Deoxynivalenol concentrations in feed ingredients and swine diets measured by enzyme-linked immunosorbent assay and high-performance liquid chromatography

Ahn, Jong Young; Cheong, Dahyun; Jeong, Jin Young; Kim, Beob Gyun

doi:10.37496/rbz5220220155

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…For analyzing chromium concentrations in the diet and ileal digesta, an ultraviolet-visible spectrophotometry (Optizen 2120UV, Mecasys Inc., Daejeon, Republic of Korea) was used. The concentrations of DON in the ingredient samples and experimental diets were determined using a high-performance liquid chromatography with an immunoaffinity chromatography column (ZORBAX Eclipse XDB-C18 column, 4.6 × 250 mm, 5 µM, Agilent, Santa Clara, CA, USA; [22]).…”

Section: Chemical Analysesmentioning

confidence: 99%

Effects of a Bentonite Clay Product and a Preservative Blend on Ileal and Fecal Nutrient Digestibility in Pigs Fed Wheat Naturally Contaminated with Deoxynivalenol

Shin,

Yoo,

Song

et al. 2023

Animals

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The objectives were to determine the effects of dietary deoxynivalenol (DON) on apparent ileal digestibility (AID) of nutrients and to evaluate the efficacy of a bentonite (BEN) and a preservative blend (PB) product for alleviating DON effects on the nutrient digestibility of pigs. Twelve crossbred barrows with an initial body weight of 69.4 kg (standard deviation = 3.5) equipped with a T-cannula in the distal ileum were allotted a triplicated 4 × 2 incomplete Latin square design with four dietary treatments and two periods. Dietary treatments were (1) an uncontaminated diet, (2) a contaminated diet (CD) mainly based on contaminated wheat with 1.6 mg/kg DON, (3) CD + 0.25% PB consisting of preservation components as major sources, antioxidants, microorganisms, and amino acids (AA), and (4) CD + 0.25% BEN. The AID and ATTD of dry matter, organic matter, crude protein, most minerals, and most AA were not affected by DON contamination. Dietary DON decreased the AID and ATTD of sodium (p < 0.05) but were restored by supplementing the PB product (p < 0.05). The AID of zinc was increased (p < 0.05) by dietary DON, but supplementing BEN decreased zinc digestibility (p < 0.05). The AID of Arg, Ile, Thr, and Asp was decreased (p < 0.05) by BEN addition. In conclusion, dietary DON affected the digestibility of some minerals but not AA in pigs. Supplemental BEN can negatively affect the nutrient digestibility of some minerals and AA in pigs. The addition of a PB product in pig diets can restore digestibility of sodium but not of other nutrients. Based on these observations, feed additives for alleviating DON effects on nutrient digestibility of pigs can be carefully selected by swine diet formulators.

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Section: Chemical Analysesmentioning

confidence: 99%