Derivation of Human Microvascular Endothelial Cells for Prosthetic Vascular Graft Seeding

Sharp, William V.; Schmidt, Steven; Meerbaum, Sharon O.; Pippert, Todd R.

doi:10.1016/s0890-5096(06)62001-3

Cited by 18 publications

(11 citation statements)

References 9 publications

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“…Based on cell surface expression of CD31 and HLA DR, intracellular expression of vWF, and very high expression of mRNAs typical of ECs, the CD31 + subset of SVF cells was considered to consist of ECs, most likely microvascular ECs [29]. Previously, several attempts had been made to isolate ECs from SVF using plastic attachment techniques and positive selection strategies [44–48]. We recently used a combination of negative and positive immunomagnetic isolation to derive a pure population of CD31 + cells from AT [29].…”

Section: Endothelial Cells In Adipose Tissuementioning

confidence: 99%

Concise Review: Therapeutic Potential of Adipose Tissue-Derived Angiogenic Cells

Szőke

Brinchmann

2012

Stem Cells Translational Medicine

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Inadequate blood supply to tissues is a leading cause of morbidity and mortality today. Ischemic symptoms caused by obstruction of arterioles and capillaries are currently not treatable by vessel replacement or dilatation procedures. Therapeutic angiogenesis, the treatment of tissue ischemia by promoting the proliferation of new blood vessels, has recently emerged as one of the most promising therapies. Neovascularization is most often attempted by introduction of angiogenic cells from different sources. Emerging evidence suggests that adipose tissue (AT) is an excellent reservoir of autologous cells with angiogenic potential. AT yields two cell populations of importance for neovascularization: AT-derived mesenchymal stromal cells, which likely act predominantly as pericytes, and AT-derived endothelial cells (ECs). In this concise review we discuss different physiological aspects of neovascularization, briefly present cells isolated from the blood and bone marrow with EC properties, and then discuss isolation and cell culture strategies, phenotype, functional capabilities, and possible therapeutic applications of angiogenic cells obtained from AT. STEM CELLS TRANS- LATIONAL MEDICINE 2012;1:658 -667

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Section: Endothelial Cells In Adipose Tissuementioning

confidence: 99%

Concise Review: Therapeutic Potential of Adipose Tissue-Derived Angiogenic Cells

Szőke

Brinchmann

2012

Stem Cells Translational Medicine

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“…This is based on in vitro studies with mesothelial cell seedings on vascular grafts which showed low thrombogenicity (Pronk et al, 1988). Mesothelial cells produce prostacyclin and they have fibrinolytic activity in the same way as endothelial cells (Kent et al, 1988;Sharp et al, 1989;Deutsch et al, 1999;Maeda et al, 2000). Another reason for the different patency results can be an improper anticoagulant therapy.…”

Section: Discussionmentioning

confidence: 99%

Morphological evaluation of experimental autologous rectus fascia sheath vascular grafts used for arterial replacement in a dog model

Csébi

Jakab

Patonai

et al. 2014

Acta Veterinaria Hungarica

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Although experimental autologous patch or tubular conduit vascular grafts made from the internal rectus fascia sheath (IRFS) have been reported in the literature, thorough morphological evaluation and verification of the histological arterialisation of such grafts are lacking. Four purpose-bred Beagle dogs were utilised to create eight arterial internal rectus fascia sheath (ARFS) grafts implanted between bisected ends of the external iliac arteries. Four out of the eight ARFS grafts were patent after three months. Haematoxylin-eosin and Azan staining verified that the grafts gained a vessel-like layered structure with the presence of large amounts of collagen fibres. Although the inner surface of the intact IRFS was originally covered with claudin-5-negative and pancytokeratin-positive mesothelial cells in control samples, the internal cells of the ARFS grafts became claudin-5 positive and pancytokeratin negative like in intact arteries. Spindle-shaped cells of the wall of ARFS grafts were α-smooth muscle actin (α-SMA) positive just like the smooth muscle cells of intact arteries, but α-SMA immunoreactivity was negative in the intact IRFS. According to these findings, the fibroblast cells of the ARFS graft have changed into myofibroblast cells. The study has proved that ARFS grafts may be used as an alternative in arterial replacement, since the graft becomes morphologically and functionally similar to the host vessel via arterialisation.Key words: Rectus fascia sheath, autologous vascular graft, immunohistochemistry, claudin-5, alpha-SMA, desmin, pancytokeratin, veterinary field Several different types of vascular substitutes are used in vascular surgery including synthetic vascular prostheses, autologous vessels, heterologous donor

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“…Mesothelial cells produce prostacyclin and they have fibrinolytic activity in the same way as endothelial cells. This means that intact and original endothelial or mesothelial cells present an ideal situation in all cases (Kent et al, 1988;Sharp et al, 1989;Deutsch et al, 1999;Maeda et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Experimental results and clinical impact of using autologous rectus fascia sheath for vascular replacement

Kopper

Németh

Nagy

et al. 2008

Acta Veterinaria Hungarica

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Vascular complications are major causes of graft failure in liver transplantation. The use of different vascular grafts is common but the results are controversial. The aim of this study was to create an 'ideal' arterial interponate for vascular replacements in the clinical field. An autologous, tubular graft prepared from the posterior rectus fascia sheath was used for iliac artery replacement in dogs for 1, 3, 6 and 12 months. Forty-one grafts were implanted and immunosuppression was used in separate groups. The patency rate was followed by Doppler ultrasound. Thirty-seven grafts remained patent, 2 cases with thrombosis and 2 cases with stenosis occurred. There was no evidence of necrosis or aneurysmatic formation. The histological analysis included conventional light microscopic and immunohistochemical examinations for CD34 and factor VIII. The explanted grafts showed signs of arterialisation, appearance of elastin fibres, and smooth muscle cells after 6 months. Electron microscopy showed intact mitochondrial structures without signs of hypoxia. In conclusion, the autologous graft presents acceptable long-term patency rate. It is easy to handle and the concept of beneficial presence of the anti-clot mesothelium until endothelialisation seems to work. The first clinical use was already reported by our group with more than 2 years survival.

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Derivation of Human Microvascular Endothelial Cells for Prosthetic Vascular Graft Seeding

Cited by 18 publications

References 9 publications

Concise Review: Therapeutic Potential of Adipose Tissue-Derived Angiogenic Cells

Concise Review: Therapeutic Potential of Adipose Tissue-Derived Angiogenic Cells

Morphological evaluation of experimental autologous rectus fascia sheath vascular grafts used for arterial replacement in a dog model

Experimental results and clinical impact of using autologous rectus fascia sheath for vascular replacement

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