Deregulation of miR-146a expression in a mouse model of pancreatic cancer affecting EGFR signaling

Ali, Shadan; Ahmad, Aamir; Aboukameel, Amro; Ahmed, Alia; Bao, Bin; Banerjee, Sanjeev; Philip, Philip A.; Sarkar, Fazlul H.

doi:10.1016/j.canlet.2014.05.013

Cited by 42 publications

(34 citation statements)

References 36 publications

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“…Overexpression of miR‐146a was confirmed to suppress the migration and invasion of gastric cancer cells . Deregulation expression of miR‐146a affected EGFR signaling in pancreatic cancer model . In this study, it was found that miR‐146a was significantly low expression in prostate cancer tissues, suggesting possible biological significance in tumorigensis of prostate cancer.…”

Section: Discussionmentioning

confidence: 56%

LncRNA PVT1 regulates prostate cancer cell growth by inducing the methylation of miR‐146a

et al. 2016

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Prostate cancer is the third most common causes of death from cancer in men. Our previous study demonstrated that lncRNA PVT1 was overexpressed and played an oncogenic role in the progression of prostate cancer. However, the molecular mechanism of modulating the prostate cancer tumorigenesis was still unknown. In this study, we aim to investigate the interaction between PVT1 and miR‐146a in prostate cancer and reveal the potential mechanism in prostate cancer carcinogenesis. The expression level of miR‐146a was assessed by quantitative RT‐PCR. The correlation analysis and methylation status analysis was made to confirm the interaction between PVT1 and miR‐146a. Biological function analysis was performed through gain‐of‐function and loss‐of‐function strategies. Our results showed that miR‐146a was downregulated and negatively correlated with PVT1 level in prostate cancer. PVT1 mediated miR‐146a expression by inducing the methylation of CpG Island in its promoter. miR‐146a overexpression eliminated the effects of PVT1 knockdown on prostate cancer cells. PVT1 regulated prostate cancer cell viability and apoptosis depending on miR‐146a. Our study suggested a regulatory relationship between lncRNA PVT1 and miR‐146a during the process of the prostate cancer tumorigenesis. PVT1 regulated prostate cancer cell viability and apoptosis depending on miR‐146a. It would contribute to the diagnosis, treatment and prognosis of prostate cancer.

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Section: Discussionmentioning

confidence: 56%

LncRNA PVT1 regulates prostate cancer cell growth by inducing the methylation of miR‐146a

et al. 2016

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“…Receptor activation leads to pro-growth and proliferation signaling Peta et al (2017), Kumaraswamy et al (2015), Ali et al (2014), Huang, He, Rong, Dang, and Chen (2014), Chen et al (2013), Xu et al (2012), Kogo et al (2011), Li et al (2010a, 2010b, Hurst et al (2009) NOTCH1/2 Endometrial, Esophageal, Glioblastoma…”

Section: Glioblastomamentioning

confidence: 99%

miR‐146a‐5p: Expression, regulation, and functions in cancer

2019

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Cancer as we know it is actually an umbrella term for over 100 very unique malignancies in various tissues throughout the human body. Each type, and even subtype of cancer, has different genetic, epigenetic, and other cellular events responsible for malignant development and metastasis. Recent work has indicated that microRNAs (miRNAs) play a major role in these processes, sometimes by promoting cancer growth and other times by suppressing tumorigenesis. miRNAs are small, noncoding RNAs that negatively regulate expression of specific target genes. This review goes into an in‐depth look at the most recent finding regarding the significance of one particular miRNA, miR‐146a‐5p, and its involvement in cancer. Target gene validation and pathway analysis have provided mechanistic insight into this miRNA's purpose in assorted tissues. Additionally, this review outlines novel findings that suggest miR‐146a‐5p may be useful as a noninvasive biomarker and as a targeted therapeutic in several cancers. This article is categorized under: RNA in Disease and Development > RNA in Disease Regulatory RNAs/RNAi/Riboswitches > Regulatory RNAs

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“…In gastric cancer, growing evidence has revealed the anti-tumor function of miR-146a-5p; it functions as a protective factor to repress neoplasm metastasis and tissue infiltration and to improve overall survival [47,48]. Recent studies have revealed that miR-146a-5p expression is lower in pancreatic cancer tissues than in non-tumor tissues based on analyses of miR-146a-5p levels in human tissue samples, cell lines, and mouse models by real-time PCR [49,50]. Our previous studies have confirmed that miR-146a-5p could clearly inhibit the deterioration of hepatocellular carcinoma [51,52].…”

Section: Discussionmentioning

confidence: 99%

Protective potential of miR-146a-5p and its underlying molecular mechanism in diverse cancers: a comprehensive meta-analysis and bioinformatics analysis

Dang

et al. 2019

Cancer Cell Int

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Background/aims: Studies have shown that miR-146a-5p was differentially expressed in diverse cancers, but the associations between miR-146a-5p expression and prognosis across multiple types of cancer as well its potential targets and downstream pathways have not been comprehensively analyzed. In this study, we performed the first meta-analysis of the prognostic value of miR-146a-5p expression in diverse malignancies and explored prospective targets of miR-146a-5p and related signaling pathways. Methods: A thorough search for articles related to miR-146a-5p was performed, and RNA-seq data from The Cancer Genome Atlas (TCGA) and microarray data from gene expression omnibus profiles were used to collect information about the prognostic value of miR-146a-5p. A comprehensive meta-analysis was conducted. Twelve platforms in miRWalk 2.0 were applied to predict targets of miR-146a-5p. TCGA RNA-seq data were used to validate the inverse relationships between miR-146a-5p and its likely targets. Subsequently, gene ontology and pathway analyses were conducted using Funrich version 3.1.3. Potential protein-protein interaction (PPI) networks were constructed. Potential target genes of miR-146a-5p in lung cancer were validated by RT-qPCR. Results: We included 10 articles in the meta-analysis. In a pooled analysis, the high miR-146a-5p expression group showed a better overall survival in solid cancers, particularly in reproductive system cancers and digestive system cancers. A total of 120 predicted target genes were included in a bioinformatics analysis. Five pathways involving phospholipase C (PLC) and aquaporins (AQPs) were the most significantly enriched Kyoto Encyclopedia of Genes and Genomes pathways. Moreover, the PPI network displayed the related signaling pathways and interactions among proteins. AQP1 and FYN were validated by RT-qPCR to be potential targets of miR-146a-5p in lung cancer. Conclusion: There is a close link between high miR-146a-5p expression and better overall survival in 21 types of solid cancer, especially in reproductive system and digestive system cancers. Furthermore, miR-146a-5p could inhibit diverse malignancies by modulating pathways linked to PLC or AQPs. In summary, miR-146a-5p is a potential prognostic biomarker and therapeutic target for various cancers.

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Deregulation of miR-146a expression in a mouse model of pancreatic cancer affecting EGFR signaling

Cited by 42 publications

References 36 publications

LncRNA PVT1 regulates prostate cancer cell growth by inducing the methylation of miR‐146a

LncRNA PVT1 regulates prostate cancer cell growth by inducing the methylation of miR‐146a

miR‐146a‐5p: Expression, regulation, and functions in cancer

Protective potential of miR-146a-5p and its underlying molecular mechanism in diverse cancers: a comprehensive meta-analysis and bioinformatics analysis

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