Designing DNAzyme‐Powered Nanomachines Simultaneously Responsive to Multiple MicroRNAs

Yang, Shaojie; Yang, Peng; Du, Huan; Hou, Xiandeng; Chen, Junbo; Zhou, Rongxing

doi:10.1002/chem.201804127

Cited by 17 publications

(9 citation statements)

References 44 publications

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“…To facilitate dynamic DNA assemblies or disassembles, homogenized components are usually used to fabricate amplified or repeated nanodevices. [ 12–17 ] Due to the features of nucleic acids, DNAzymes are often used as components for integration of DNA nanotechnology‐based structures. [ 18–20 ] Compared with traditional proteases, DNAzymes have the advantages of easy construction for point‐of‐care and on‐site assay because of their higher chemical and thermal stability.…”

Section: Introductionmentioning

confidence: 99%

A signal conversion system using binding‐induced strand displacement for disease biomarker assay

Jiang

Yang

et al. 2021

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Using the principle of binding‐induced DNA strand displacement (BINSD), a DNAzyme‐powered nanomachine biosensor for multiple biomarkers via magnetic beads‐based signal conversion was designed. This sensor can convert multiple biomarker recognition into release of predesigned output nucleic acids tagged with streptavidin proteins (SA‐DNA) for activation of DNA nanomachines. In general, we adopted complementary base pairing rules and affinity ligand specific recognition, and three types of signal conversion systems were constructed that realized universal, sensitive, accurate, and specific detection of multiple biomarkers. Taking the advantage of the strong anti‐interference capability of magnetic separation, this strategy could be used for detection of various biomarkers in clinical practice.

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Section: Introductionmentioning

confidence: 99%

A signal conversion system using binding‐induced strand displacement for disease biomarker assay

Jiang

Yang

et al. 2021

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“…It should be noted that the bases on DNAzymes or MNAzymes, except the catalytic core, can be changed for different targets and substrates. So far, a few DNAzyme-/MNAzyme-involved methodologies have been reported for multiple miRNA analysis with fluorescence detection. , The potential of DNAzymes/MNAzymes in multiple miRNA analysis needs further demonstration.…”

Section: Introductionmentioning

confidence: 99%

“…So far, a few DNAzyme-/MNAzyme-involved methodologies have been reported for multiple miRNA analysis with fluorescence detection. 39,40 The potential of DNAzymes/MNAzymes in multiple miRNA analysis needs further demonstration.…”

Section: ■ Introductionmentioning

confidence: 99%

MNAzyme-Catalyzed Amplification Assay with Lanthanide Tags for the Simultaneous Detection of Multiple microRNAs by Inductively Coupled Plasma–Mass Spectrometry

Kang

Chen

et al. 2020

Anal. Chem.

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Quantification of multiple disease-related micro-RNAs (miRNAs) is of great significance for clinical diagnosis. Based on the simultaneous multiple element detection ability of inductively coupled plasma−mass spectrometry (ICP−MS) and good specificity of multicomponent nucleic acid enzymes (MNAzymes), a novel and simple method based on the MNAzyme amplification strategy and lanthanide labeling coupled with ICP− MS detection was proposed for the sensitive and simultaneous detection of three miRNAs . Specifically, a probe consisting of streptavidin-modified magnetic beads (SA-MBs) and three DNA substrates labeled with lanthanide tags ( 159 Tb/ 165 Ho/ 175 Lu) was constructed. In the presence of target miRNAs, three pairs of MNAzymes were assembled where each pair was hybridized with the corresponding miRNA, and then the substrates on the SA-MBs were cleaved by the activated MNAzymes, continuously releasing the fragment with lanthanide tags. The released lanthanide tags in the supernatant were collected after magnetic separation and analyzed by ICP−MS, realizing the simultaneous quantification of multiple miRNAs. The correlation of the lanthanide tag signal with the miRNA concentration fitted well in a linear model in the range of 50−1000 pmol L −1 (miRNA-21) and 50−2000 pmol L −1 (miRNA-155 and miRNA-10b). The limits of detection for three miRNAs were 11−20 pmol L −1 , with the relative standard deviations of 2.2− 2.7%. The recoveries of target miRNAs in the human serum and HepG-2 cells were in the range of 87.2−111% and 93.3−111%, respectively. Overall, the method is ideal for the simultaneous quantification of multiple miRNAs with advantages of low spectral interference, high sensitivity, good selectivity, and strong resistance to the complex matrix.

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“…Therefore, it is not surprising that aberrant miRNA expression is involved in the pathogenesis of many diseases including cardiovascular diseases, neurodegenerative diseases, hematologic diseases, osteologic diseases and cancer. To this day, more than 2,650 different microRNAs have been described in various documents [2][3][4][5][6][7][8][9][10][11][12] .…”

Section: Introductionmentioning

confidence: 99%

Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study

Stejskal¹,

Hlozankova²,

Šigutová³

et al. 2019

Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub

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Background. MicroRNAs (miRNAs) are new generation biomarkers used in oncology, cardiology, metabolic syndrome, obesity or in neurology. miRNAs are short non-coding RNA molecules that regulate gene expression in eukaryotes. Aim. To compare a new commercial method for establishing miRNA (imunoassay) with a commercial kit RT qPCR. Methods. RNA was isolated from whole blood samples obtained from four healthy volunteers. The isolates were liquated and miRNA-93-5p and miRNA-23a-3p were measured independently with commercial hsa-miR-93-5p miREIA and hsa-miR-23a-3p miREIA, and commercial RT-qPCR kits. Results. Both miRNAs had good analytical characteristics, very good correlation with RT qPCR. The results between immunoassay and RT qPCR did not statistically differ. A method based on ELISA was faster (2 h with ELISA vs. 3 h with qPCR) and had lower CV then a method based on RT qPCR (see more text). Conclusion. MicroRNAs from blood or derived fractions are particularly interesting candidates for routine laboratory applications. The immunoassay can be performed on any device that processes the ELISA plates and is therefore available in almost every laboratory.

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Designing DNAzyme‐Powered Nanomachines Simultaneously Responsive to Multiple MicroRNAs

Cited by 17 publications

References 44 publications

A signal conversion system using binding‐induced strand displacement for disease biomarker assay

A signal conversion system using binding‐induced strand displacement for disease biomarker assay

MNAzyme-Catalyzed Amplification Assay with Lanthanide Tags for the Simultaneous Detection of Multiple microRNAs by Inductively Coupled Plasma–Mass Spectrometry

Comparison of a new immunoassay and PCR-based method for quantification of microRNAs in whole blood. A pilot methodical study

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