Dental Tissue Engineering

Monteiro, Nelson; Yelick, Pamela C.

doi:10.1016/b978-0-12-809880-6.00051-5

Cited by 5 publications

(4 citation statements)

References 96 publications

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“…[160] To promote dental differentiation, growth factors delivered by specific vehicles may be used to control the specific signaling pathway and gene expression of stem cells within a site of injury and stimulate them to differentiate into bone or dentin forming cells. [161] BMPs are a group of growth factors that are of particular importance in bone tissue engineering. [162,163] The effects of BMP-4 [164] and BMP-9 [165] on stem cell differentiation has been well reported in dental tissue engineering.…”

Section: Delivery Of Growth Factorsmentioning

confidence: 99%

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Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

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The oral cavity and oropharynx are complex environments that are susceptible to physical, chemical, and microbiological insults. They are also common sites for pathological and cancerous changes. The effectiveness of conventional locally-administered medications against diseases affecting these oral milieus may be compromised by constant salivary flow. For systemically-administered medications, drug resistance and adverse side-effects are issues that need to be resolved. New strategies for drug delivery have been investigated over the last decade to overcome these obstacles. Synthesis of nanoparticle-containing agents that promote healing represents a quantum leap in ensuring safe, efficient drug delivery to the affected tissues. Micro/nanoencapsulants with unique structures and properties function as more favorable drug-release platforms than conventional treatment approaches. The present review provides an overview of newly-developed nanocarriers and discusses their potential applications and limitations in various fields of dentistry and oral medicine.

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Section: Delivery Of Growth Factorsmentioning

confidence: 99%

Section: Gene and Protein Deliverymentioning

confidence: 99%

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

et al. 2021

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“…Mammalian dental mesenchymal tissues possess a limited capacity to regenerate dentin, cementum, and pulp cells (Gronthos et al 2000); thus, they play an important role in tooth homeostasis and repair. Many attempts to isolate cells from human dental pulp for regeneration applications have been reported (Miura et al 2003; Monteiro and Yelick 2019).…”

Section: The Mouse Incisor Mesenchymementioning

confidence: 99%

From Bite to Byte: Dental Structures Resolved at a Single-Cell Resolution

et al. 2021

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The systematic classification of the cells that compose a tissue or an organ is key to understanding how these cells cooperate and interact as a functional unit. Our capacity to detect features that define cell identity has evolved from morphological and chemical analyses, through the use of predefined genetic markers, to unbiased transcriptomic and epigenetic profiling. The innovative technology of single-cell RNA sequencing (scRNA-seq) enables transcriptional profiling of thousands of individual cells. Since its development, scRNA-seq has been extensively applied to numerous organs and tissues in a wide range of animal models and human samples, thereby providing a plethora of fundamental biological insights into their development, homeostasis, and pathology. In this review, we present the findings of 3 recent studies that employed scRNA-seq to unravel the complexity of cellular composition in mammalian teeth. These findings offer an unprecedented catalogue of cell types in the mouse incisor, which is a convenient model system for studying continuous tooth growth. These studies identified novel cell types in the tooth epithelium and mesenchyme, as well as new markers for known cell types. Computational analyses of the data also uncovered the lineage and dynamics of cell states during ameloblast and odontoblast differentiation during both normal homeostasis and injury repair. The transcriptional differences between the mouse incisor and mouse and human molars uncover species-specific as well as shared features in tooth cell composition. Here, we highlight these findings and discuss important similarities and differences between these studies. We also discuss potential future applications of scRNA-seq in dental research and dentistry. Together, these studies demonstrate how the rapidly evolving technology of scRNA-seq can advance the study of tooth development and function and provide putative targets for regenerative approaches.

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“…Tissue engineering could offer such promising solution to regenerate damaged dental tissues including enamel [1]. Scaffolds are one of the main elements for tissue engineering that need to possess desirable physical (swelling, degradation, mechanical) and biological and functional characteristics to support the new tissue formation [67].…”

Section: Discussionmentioning

confidence: 99%

Bioprinting of alginate-carboxymethyl chitosan scaffolds for enamel tissue engineering in vitro

et al. 2022

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Three dimensional (3D) bioprinting has been emerging enabling the incorporation of living cells into the biomaterials (such a mixture is referred as a bioink) to create scaffolds. However, the bioinks available for scaffold bioprinting are limited, particularly for dental tissue engineering, due to the complicated, yet compromised, printability, mechanical and biological properties simultaneously imposed on the bioinks. This paper shows the development of a novel bioink from carboxymethyl chitosan and alginate for bioprinting scaffolds for enamel tissue regeneration. Carboxymethyl chitosan is used because to its antibacterial ability and superior cell interaction properties, while alginate was added to enhance printability and mechanical properties as well as to regulate the degradation rate. The bioinks with three mixture ratios of alginate and carboxymethyl chitosan (2-4, 3-3 and 4-2) are prepared, and then printed into the calcium chloride crosslinker solution (100 mM) to form a 3D structure of scaffolds. The printed scaffolds are characterized in terms of structural, swelling, degradation, and mechanical properties, followed by their in vitro characterization for enamel tissue regeneration. The results show that the bioinks with higher concentrations of alginate are more viscous and need higher pressure for printing; while the printed scaffolds are highly porous and show a high degree of printability and structural integrity. The hydrogels with higher carboxymethyl chitosan ratios have higher swelling ratios, faster degradation rates, and lower compressive modulus. Dental epithelial cell line, HAT-7, could maintain high viability in the printed constructs after 1, 7 and 14 days of culture. HAT-7 cells are also able to maintain their morphology and secrete alkaline phosphatase after 14 days of culture in the 3D printed scaffolds, suggesting the capacity of these cells for mineral deposition and enamel-like tissue formation. Taken together, alginate-carboxymethyl chitosan may be suitable to print scaffolds with dental epithelial cells for enamel tissue regeneration.

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Dental Tissue Engineering

Cited by 5 publications

References 96 publications

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

Drug Delivery (Nano)Platforms for Oral and Dental Applications: Tissue Regeneration, Infection Control, and Cancer Management

From Bite to Byte: Dental Structures Resolved at a Single-Cell Resolution

Bioprinting of alginate-carboxymethyl chitosan scaffolds for enamel tissue engineering in vitro

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