Design of microfluidic channel geometries for the control of droplet volume, chemical concentration, and sorting

Tan, Yung Chieh; Fisher, James R.; Lee, Alan I.; Cristini, Vittorio; Lee, Abraham P.

doi:10.1039/b403280m

Cited by 457 publications

(382 citation statements)

References 29 publications

Supporting

Mentioning

371

Contrasting

Unclassified

Order By: Relevance

“…Moreover, drops arriving at a bifurcation will take the path that is dictated by the local streamlines of the carrier fluid. 46 In contrast, the second category is more interesting, from a hydrodynamics point of view, because the flow is strongly modified by capillary effects and by the deformability of the drop interfaces. This places the capillary number based on the velocity of the droplets Ca d ¼ mV d /g at the centre of the discussion.…”

Section: Droplet Transportmentioning

confidence: 99%

Dynamics of microfluidic droplets

2010

View full text Add to dashboard Cite

This critical review discusses the current understanding of the formation, transport, and merging of drops in microfluidics. We focus on the physical ingredients which determine the flow of drops in microchannels and recall classical results of fluid dynamics which help explain the observed behaviour. We begin by introducing the main physical ingredients that differentiate droplet microfluidics from single-phase microfluidics, namely the modifications to the flow and pressure fields that are introduced by the presence of interfacial tension. Then three practical aspects are studied in detail: (i) The formation of drops and the dominant interactions depending on the geometry in which they are formed.(ii) The transport of drops, namely the evaluation of drop velocity, the pressure-velocity relationships, and the flow field induced by the presence of the drop. (iii) The fusion of two drops, including different methods of bridging the liquid film between them which enables their merging.

show abstract

Section: Droplet Transportmentioning

confidence: 99%

Dynamics of microfluidic droplets

2010

View full text Add to dashboard Cite

show abstract

“…Monodisperse droplets with tunable volumes are generated using microfluidic chips with a T-junction shearing zone and droplet size is adjusted by varying channel geometry, flow rate, and dispersed phase viscosity [20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets

et al. 2007

View full text Add to dashboard Cite

RECEIVED DATE (to be automatically inserted after your manuscript is accepted if requiredaccording to the journal that you are submitting your paper to) *colston1@llnl.gov ABSTRACT. The first lab-on-chip system for picoliter droplet generation and PCR amplification with real-time fluorescence detection has performed PCR in isolated droplets at volumes 10 6 smaller than commercial real-time PCR systems. The system utilized a shearing T-junction in a silicon device to generate a stream of monodisperse picoliter droplets that were isolated from the microfluidic channel walls and each other by the oil phase carrier. An off-chip valving system stopped the droplets on-chip, allowing them to be thermal cycled through the PCR protocol without droplet motion. With this system a 10-pL droplet, encapsulating less than one copy of viral genomic DNA through Poisson statistics, showed real-time PCR amplification curves with a cycle threshold of ~18, twenty cycles earlier than 2 commercial instruments. This combination of the established real-time PCR assay with digital microfluidics is ideal for isolating single-copy nucleic acids in a complex environment.

show abstract

“…Through various microchannel geometries, droplet volume can be regulated, chemical concentration of droplets can be adjusted, droplets can be made to undergo fission or fusion, and a series of droplets can be sorted. 58 Combining these geometrical components can be useful for creating a microfluidic device that easily manipulates thousands of reaction vessels.…”

Section: Detection Systems and Devices For Molecular Systems Biologymentioning

confidence: 99%

Microfluidics-based systems biology

2006

View full text Add to dashboard Cite

Systems biology seeks to develop a complete understanding of cellular mechanisms by studying the functions of intra-and inter-cellular molecular interactions that trigger and coordinate cellular events. However, the complexity of biological systems causes accurate and precise systems biology experimentation to be a difficult task. Most biological experimentation focuses on highly detailed investigation of a single signaling mechanism, which lacks the throughput necessary to reconstruct the entirety of the biological system, while high-throughput testing often lacks the fidelity and detail necessary to fully comprehend the mechanisms of signal propagation. Systems biology experimentation, however, can benefit greatly from the progress in the development of microfluidic devices. Microfluidics provides the opportunity to study cells effectively on both a single-and multi-cellular level with high-resolution and localized application of experimental conditions with biomimetic physiological conditions. Additionally, the ability to massively array devices on a chip opens the door for high-throughput, high fidelity experimentation to aid in accurate and precise unraveling of the intertwined signaling systems that compose the inner workings of the cell.

show abstract

Design of microfluidic channel geometries for the control of droplet volume, chemical concentration, and sorting

Cited by 457 publications

References 29 publications

Dynamics of microfluidic droplets

Dynamics of microfluidic droplets

On-Chip, Real-Time, Single-Copy Polymerase Chain Reaction in Picoliter Droplets

Microfluidics-based systems biology

Contact Info

Product

Resources

About