Dental stem cell and dental tissue regeneration

Zhai, Qiming; Dong, Zhiwei; Wang, Wei; Li, Bei; Jin, Yan

doi:10.1007/s11684-018-0628-x

Cited by 138 publications

(121 citation statements)

References 54 publications

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“…28,29 Stem cells from apical papilla showed significantly high mineralization and proliferation. 30,31 Even in an immature tooth with pulp necrosis and AP, human apical papilla retained SCAP vitality. 32 Therefore, we selected hSCAPs to probe into the mechanism of root repair.…”

Section: Discussionmentioning

confidence: 99%

Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation

Cui

Xie

et al. 2020

Int J Oral Sci

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Once pulp necrosis or apical periodontitis occurs on immature teeth, the weak root and open root apex are challenging to clinicians. Berberine (BBR) is a potential medicine for bone disorders, therefore, we proposed to apply BBR in root canals to enhance root repair in immature teeth. An in vivo model of immature teeth with apical periodontitis was established in rats, and root canals were filled with BBR, calcium hydroxide or sterilized saline for 3 weeks. The shape of the roots was analyzed by micro-computed tomography and histological staining. In vitro, BBR was introduced into stem cells from apical papilla (SCAPs). Osteogenic differentiation of stem cells from apical papilla was investigated by alkaline phosphatase activity, mineralization ability, and gene expression of osteogenic makers. The signaling pathway, which regulated the osteogenesis of SCAPs was evaluated by quantitative real time PCR, Western blot analysis, and immunofluorescence. In rats treated with BBR, more tissue was formed, with longer roots, thicker root walls, and smaller apex diameters. In addition, we found that BBR promoted SCAPs osteogenesis in a time-dependent and concentration-dependent manner. BBR induced the expression of β-catenin and enhanced β-catenin entering into the nucleus, to up-regulate more runt-related nuclear factor 2 downstream. BBR enhanced root repair in immature teeth with apical periodontitis by activating the canonical Wnt/β-catenin pathway in SCAPs.

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Section: Discussionmentioning

confidence: 99%

Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation

Cui

Xie

et al. 2020

Int J Oral Sci

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“…Stem/progenitor cells derived from oral cavity express several mesenchymal markers including CD29, CD73, CD90, and CD105 as well as embryonic markers such as Sox2, Nanog and Oct4 and can differentiate into multiple cell lineages [11,13,[30][31][32][33]. Importantly, some dental stem cells exhibit more embryonic-like features than bone marrow stem cells and umbilical cord [31][32][33][34]. Therefore, mesenchymal stem cells derived from the oral cavity are believed to be very important and valuable resource for eventual development of cells for clinical/therapeutic applications not only in dentistry but also in medicine.…”

Section: Dental Stem Cellsmentioning

confidence: 99%

Stem cells and tooth regeneration: prospects for personalized dentistry

et al. 2019

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Over the last several decades, a wealth of information has become available regarding various sources of stem cells and their potential use for regenerative purposes. Given the intense debate regarding embryonic stem cells, much of the focus has centered around application of adult stem cells for regenerative engineering along with other relevant aspects including use of growth factors and scaffolding materials. The more recent discovery of tooth-derived stem cells has sparked much interest in their application to regenerative dentistry to treat and alleviate the most prevalent oral diseases-i.e., dental caries and periodontal diseases. Also exciting is the advent of induced pluripotent stem cells, which provides the means of using patient-derived somatic cells for their creation, and their eventual application for generation of the dental complex. Thus, evolving developments in the field of regenerative dentistry indicate the prospect of constructing Bcustom-made^tooth and supporting structures thereby fostering the realization of Bpersonalized dentistry.^On the other hand, others have explored the possibility of augmenting endogenous regenerative capacity through utilization of small molecules to regulate molecular signaling mechanisms that mediate regeneration of tooth structure. This review is focused on these aspects of regenerative dentistry in view of their relevance to personalized dentistry.

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“…Furthermore, dental stem cells are convenient to obtain, possess low immunogenicity and free of any ethical controversy, which might be good resource for tissue regeneration. 67,68 Seeding dental stem cells on appropriate biomaterials may promote their capability for bone, cartilage or dental tissue regeneration. Hu et al 69 have transplanted human DPSCs (hDPSCs) into a swine model for periodontal regeneration, and the results showed that translating hDPSCs into this large animal model could significantly improve periodontal bone regeneration and soft tissue healing.…”

Section: Dna Nanostructures Have Applications In a Wide Array Of Fielmentioning

confidence: 99%

Review of craniofacial regeneration in China

Zhang

Xie

et al. 2019

J of Oral Rehabilitation

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Craniofacial defect is frequently caused by inflammation, trauma, and tumour etc The damaged tissues cannot be fully reconstituting the integrated matrix and regenerate native surface due to the complexity of craniofacial tissues, although with good intrinsic healing capacity. Till now, many approaches, such as manufacturing prosthesis, have obtained limited results. Tissue engineering has been recognised as one of the most effective means to replace or repair damaged tissues, which aims at forming a new viable tissue for a medical purpose. Such approaches need scaffolds that support the attachment, growth and proliferation of cells. With the development of modern medicine, the research of biomaterials has undergone a process from bio-inert materials to bioactive or degradable materials. The first generation of biomaterials was bio-inert materials with high strength, which included the metallic materials, ceramic materials, and polymeric materials. Whereas second-generation biomaterials were designed to be either resorbable or bioactive, the new biomaterials converged the separate concepts of bioactive materials and resorbable materials. Ideally, the scaffold must be of good biocompatibility and suitable physical and mechanical properties, furthermore, the scaffold must possess three-dimensional and properly porous network to allow cell adhesion, and controlled rate of degradation to match new tissue growth in vitro and in vivo. 1,2 Besides, the mechanism of tissue repair is complex and involves several important bioactive factors, 3,4 which function as inductive signals to provide cells with sufficient stimulus to migrate into tissues or biomaterials. Utilisation of growth factors, peptides or small molecules that associated with a natural matrix or biomaterial carrier has been extensively studied. Tissue engineering also utilises living cells as engineering materials, and cells became available as engineering materials when Bodnar et al 5 discovered how to extend telomeres in 1998, producingimmortalised cell lines. Stem cells are self-renewing and multipotent, with unique capacity to regenerate

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Dental stem cell and dental tissue regeneration

Cited by 138 publications

References 54 publications

Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation

Berberine mediates root remodeling in an immature tooth with apical periodontitis by regulating stem cells from apical papilla differentiation

Stem cells and tooth regeneration: prospects for personalized dentistry

Review of craniofacial regeneration in China

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