Descripción del ARN de transferencia mitocondrial para Serina (UCN) de Lutzomyia columbiana (Diptera, Psychodidae)

Pérez-Doria, Alveiro; Bejarano, Eduar Elías; Vélez, Iván Darío

doi:10.1590/s0085-56262008000400008

Cited by 3 publications

(2 citation statements)

References 24 publications

(16 reference statements)

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“…The differences between the sequences were represented mainly by transitions; this could be caused by a deficiency in the repair mechanisms of mitochondrial DNA (Ruiz et al, 2008). The nucleotide composition in the sequences for the three tRNA was dominated by adenine (~38%), cytosine (~27%) and thymine (~20%), only between 15 and 18% corresponded to a guanine for all sequences, as was expected in a mitochondrial gene (Pérez, Bejarano & Vélez, 2008). This fact reflects that tRNA Trp , tRNA Lys and tRNA Leu(CUN) are light tRNA, that is, are transcribed from the heavy chain (Helm et al, 2000).…”

Section: Discussionmentioning

confidence: 74%

Structural analysis of leucine, lysine and tryptophan mitochondrial tRNA of nesting turtles Caretta caretta (Testudines: Chelonioidea) in the Colombian Caribbean

Infante-Rojas

Mariño‐Ramírez

Fernández

2020

PeerJ

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The understanding of the functional properties of mitochondrial transfer RNA (mt tRNAs) depend on the knowledge of its structure. tRNA acts as an interface between polynucleotides and polypeptides thus, they are key molecules in protein biosynthesis. The tRNA molecule has a functional design and, given its importance in the translation of mitochondrial genes, it is plausible that modifications of the structure can affect the synthesis of proteins and the functional properties of the mitochondria. In a previous work, the mitochondrial genome of an individual of the nesting Caretta caretta of the Colombian Caribbean was obtained, where specific mutations were identified in the only tRNALeu (CUN), tRNATrp and tRNALys genes. In order to analyze the effect of these mutations on these three mt tRNAs, the prediction of 2D and 3D structures was performed. Genes were sequenced in 11 nesting loggerhead turtles from the Colombian Caribbean. Two-dimensional structures were inferred using the ARWEN program, and three-dimensional structures were obtained with the RNA Composer 3D program. Two polymorphisms were identified in tRNATrp and another one was located in tRNALys, both specific to C. caretta. The thymine substitution in nucleotide position 14 of tRNATrp could constitute an endemic polymorphism of the nesting colony of the Colombian Caribbean. Two 2D and three 3D patterns were obtained for tRNATrp. In the case of tRNALys and tRNALeu 2D and 3D structures were obtained respectively, which showed compliance to canonical structures, with 4 bp in the D-arm, 4–5 bp in the T-arm, and 5 bp in the anticodon arm. Moderate deviations were found, such as a change in the number of nucleotides, elongation in loops or stems and non-Watson–Crick base pairing: adenine–adenine in stem D of tRNATrp, uracil–uracil and adenine–cytosine in the acceptor arm of the tRNALys and cytosine–cytosine in the anticodon stem of the tRNALeu. In addition, distortions or lack of typical interactions in 3D structures gave them unique characteristics. According to the size of the variable region (4–5 nt), the three analyzed tRNAs belong to class I. The interactions in the three studied tRNAs occur mainly between D loop—variable region, and between spacer bases—variable region, which classifies them as tRNA of typology II. The polymorphisms and structural changes described can, apparently, be post-transcriptionally stabilized. It will be crucial to perform studies at the population and functional levels to elucidate the synthetic pathways affected by these genes. This article analyses for the first time the 1D, 2D and 3D structures of the mitochondrial tRNALys, tRNATrp and tRNALeu in the loggerhead turtle.

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“…Recently, the mitochondrial transfer gene for serine (UCN) has been proposed as a molecular marker for the genetic characterization and diagnosis of the species (Vivero et al 2007, Pérez-Doria 2008, Pérez-Doria 2011, being explored in 11 species of phlebotomine with emphasis on species with importance in the transmission of leishmaniosis. In the present article we describe, for the first time, the primary and secondary structure of the mitochondrial transfer RNA for serine that recognizes the codon UCN (tRNASer) of L. longipalpis from different localities to differentiate it from other species of the subgenus Lutzomyia.…”

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Prediction of the secundary structure at the tRNASer (UCN) ofLutzomyia longipalpis(Diptera: Psychodidae)

Hoyos-López

2018

Preprint

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Abstract. Lutzomyia longipalpis is the main vector of Leishmania infantum, the etiological agent of visceral leishmaniasis in America and Colombia. Taxonomically belongs to the subgenusLutzomyia, which includes other vector species that exhibit high morphological similarity to the female species difficult to identify vectors in leishmaniasis foci and suggesting the search for molecular markers that facilitate this task, further researchs with mitochondrial genes, chromosome banding, reproductive isolation and pheromones evidence the existence of species complex. The aim of this study was to predict the secondary structure of mitochondrial transfer RNA serine (tRNASer) for UCN codon of Lutzomyia longipalpis as molecular marker for identify of this species. Sequences recorded in Genbank of L. longipalpis sequences were aligned with tRNA's from previously described species and then tRNASer secondary structure was inferred by software tRNAscan-SE 1.21. The length of tRNASer was 67 base pairs (bp). Two haplotypes were detected in the five sequences analyzed. The L. longipalpis tRNASer showed 7 intrachain pairing in the acceptor arm, 3 in the DHU arm, 4 in the anticodon arm and 5 in the TψC. The size of the loops corresponded to 5 nucleotides in the DHU, 7 in the anticodon, 4 in the variable and 7 in the TψC. L. longipalpis is distinguished from other species at subgenera . CC-BY-NC-ND 4.0 International license peer-reviewed) is the author/funder. It is made available under a The copyright holder for this preprint (which was not . http://dx.doi.org/10.1101/261297 doi: bioRxiv preprint first posted online Feb. 7, 2018; Lutzomyia by the secondary structure and substitutions inferred tRNASer evidenced in the primary sequence.

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Descripción del ARN de transferencia mitocondrial para Serina (UCN) de Lutzomyia columbiana (Diptera, Psychodidae)

Cited by 3 publications

References 24 publications

Structural analysis of leucine, lysine and tryptophan mitochondrial tRNA of nesting turtles Caretta caretta (Testudines: Chelonioidea) in the Colombian Caribbean

Prediction of the secundary structure at the tRNASer (UCN) ofLutzomyia longipalpis(Diptera: Psychodidae)

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