Design of bioluminescent biosensors for assessing contamination of complex matrices

Esimbekova, Elena N.; Kalyabina, Valeriya P.; Kopylova, Kseniya V.; Torgashina, I. G.; Kratasyuk, Valentina A.

doi:10.1016/j.talanta.2021.122509

Cited by 19 publications

(17 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bioluminescent cell-free biosensors have been applied for environmental testing or on-site analysis with portable devices. − We thus sought to reduce the reaction time by replacing RFP with a bioluminescent enzyme (Nluc). Bioluminescence was recorded under lysing conditions using furimazine substrates (50 μM) at 6 h post induction with gold ions.…”

Section: Results and Discussionmentioning

confidence: 99%

Gold-Specific Biosensor for Monitoring Wastewater Using Genetically Engineered Cupriavidus metallidurans CH34

et al. 2021

View full text Add to dashboard Cite

Transcription factor-based whole-cell biosensors have recently become promising alternatives to conventional analytical methods due to their advantage of simplicity, cost-effectiveness, and environmental friendliness. In this study, we used genetic engineering to develop a whole-cell biosensor based on the activation of promoters by CupR via interactions with gold ions, leading to the expression of reporter genes that yield output signals. Altering the promoter sequences was shown to significantly improve the performance of the biosensor strain in terms of gold-specificity. The detection sensitivity of our engineered strains was 42-fold higher than that of wild-type strains. The linear range of the purposed sensor was 125–1000 nM with a limit of detection at 46.5 nM. The effectiveness of the sensor strain was verified in wastewater samples.

show abstract

Section: Results and Discussionmentioning

confidence: 99%

Gold-Specific Biosensor for Monitoring Wastewater Using Genetically Engineered Cupriavidus metallidurans CH34

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Not only do these contaminants pose a risk to human health, but they may also result in economic loss in food processing and availability. 145–147 Therefore, developing effective and efficient strategies for contaminants or biomarkers associated with food quality/safety is imperative. So far, multiple conventional techniques have been developed to achieve this goal, for example, polymerase chain reaction, 148,149 high-performance liquid chromatography, 150,151 gas chromatography-mass spectrometry techniques, 152 etc.…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

Nanozyme-based sensors for detection of food biomarkers: a review

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A classic example is the bioluminescent cellular method based on luminous marine bacteria that has been widely applied since the 1960s [ 27 , 28 , 29 , 30 , 31 ]. The enzymatic bioassay variant was first proposed in 1990 for ecotoxicological monitoring [ 31 ]; extensive utilization since the 1980s has highlighted its numerous advantages [ 32 , 33 , 34 , 35 ]. The primary physicochemical bioluminescent mechanisms and the nature of their interactions in bioluminescent systems have been extensively discussed [ 36 , 37 , 38 , 39 ].…”

Section: Introductionmentioning

confidence: 99%

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

et al. 2022

View full text Add to dashboard Cite

Our current study aimed to adapt a bioluminescent bacteria-based bioassay to monitor the bioeffects of gold nanoparticles (AuNPs). Luminous marine bacteria Photobacterium phosphoreum and AuNPs modified with polyvinylpyrrolidone were employed; low-concentration (≤10−3 g/L) bioeffects of AuNPs were studied. Bioluminescence intensity was used as an indicator of physiological activity in bacteria. Two additional methods were used: reactive oxygen species (ROS) content was estimated with a chemiluminescent luminol method, and bacterial size was monitored using electron microscopy. The bacterial bioluminescent response to AuNPs corresponded to the “hormesis” model and involved time-dependent bioluminescence activation, as well as a pronounced increase in the number of enlarged bacteria. We found negative correlations between the time courses of bioluminescence and the ROS content in bacterial suspensions, demonstrating the relationship between bioluminescence activation and bacterial ROS consumption. The combined effects of AuNPs and a beta-emitting radionuclide, tritium, revealed suppression of bacterial bioluminescent activity (as compared to their individual effects) and a reduced percentage of enlarged bacteria. Therefore, we demonstrated that our bacteria-based bioluminescence assay is an appropriate tool to study the bioeffects of AuNPs; the bioeffects can be further classified within a unified framework for rapid bioassessment.

show abstract

Design of bioluminescent biosensors for assessing contamination of complex matrices

Cited by 19 publications

References 75 publications

Gold-Specific Biosensor for Monitoring Wastewater Using Genetically Engineered Cupriavidus metallidurans CH34

Gold-Specific Biosensor for Monitoring Wastewater Using Genetically Engineered Cupriavidus metallidurans CH34

Nanozyme-based sensors for detection of food biomarkers: a review

Adaptation of a Bacterial Bioluminescent Assay to Monitor Bioeffects of Gold Nanoparticles

Contact Info

Product

Resources

About