Desenvolvimento de métodos cromatográficos para análises de antimicrobianos em amostras complexas

Melo, Lidervan de Paula

doi:10.11606/t.59.2012.tde-17072012-142923

Cited by 1 publication

(1 citation statement)

References 105 publications

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“…In this study, we examined the efficacy of a PCR-based method to diagnose the presence of S. mansoni eggs in the stool samples of individuals with low-intensity infections. To adequately monitor and control schistosomiasis, more accurate diagnostic methods are needed, which is especially pressing due to the high prevalence and incidence of this disease in some regions ( Melo 2006 ). The control of schistosomiasis is now possible due to the development of a single-dose oral medication that can be easily administered to infected patients.…”

Section: Discussionmentioning

confidence: 99%

A conventional polymerase chain reaction-based method for the diagnosis of human schistosomiasis in stool samples from individuals in a low-endemicity area

Carneiro

Peralta

Pinheiro

et al. 2013

Mem. Inst. Oswaldo Cruz

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The aim of this study was to evaluate the efficacy of a polymerase chain reaction (PCR)-based method to detect Schistosoma mansoni DNA in stool samples from individuals living in a low-endemicity area in Brazil. Of the 125 initial stool samples, 80 were ELISA reactive and eggs were identified in 19 of the samples by parasitological examination. For the PCR evaluations, 56 stool samples were selected and divided into five groups. Groups I-IV were scored negative for S. mansoni eggs by parasitological examination. Groups I and II were ELISA reactive, whereas Groups III and IV were ELISA nonreactive. Groups II and III were positive for other intestinal parasites. PCR testing scored eight samples as positive from these four groups. Group V represented the S. mansoni -positive group and it included ELISA-reactive samples that were scored positive for S. mansoni by one or more parasitological examinations (6/19 were positive by Kato-Katz method, 9/17 by saline gradient and 10/13 by Helmintex®). PCR scored 13 of these 19 samples as positive for S. mansoni . We conclude that while none of these methods yielded 100% sensitivity, a combination of techniques should be effective for improving the detection of S. mansoni infection in low-endemicity areas.

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Section: Discussionmentioning

confidence: 99%