Description of two novel members of the family Erysipelotrichaceae: Ileibacterium valens gen. nov., sp. nov. and Dubosiella newyorkensis, gen. nov., sp. nov., from the murine intestine, and emendation to the description of Faecalibacterium rodentium

Abstract

To better characterize murine intestinal microbiota, a large number (187) of Gram-positive-staining, rod- and coccoid-shaped, and facultatively or strictly anaerobic bacteria were isolated from small and large intestinal contents from mice. Based on 16S rRNA gene sequencing, a total 115 isolates formed three phylogenetically distinct clusters located within the family Erysipelotrichaceae. Group 1, as represented by strain NYU-BL-A3T, was most closely related to Allobaculum stercoricanis, with 16S rRNA gene sequence similarity values of 87.7 %. A second group, represented by NYU-BL-A4T, was most closely related to Faecalibaculum rodentium, with 86.6 % 16S rRNA gene sequence similarity. A third group had a nearly identical 16S rRNA gene sequence (99.9 %) compared with the recently described Faecalibaculum rodentium, also recovered from a laboratory mouse; however, this strain had a few differences in biochemical characteristics, which are detailed in an emended description. The predominant (>10 %) cellular fatty acids of strain NYU-BL-A3T were C16 : 0 and C18 : 0, and those of strain NYU-BL-A4T were C10 : 0, C16 : 0, C18 : 0 and C18 : 1ω9c. The two groups could also be distinguished by multiple biochemical reactions, with the group represented by NYU-BL-A4T being considerably more active. Based on phylogenetic, biochemical and chemotaxonomic criteria, two novel genera are proposed, Ileibacterium valens gen. nov., sp. nov. with NYU-BL-A3T (=ATCC TSD-63T=DSM 103668T) as the type strain and Dubosiella newyorkensis gen. nov., sp. nov. with NYU-BL-A4T (=ATCC TSD-64T=DSM 103457T) as the type strain.

Assembly of 913 microbial genomes from metagenomic sequencing of the cow rumen

“…Of the RUGs that checkM could only resolve to the Bacteria kingdom, we noticed that 31 of them had significant numbers of protein hits to the Solobacterium , Coprobacillus and Kandleria genera, which are all members of the Erysipelotrichales order. Members of the Erysipelotrichales have been isolated from the human 11 , mouse 46 and insect gut 47 and pig manure 48 . We hypothesized that some of the 31 RUGs may represent new clades within Erysipelotrichales .…”

Inulin Exerts Beneficial Effects on Non-Alcoholic Fatty Liver Disease via Modulating gut Microbiome and Suppressing the Lipopolysaccharide-Toll-Like Receptor 4-Mψ-Nuclear Factor-κB-Nod-Like Receptor Protein 3 Pathway via gut-Liver Axis in Mice

“…Importantly, this abnormal increased Blautia could be rectified with INU supplementation, suggesting that suppression of Blautia may partially contribute to the effectiveness of INU on NAFLD. Ileibacterium , a novel member of the Allobaculum genus found in 2017, was associated with metabolism exerting a vigorous response to dietary changes, eventually showing protective potential for obesity ( Cox et al, 2017 ; den Hartigh et al, 2018 ). We also found that an enrichment of Ileibacterium with HFD administration.…”

Associations of NOD2 polymorphisms with Erysipelotrichaceae in stool of in healthy first degree relatives of Crohn’s disease subjects

“…Also, this taxon was found to be highly coated by IgA relative to other members of the gut microbiota in IBD patients [26]. It includes strictly anaerobic or aerotolerant organisms, and members of the Erysipelotrichaceae family can produce palmitic, stearic and oleic acid, fatty acids predominantly [27]. Over accumulation of tissue palmitic acid results in dyslipidemia, hyperglycemia, increased ectopic fat accumulation, increased inflammatory tone via toll-like receptor 4 [28] as well as pro-inflammatory adipokines mRNA expression in macrophages [29].…”