Description of Aedes (Hulecoeteomyia) bhutanensis n. sp. (Diptera: Culicidae) from Bhutan

Reconsideration of the status of subspecies in the Japonicus Group of the subgenus Hulecoeteomyia Theobald of Aedes Meigen (Diptera: Culicidae)

“…In comparison to Ae. bhutanensis, Somboon et al (2020) found that the CoI mtDNA sequences of amamiensis and yaeyamensis were distinct from those of japonicus s.s., differing by 43 (7.4%) and 49 (8.4%) fixed sites, respectively. In the same study, Ae.…”

“…(Hul.) bhutanensis Somboon & Harbach, 2020(in Somboon et al 2020, was recently added. Tanaka et al (1979) defined the Japonicus Group as follows: "Aedeagus broadly open in tergobasal 0.67 and sternoapical 0.75, without apical denticle, slightly constricted in apical 0.33.…”

Aedes yunnanensis (Diptera: Culicidae): transferred from the subgenus Hulecoeteomyia to Orohylomyia subg. nov., with descriptions of the species and the new subgenus

“…A number of adult and larval specimens were identified by DNA sequencing and phylogenetic analyses of COI sequences, as reported by Somboon et al (2020). Two legs (fore- and midleg) of adults and abdominal segment VI of larvae were used for DNA extraction.…”

“…Following Somboon et al (2020), DNA was extracted using Gene JET FFPE DNA Purification Kit (Thermo Fisher Scientific, USA) following the manufacturer's protocol. The mitochondrial cytochrome c oxidase subunit I ( COI ) gene was amplified using the barcoding primers LCO1490 (5′-GGT CAA CAA ATC ATA AAG ATA TTG G-3′) and HCO2198 (5′-TAA ACT TCA GGG TGA CCA AAA AAT CA-3′) of Folmer et al (1994).…”

Evidence for the spread of the alien species Aedes koreicus in the Lombardy region, Italy

“…japonicus sequence (GenBank: MG242580.1) with 98.3% bootstrap support. Indeed, the three species are recognized members of the Hulecoeteomyia subgenus [ 24 ].…”