Design of a surface-scanning coil detector for direct bacteria detection on food surfaces using a magnetoelastic biosensor

Chai, Yating; Wikle, Howard C.; Wang, Zhenyu; Horikawa, S.; Best, Steve R.; Cheng, Z.-Y.; Dyer, Dave F.; Chin, Bryan A.

doi:10.1063/1.4821025

Cited by 47 publications

(29 citation statements)

References 20 publications

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“…Recently, Chai et al developed a novel ME biosensor measurement technique using a surface scanning coil for practical applications [13][14]38 . In this new method, a planer coil was fabricated and placed closely above the ME sensor.…”

Section: Surface-scanning Coil Detector For Real-time In Situ Pathogementioning

confidence: 99%

“…Recently, magnetostrictive material based biosensors, especially free-standing magnetoelastic (ME) biosensors, have been investigated as a label-free wireless biosensor system for real-time pathogen detection [7][8][9][10][11][12][13][14] . A ME biosensor is comprised of a free-standing sensor platform made of a strip-shaped magnetoelastic particle and a biomolecular recognition element (antibody, phage, enzymes, etc.)…”

Section: Introductionmentioning

confidence: 99%

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High throughput pathogen screening for food safety using magnetoelastic biosensors

Chai

Chin

2015

SPIE Proceedings

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In order to secure food safety, high throughput pathogen screening technique that can quickly identify and isolate unsafe contaminated foods has been long-desired. Recently, magnetoelastic (ME) free-standing biosensors have been investigated as a label-free wireless biosensor system for real-time pathogen detection. ME biosensor is composed of a ME resonator coated with a bio-molecular recognition element that binds specifically with a target pathogen. Once the biosensor comes into contact with the target pathogen, binding occurs, resulting in a decrease of the sensor's resonant frequency. Interrogated through magnetic signals, large amount of ME sensors can be deployed and monitored wirelessly. ME biosensors have been investigated to detect foodborne pathogens in cultures and liquid foods. Recently, it has been demonstrated that phage-based ME biosensors are able to directly detect Salmonella Typhimurium on food surfaces without the requirement of pre-analysis culture preparation. This paper will review the novel ME biosensor technique, including the detection principle, the characterization of the sensor performance, the deployment of multiple sensor detection and their applications, especially for food safety analysis. The ME biosensor technique has the potential to be a powerful pathogen screening tool for detecting contaminated food, identifying critical hazard points, and tracking contamination sources along the entire food supply chain.

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Section: Surface-scanning Coil Detector For Real-time In Situ Pathogementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High throughput pathogen screening for food safety using magnetoelastic biosensors

Chai

Chin

2015

SPIE Proceedings

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“…To form the other measurement sensor for the detection of B. anthracis spores, an ME platform was immobilized with JRB7 phage (5×10 11 vir/ml in 1× TBS) using the same procedure as above. ME biosensors were blocked with BSA to prevent non-specific binding [18][19] . The sensors were immersed in a 1 mg/ml BSA solution for 40 min and washed with deionized water twice 20-21 .…”

Section: E2 Phage and Jrb-7 Phage Immobilization And Bovine Serum Albmentioning

confidence: 99%

In-situ detection of multiple pathogenic bacteria on food surfaces

Chai

Horikawa

et al. 2015

SPIE Proceedings

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Real-time in-situ detection of pathogenic bacteria on fresh food surfaces was accomplished with phage-based magnetoelastic (ME) biosensors. The ME biosensor is constructed of a small rectangular strip of ME material that is coated with a biomolecular recognition element (phage, antibodies or proteins, etc.) that is specific to the target pathogen. This mass-sensitive ME biosensor is wirelessly actuated into mechanical resonance by an externally applied time-varying magnetic field. When the biosensor binds with target bacteria, the mass of the sensor increases, resulting in a decrease in the sensor's resonant frequency. In order to compensate for nonspecific binding, control biosensors without phage were used in this experiment. In previous research, the biosensors were measured one by one. However, the simultaneous measurement of multiple sensors was accomplished in this research, and promises to greatly shorten the analysis time for bacterial detection. Additionally, the use of multiple biosensors enables the possibility of simultaneous detection of different pathogenic bacteria. This paper presents results of experiments in which multiple phage-based ME biosensors were simultaneously monitored. The E2 phage and JRB7 phage from a landscape phage library served as the bio-recognition element that have the capability of binding specifically with Salmonella typhimurium and B. anthracis spores, respectively. Real-time in-situ detection of Salmonella typhimurium and B. anthracis spores on food surfaces are presented.

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“…Phages have been used in a magnetoelastic biosensor to detect Salmonella on the surfaces of watermelons,47 tomatoes,48 and egg shells49 and in milk 50. The phages are coated onto the biosensor chip, and when Salmonella cells bind to the phages the mass of the biosensor increases.…”

Section: Immobilized Phage-based Devicesmentioning

confidence: 99%

Prevention of bacterial foodborne disease using nanobiotechnology

Billington¹,

Hudson²,

D'Sa³

2014

NSA

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Foodborne disease is an important source of expense, morbidity, and mortality for society. Detection and control constitute significant components of the overall management of foodborne bacterial pathogens, and this review focuses on the use of nanosized biological entities and molecules to achieve these goals. There is an emphasis on the use of organisms called bacteriophages (phages: viruses that infect bacteria), which are increasingly being used in pathogen detection and biocontrol applications. Detection of pathogens in foods by conventional techniques is time-consuming and expensive, although it can also be sensitive and accurate. Nanobiotechnology is being used to decrease detection times and cost through the development of biosensors, exploiting specific cell-recognition properties of antibodies and phage proteins. Although sensitivity per test can be excellent (eg, the detection of one cell), the very small volumes tested mean that sensitivity per sample is less compelling. An ideal detection method needs to be inexpensive, sensitive, and accurate, but no approach yet achieves all three. For nanobiotechnology to displace existing methods (culture-based, antibody-based rapid methods, or those that detect amplified nucleic acid) it will need to focus on improving sensitivity. Although manufactured nonbiological nanoparticles have been used to kill bacterial cells, nanosized organisms called phages are increasingly finding favor in food safety applications. Phages are amenable to protein and nucleic acid labeling, and can be very specific, and the typical large “burst size” resulting from phage amplification can be harnessed to produce a rapid increase in signal to facilitate detection. There are now several commercially available phages for pathogen control, and many reports in the literature demonstrate efficacy against a number of foodborne pathogens on diverse foods. As a method for control of pathogens, nanobiotechnology is therefore flourishing.

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Design of a surface-scanning coil detector for direct bacteria detection on food surfaces using a magnetoelastic biosensor

Cited by 47 publications

References 20 publications

High throughput pathogen screening for food safety using magnetoelastic biosensors

High throughput pathogen screening for food safety using magnetoelastic biosensors

In-situ detection of multiple pathogenic bacteria on food surfaces

Prevention of bacterial foodborne disease using nanobiotechnology

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