Depletion of circ-BIRC6, a circular RNA, suppresses non-small cell lung cancer progression by targeting miR-4491

Zhu, Jun; Gao, Baoan; Gong, Yuan; Guan, Li

doi:10.5582/bst.2020.03310

Cited by 12 publications

(10 citation statements)

References 24 publications

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“…[ 22 ] Of note, Yang et al [ 10 ] declared that circBIRC6 was overexpressed in NSCLC and exerted onco [2 g 3 e ] nic properties by acting as miR-145 sponge. Jin etal [ 23 ] revealed that circBIRC6silencing inhibited NSCLC cell proliferation and metastasis and repressed apoptosis via targeting miR-4491. In the present research, the function of circBIRC6 in NSCLC was further determined.…”

Section: Discussionmentioning

confidence: 99%

circBIRC6 contributes to the development of non-small cell lung cancer via regulating microRNA-217/amyloid beta precursor protein binding protein 2 axis

Teng

Cheng

et al. 2022

Chinese Medical Journal

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Background: Circular RNAs (circRNAs) are considered to be important regulators in cancer biology. In this study, we focused on the effect of circRNA baculoviral inhibitor of apoptosis protein (IAP) repeat containing 6 (circBIRC6) on non-small cell lung cancer (NSCLC) progression. Methods: The NSCLC and adjacent non-tumor tissues were collected at Shanghai Ninth People's Hospital. Quantitative real-time polymerase chain reaction was conducted for assessing the levels of circBIRC6, amyloid beta precursor protein binding protein 2 (APPBP2) messenger RNA (mRNA), baculoviral IAP repeat containing 6 mRNA (BIRC6), and microRNA-217 (miR-217). Western blot assay was adopted for measuring the protein levels of APPBP2, E-cadherin, N-cadherin, and vimentin. Colony formation assay, transwell assay, and flow cytometry analysis were utilized for evaluating cell colony formation, metastasis, and apoptosis. Dualluciferase reporter assay and RNA immunoprecipitation assay were carried out to determine the interaction between miR-217 and circBIRC6 and APPBP2 in NSCLC tissues. The murine xenograft model assay was used to investigate the function of circBIRC6 in tumor formation in vivo. Differences were analyzed via Student's t test or one-way analysis of variance. Pearson's correlation coefficient analysis was used to analyze linear correlation. Results: CircBIRC6 was overexpressed in NSCLC tissues and cells. Knockdown of circBIRC6 repressed the colony formation and metastasis and facilitated apoptosis of NSCLC cells in vitro and restrained tumorigenesis in vivo. Mechanically, circBIRC6 functioned as miR-217 sponge to promote APPBP2 expression in NSCLC cells. MiR-217 inhibition rescued circBIRC6 knockdown-mediated effects on NSCLC cell colony formation, metastasis, and apoptosis. Overexpression of miR-217 inhibited the malignant phenotypes of NSCLC cells, while the effects were abrogated by elevating APPBP2. Conclusions: CircBIRC6 aggravated NSCLC cell progression by elevating APPBP2 via sponging miR-217, which might provide a fresh perspective on NSCLC therapy. Keywords: Non-small cell lung cancer; Circular RNA baculoviral inhibitor of apoptosis protein repeat containing 6; MicroRNA-217; Amyloid beta precursor protein binding protein 2

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Section: Discussionmentioning

confidence: 99%

circBIRC6 contributes to the development of non-small cell lung cancer via regulating microRNA-217/amyloid beta precursor protein binding protein 2 axis

Teng

Cheng

et al. 2022

Chinese Medical Journal

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“…In another work on NSCLC result indicated that circ-BIRC6 functions as a critical regulator of growth and apoptosis in NSCLC cells via sponging miR-4491. Depletion of this CircRNA suppressed NSCLC growth by targeting miR-4491 [21].…”

Section: Discussionmentioning

confidence: 99%

High Percentage of Cancer Stem Cells in Metastatic Locations: Upregulation of circBIRC6 in Highly Metastatic Breast Cancer Subline

Kamalabadi-Farahani

Karimi

Atashi

2022

Preprint

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Metastasis is a devastating complication in breast cancer. Cancer relapse and metastasis are associated with cancer stem cells. CircBIRC6 is a circular RNA that proposed to be involved in stemness of stem cells. In breast cancer, metastatic tumor cells have higher stem cell properties. In the present study we evaluate the expression of circBIRC6 in these cells. After development of syngenic animal model of TNBC, primary breast cancer cells named 4T1T were isolated from tumor mass. Highly metastatic tumor cells named 4T1B and 4T1L were isolated and expanded from brain metastasis lesions and lung of cancerous mice respectively. Sphere formation ability in metastatic and primary tumor cells was evaluated separately. Quantitative real-time polymerase chain reaction was performed to analyze the expression of circBIRC6 in primary and metastatic tumor cells. Our data revealed that, sphere formation ability among metastatic tumor cells was significantly higher. Surprisingly expression of circBIRC6 was significantly upregulated in these metastatic tumor cells. In comparison with 4T1T, circBIRC6 was upregulated 5.7 and 3.5 time in 4T1B and 4T1L respectively. These findings provided important insights regarding the molecular properties of metastatic tumor cells and can be used to design targeted therapeutic strategies to combat these cells.

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“…It can be classified into non-small-cell lung cancer (NSCLC) and small-cell lung cancer, and NSCLC accounts for the overwhelming majority of lung cancer cases [253][254][255]. Wnt pathwayassociated circRNAs of NSCLC are shown in Table 1 [69,[175][176][177][178][179][180][181][212][213][214][215][216] (Fig. 4).…”

Section: The Respiratory System Tumor Lung Cancermentioning

confidence: 99%

The functional roles of the circRNA/Wnt axis in cancer

Xue

Zheng

et al. 2022

Mol Cancer

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CircRNAs, covalently closed noncoding RNAs, are widely expressed in a wide range of species ranging from viruses to plants to mammals. CircRNAs were enriched in the Wnt pathway. Aberrant Wnt pathway activation is involved in the development of various types of cancers. Accumulating evidence indicates that the circRNA/Wnt axis modulates the expression of cancer-associated genes and then regulates cancer progression. Wnt pathway-related circRNA expression is obviously associated with many clinical characteristics. CircRNAs could regulate cell biological functions by interacting with the Wnt pathway. Moreover, Wnt pathway-related circRNAs are promising potential biomarkers for cancer diagnosis, prognosis evaluation, and treatment. In our review, we summarized the recent research progress on the role and clinical application of Wnt pathway-related circRNAs in tumorigenesis and progression.

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Depletion of circ-BIRC6, a circular RNA, suppresses non-small cell lung cancer progression by targeting miR-4491

Cited by 12 publications

References 24 publications

circBIRC6 contributes to the development of non-small cell lung cancer via regulating microRNA-217/amyloid beta precursor protein binding protein 2 axis

circBIRC6 contributes to the development of non-small cell lung cancer via regulating microRNA-217/amyloid beta precursor protein binding protein 2 axis

High Percentage of Cancer Stem Cells in Metastatic Locations: Upregulation of circBIRC6 in Highly Metastatic Breast Cancer Subline

The functional roles of the circRNA/Wnt axis in cancer

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