Design and testing of aptamer-based electrochemical biosensors for proteins and small molecules

Cheng, Alan K. H.; Sen, Dipankar; Yu, Hua-Zhong

doi:10.1016/j.bioelechem.2009.04.007

Cited by 144 publications

(78 citation statements)

References 65 publications

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“…The first G4 electrochemical aptasensors used TBA sequences and gold electrodes as electrochemical transducers, the aptamers' attachment being achieved using an amine or a thiol functionalization [50][51][52], or the affinity of biotin to avidin, streptavidin or neutravidin [53]. Depending on the assay format, two main G4 electrochemical aptasensor categories can be depicted, the sandwich-type aptasensors (also named dual-site binding) and the structure switching-based aptasensors (single-site binding) [54,55].…”

Section: G4 Electrochemical Aptasensorsmentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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Guanine-rich nucleic acids are able to self-assemble into G-quadruplex four-stranded secondary structures, which are found at the level of telomeric regions of chromosomes, oncogene promoter sequences and other biologically-relevant regions of the genome. Due to their extraordinary stiffness and biological role, G-quadruples become relevant in areas ranging from structural biology to medicinal chemistry, supra-molecular chemistry, nanotechnology and biosensor technology. In addition to classical methodologies, such as circular dichroism, nuclear magnetic resonance or crystallography, electrochemical methods have been successfully used for the rapid detection of the conformational changes from single-strand to G-quadruplex. This review presents recent advances on the G-quadruplex electrochemical characterization and on the design and applications of G-quadruplex electrochemical biosensors, with special emphasis on the G-quadruplex aptasensors and hemin/G-quadruplex peroxidase-mimicking DNAzyme biosensors.

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Section: G4 Electrochemical Aptasensorsmentioning

confidence: 99%

Guanine Quadruplex Electrochemical Aptasensors

Chiorcea-Paquim

Oliveira-Brett

2016

Chemosensors

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“…The majority of G-quadruplex electrochemical aptasensors are using thrombin binding aptamers and gold electrodes as electrochemical transducers, the aptamers attachment being achieved using amine or thiol functionalization [45][46][47]48] or the affinity of biotin to avidin, streptavidin or neutravidin [49,50].…”

Section: G-quadruplex Electrochemical Aptasensorsmentioning

confidence: 99%

Redox behaviour of G-quadruplexes

Chiorcea-Paquim

Oliveira-Brett

2014

Electrochimica Acta

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Please cite this article as: A.-M. Chiorcea-Paquim, A.M. OliveiraBrett, Redox behaviour of G-quadruplexes, Electrochimica Acta (2013), http://dx.doi.org/10.1016/j.electacta. 2013.07.150 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Page 1 of 45A c c e p t e d M a n u s c r i p t Redox behaviour of G-quadruplexes

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“…The binding of analyte to aptamer is monitored by measuring current, potential or impedance. The main advantage of this approach lies in the possibility of label-free detection (Mairal et al 2008;Cheng et al 2009;Ferapontova and Gothelf 2011). Optical aptasensors utilize aptamers labeled with optical probes (for example, fluorescence) or are attached to optically active nanoparticles.…”

Section: Dna Aptamers Containing G-quadruplexes In Biosensorsmentioning

confidence: 99%

Potential uses of G-quadruplex-forming aptamers

Víglaský

Hianik

2013

gpb

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Abstract. Guanine quadruplex (G-quadruplex) structures are one of a number of structures which are capable of adopting aptamers. G-rich DNA or RNA has an increased propensity to form quadruplex structures which have unusual biophysical and biological properties. G-rich aptamers which form G-quadruplexes have several advantages over unstructured sequences: G-quadruplexes are non-immunogenic, thermodynamically and chemically stable and they have both higher resistance to various serum nucleases and an enhanced cellular uptake. These advantages have led to a number of synthetic oligonucleotides being studied for their potential use as therapeutic agents for cancer therapy and in the treatment of various other diseases. In addition to their suitability in the fields of medicine and biotechnology, these, highly specified, aptameric G-quadruplexes also have great potential in the further development of nano-devices; e.g. basic components in microarrays, microfluidics, sandwich assays and electrochemical biosensors. This review summarizes the biophysical properties of G-quadruplexes and highlights the importance of the stability and recognition properties of aptamers. Examples of the application of aptamers in medical therapy and in biosensors are also discussed.

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Design and testing of aptamer-based electrochemical biosensors for proteins and small molecules

Cited by 144 publications

References 65 publications

Guanine Quadruplex Electrochemical Aptasensors

Guanine Quadruplex Electrochemical Aptasensors

Redox behaviour of G-quadruplexes

Potential uses of G-quadruplex-forming aptamers

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