Designing carbohydrate nanoparticles for prolonged efficacy of antimicrobial peptide

Microencapsulation of nisin in alginate-cellulose nanocrystal (CNC) microbeads for prolonged efficacy against Listeria monocytogenes

“…The BHI-agar deep-well model was adapted from Bi et al (2011a). To prepare the BHI-agar deep-well model for nisin depletion testing, a solution containing BHI solids (3.7 %) and agar (1.0 %) was autoclaved for 20 min at 121°C.…”

“…BHI-agar deep-well model of peptide depletion during storage (left) and bioassay of activity against a pathogen (right), adapted fromBi et al (2011a) …”

Novel Formulations for Antimicrobial Peptides

“…Phytoglycogen (PGG) nanoparticles can carry nisin [227]. PGG is a water-soluble glycogen-like α- d -glucan from plants [228,229].…”

“…PGG is a water-soluble glycogen-like α- d -glucan from plants [228,229]. These novel nisin nanocarriers were prepared from PGG polyssacharide nanoparticles subjected to β-amylolysis and subsequent succinate or octenyl succinate substitution, combined or not with β-dextrin (PGB) [227]. The succinate substitution brings negative charges, and octenyl succinate substitution brings negative charges and hydrophobicity to the nanoparticles [230].…”

“…The surface thinning of nanoparticles due to β-amylolysis resulted in increased nisin loading, leading to prolonged activity of the formulation against L. monocytogenes . The degree of substitution, hydrophobicity, and glucan structure affect nisin loading and release [227]. PGG-based nanoparticles from TEM are shown in Figure 6.…”