers established in the germline (Bartolomei & Ferguson-Smith, 2011), are highly expressed in the brain and are linked to behaviour. In particular, paternal expression of imprinted Grb10 is prominent in monoaminergic regions of the midbrain and is linked to social stability, impulsivity, and risk taking behaviours (Dent et al, 2018;Dent & Isles, 2014;Garfield et al, 2011;Rienecker, Chavasse, Moorwood, Ward, & Isles, 2019). However, many behaviourally and neurobiologically separable constructs have overlapping assessments and manifestation (Isles, Winstanley, & Humby, 2019;Lopez-Guzman, Konova, & Glimcher, 2018;Robbins, Gillan, Smith, de Wit, & Ersche, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, we included the Elevated Plus Maze (EPM), a widely employed test of anxiety (Frye, Petralia, & Rhodes, 2000;Pellow, Chopin, File, & Briley, 1985;Walf & Frye, 2007). Our previous observations of Grb10 +/p colonies show barbering emerges with age (Rienecker et al, 2019). Therefore, our experimental design incorporated age (at 2, 6, and 10 months) to assess whether any potential compulsivity phenotype varied by age.…”

Section: Introductionmentioning

confidence: 99%

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Mice carrying paternal knockout of imprintedGrb10do not show compulsive behaviour

Rienecker

Chavasse

Moorwood

et al. 2020

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Mice lacking paternal expression of imprinted Grb10 show a number of social behaviour deficits, including an enhanced allogrooming phenotype. However, this could also index compulsive behaviour, and the increased whisker barbering seen in Grb10 +/p mice has been suggested to be indicative of a trichotillomania-type behaviour. Here we test whether compulsive behaviour is a more general phenotype in Grb10 +/p mice by examining marble burying at three different adult ages (2, 6 and 10 months). We also examined the mice for potentially confounding anxiety phenotypes using the elevated plus maze (EPM). Grb10 +/p mice showed no difference from wild-type littermate controls on any measure in the marble burying test at any age. There was no difference in standard anxiety measures either, although Grb10 +/p mice displayed more risk-taking behaviours on the EPM than wild-type mice. These data suggest that Grb10 +/p mice are not generally more compulsive, and that the enhanced allogrooming is probably indicative of altered social behaviour. Furthermore, the altered behaviours seen on the EPM adds to other published findings suggesting that Grb10, and imprinted genes more generally, have a role in mediating risk-taking behaviour.

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“…1) in a cross-sectional design. Mice progressed through social hierarchy tests (Rienecker et al, 2019) before the marble burying test, followed by the EPM.…”

Section: Subjectsmentioning

confidence: 99%

Section: Subjectsmentioning

confidence: 99%

Section: Elevated Plus Maze (Epm)mentioning

confidence: 99%