Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment

Abstract

Desulfosporosinus burensis sp. nov., a spore-forming, mesophilic, sulfate-reducing bacterium isolated from a deep clay environment A novel anaerobic, Gram-positive, spore-forming, curved rod-shaped, mesophilic and sulfatereducing bacterium was isolated from pore water collected in a borehole at "490 m in Bure (France). This strain, designated BSREI1 T , grew at temperatures between 5 6C and 30 6C (optimum 25 6C) and at a pH between 6 and 8 (optimum 7). It did not require NaCl for growth, but tolerated it up to 1.5 % NaCl. Sulfate, thiosulfate and elemental sulfur were used as terminal electron acceptors. Strain BSREI1 T used crotonate, formate, lactate, pyruvate, fructose, glycerol and yeast extract as electron donors in the presence of sulfate. The sole quinone was MK-7. The G+C content of the genomic DNA was 43.3 mol%. Strain BSREI1 T had the type strains of Desulfosporosinus lacus (16S rRNA gene sequence similarity of 96.83 %), Desulfosporosinus meridiei (96.31 %) and Desulfosporosinus hippei (96.16 %) as its closest phylogenetic relatives. On the basis of phylogenetic and physiological properties, strain BSREI1 T is proposed as a representative of a novel species of the genus Desulfosporosinus, Desulfosporosinus burensis sp. nov.; the type strain is BSREI1 T (5DSM 24089 T 5JCM 17380 T ).

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“…MAG SbF1 is able to oxidize propionate, lactate, and acetate completely to CO 2 . This was unexpected, since all described species of the genus Desulfosporosinus are so far known as incomplete oxidizers of organic compounds to acetate and CO 2 (56–59). Under butyrate-amended conditions, Desulfosporosinus sp.…”

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