Dermal Drivers of Injury-Induced Inflammation: Contribution of Adipocytes and Fibroblasts

Cooper, Paula O.; Haas, MaryEllen R; Noonepalle, Satish; Shook, Brett A.

doi:10.3390/ijms22041933

Cited by 29 publications

(19 citation statements)

References 241 publications

(381 reference statements)

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“…Both bacteria and endotoxins elevate proinflammatory cytokines such as TNF- α and interleukin-1. Furthermore, inflammation increases protease enzyme in the tissue which degrades growth factors rapidly [ 26 , 27 ]. If this situation continues in the wound, it leads to failure in healing .…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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Estrogens are a group of sex hormones which have receptors on the skin and lead to increased cells and wound healing. Normally isoflavonoids are present in Astragalus floccosus Boiss. (Leguminosae). Therefore, the present study was conducted to evaluate the presence of isoflavonoids in A. floccosus’ rich fraction of flavonoid and evaluate its wound healing effect accordingly. Flavonoids were evaluated by LCMS. Scratch was conducted and the medium culture was treated with the Astragalus’ rich fraction of flavonoid (RFF) and was compared with nontreated culture during 48 hours. In addition, in vivo full-thickness wound healing evaluation was performed on rats. The rats were put into four groups and treated on a daily basis for 21 days with a cream containing 1.5% of the RFF (group 1), silver sulfadiazine (group 2), and Vaseline (group 3) separately. The nontreated group (group 4) was created for a better comparison. During the examination, wound size was evaluated and histopathological examination was performed. Herbal analysis detected 11 flavonoids, including 2 isoflavonoids, Calycosin-7-O-beta-D-glucoside and Formononetin, in the RFF. In vitro scratch wound healing showed significant improvement with RFF treatment in comparison to nontreated medium. Furthermore, in vitro drug release of Astragalus ointment showed a stationary line during 24 h and 0.14 mg/ml of flavonoid penetrated the skin. In vivo wound size evaluation showed significant improvement in the group treated with the RFF in comparison to other groups. Histopathological results indicated that congestion, edema, inflammation, necrosis, and angiogenesis decreased during the examination and fibroblast proliferation fibrosis epithelization was increased especially in the RFF group in comparison to the silver sulfadiazine and free groups. In conclusion, A. floccosus showed that wound healing activity in both in vitro and in vivo analyses can be attributed to the presence of isoflavonoids with estrogen-like activity in this plant.

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Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Akbari

Azadbakht

Bagheri

et al. 2022

Advances in Pharmacological and Pharmaceutical Sciences

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“…Fibroblasts represent a very heterogeneous and plastic cell population regulating both the homeostasis of connective tissue and the inflammatory process and being involved in pathological conditions [ 80 , 105 , 197 , 198 ]. In particular, fibroblast populations are able to shift from an inactivated phenotype of quiescent fibroblasts to an activated phenotype of myofibroblasts or a constitutively activated phenotype of MAFs, depending on environmental modifications and cellular interactions [ 2 , 80 ].…”

Section: Discussionmentioning

confidence: 99%

Influence of Tumor Microenvironment and Fibroblast Population Plasticity on Melanoma Growth, Therapy Resistance and Immunoescape

Romano¹,

Belviso²,

Venuta³

et al. 2021

IJMS

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Cutaneous melanoma (CM) tissue represents a network constituted by cancer cells and tumor microenvironment (TME). A key feature of CM is the high structural and cellular plasticity of TME, allowing its evolution with disease and adaptation to cancer cell and environmental alterations. In particular, during melanoma development and progression each component of TME by interacting with each other and with cancer cells is subjected to dramatic structural and cellular modifications. These alterations affect extracellular matrix (ECM) remodelling, phenotypic profile of stromal cells, cancer growth and therapeutic response. The stromal fibroblast populations of the TME include normal fibroblasts and melanoma-associated fibroblasts (MAFs) that are highly abundant and flexible cell types interacting with melanoma and stromal cells and differently influencing CM outcomes. The shift from the normal microenvironment to TME and from normal fibroblasts to MAFs deeply sustains CM growth. Hence, in this article we review the features of the normal microenvironment and TME and describe the phenotypic plasticity of normal dermal fibroblasts and MAFs, highlighting their roles in normal skin homeostasis and TME regulation. Moreover, we discuss the influence of MAFs and their secretory profiles on TME remodelling, melanoma progression, targeted therapy resistance and immunosurveillance, highlighting the cellular interactions, the signalling pathways and molecules involved in these processes.

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“…Under the activation of IL-α released by keratinocytes and TLR ligands in the wound microenvironment, fibroblasts activate the NF-kB pathway for the induction and recruitment of inflammatory cells by proinflammatory cytokines (TNF α, INF γ, IL-6, IL-8), chemokines (CCL1, CCL2, CCL5, CXCL1, CXCL8, and CXCL10), and growth factors (granulocyte/macrophage colony stimulating factor (GM-CSF) and granulocyte colony stimulating factor (G-CSF) ( 4 , 9 , 90 , 91 ). CCL2 is closely associated with monocyte recruitment ( 92 ). Like keratinocytes, fibroblasts also synthesise AMPs and defensins (hBD-1 and hBD-2) to clean wounds ( 9 , 93 ).…”

Section: Role Of Non-inflammatory Cells In the Inflammatory Microenvi...mentioning

confidence: 99%

“…A large number of pro-inflammatory factors increased the release of MMPs and accelerated the degradation of the ECM, which further reduced the proliferation and collagen deposition of fibroblasts ( 4 , 166 ). In this pathological inflammatory microenvironment, inflammatory genes expressed by fibroblasts are up-regulated and keratinocytes release IL-1α and type I interferon, triggering an inflammatory chain reaction in adjacent stromal cells ( 92 , 162 ). This, in turn, promotes the flow of immune cells into the injured site.…”

Section: Cell-cell and Cell-ecm Interactionsmentioning

confidence: 99%

Inflammatory Microenvironment of Skin Wounds

et al. 2022

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Wound healing is a dynamic and highly regulated process that can be separated into three overlapping and interdependent phases: inflammation, proliferation, and remodelling. This review focuses on the inflammation stage, as it is the key stage of wound healing and plays a vital role in the local immune response and determines the progression of wound healing. Inflammatory cells, the main effector cells of the inflammatory response, have been widely studied, but little attention has been paid to the immunomodulatory effects of wound healing in non-inflammatory cells and the extracellular matrix. In this review, we attempt to deepen our understanding of the wound-healing microenvironment in the inflammatory stage by focusing on the interactions between cells and the extracellular matrix, as well as their role in regulating the immune response during the inflammatory stage. We hope our findings will provide new ideas for promoting tissue regeneration through immune regulation.

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Dermal Drivers of Injury-Induced Inflammation: Contribution of Adipocytes and Fibroblasts

Cited by 29 publications

References 241 publications

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae)

Influence of Tumor Microenvironment and Fibroblast Population Plasticity on Melanoma Growth, Therapy Resistance and Immunoescape

Inflammatory Microenvironment of Skin Wounds

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