Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network

Roozafzoon, Reza; Lashay, Alireza; Vasei, Mohammad; Ai, Jafar; Khoshzaban, Ahad; Keshel, Saeed Heidari; Barabadi, Zahra; Bahrami, Hoda

doi:10.1016/j.bbrc.2014.12.069

Cited by 45 publications

(53 citation statements)

References 32 publications

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“…Arvand J Health Med Sci 2016;1(1): [17][18][19][20][21][22][23][24][25][26] from hepatic or non-hepatic stem cells, are therefore considered as potential alternatives (1). In particular, mesenchymal stem cells (MSC) from cord blood (CB), adipose tissue, or bone marrow are stated as a potential stem cell source for hepatocyte or hepatocyte-like cell generation in both in vivo and in vitro approaches (2)(3)(4)(5).…”

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

“…Fibrin is a natural suitable polymer widely applied in tissue engineering applications (19)(20)(21)(22). Fibrinogen is converted into fibrin through the reaction of thrombin in the presence of high amounts of calcium ions, during fibrin network formation (22).…”

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

“…Fibrinogen is converted into fibrin through the reaction of thrombin in the presence of high amounts of calcium ions, during fibrin network formation (22). These reactions make 3-dimensional organized clot resembling classic wound healing which accrue in the body (22,23). It is to remember that beside of its elasticity and biocompatibility, it provides suitable mechanical support for cell migration and cell entrapment (22).…”

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

“…These reactions make 3-dimensional organized clot resembling classic wound healing which accrue in the body (22,23). It is to remember that beside of its elasticity and biocompatibility, it provides suitable mechanical support for cell migration and cell entrapment (22).…”

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

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Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Tavirani

Keshel

Takcalou

2016

Arvand Journal of Health and Medical Sciences

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Reports describing the human cord blood derived mesenchymal stem cells (hUSSCs) hepatocytic differentiation potential are rare and there is no report describing fibrin base 3D culture of hepatocyte-like cells differentiated from cord blood unrestricted somatic stem cells. Hepatocyte-like differentiated stem cells has the potential to provide compensation for acute liver failure patients. Therefore, we characterized cord blood Unrestricted Somatic Stem Cells (USSC) and their hepatocyte differentiation capabilities for functional liver support in a three dimensional arrangement. Moreover, three-dimensional (3D) network and its distribution to grow and differentiate stem cells in so far as possible mimic to native tissue holds huge potential in liver tissue engineering. In this study, we isolate USSCs from human cord blood and validate them with flow-cytometry. Briefly, we differentiated cells using DMEM containing FGF4, Activin A, HGF and 2% FBS into hepatocyte-like cells (HLCs) in two conditions; 3D state in biocompatible fibrin Hydrogel and or conventional culture (2D) in polystyrene plates for 21 days. Immuncytochemical and gene expression analysis revealed the expression of KRT18, KRT19, albumin, Sox17, FoxA2 and Prox1 increased in 3D fibrin culture compared to 2D conventional culture. In combination, these data reveal that using 3D networks can resemble near natural tissue properties for effective generating HLCs which used to cell replacement in the future. As well as, USSC represent a stem cell source with a substantial hepatic differentiation capability which hold the possible for clinical applications.

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Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

Section: Original Article 18 Unrestricted Somatic Stem Cells Differenmentioning

confidence: 99%

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Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Tavirani

Keshel

Takcalou

2016

Arvand Journal of Health and Medical Sciences

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Concise Review: Dental Pulp Stem Cells: A Novel Cell Therapy for Retinal and Central Nervous System Repair

et al. 2016

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Dental pulp stem cells (DPSC) are neural crest-derived ecto-mesenchymal stem cells that can relatively easily and non-invasively be isolated from the dental pulp of extracted postnatal and adult teeth. Accumulating evidence suggests that DPSC have great promise as a cellular therapy for central nervous system (CNS) and retinal injury and disease. The mode of action by which DPSC confer therapeutic benefit may comprise multiple pathways, in particular, paracrinemediated processes which involve a wide array of secreted trophic factors and is increasingly regarded as the principal predominant mechanism. In this concise review, we present the current evidence for the use of DPSC to repair CNS damage, including recent findings on retinal ganglion cell neuroprotection and regeneration in optic nerve injury and glaucoma. STEM CELLS 2017;35:61-67 SIGNIFICANCE STATEMENTThis concise review discusses recent developments regarding the novel application of stem cells isolated from the dental pulp for the repair of central nervous system and retinal neurons.

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A concise review of the orofacial mesenchymal stromal cells as a novel therapy for neurological diseases and injuries

Dong

Zhao

2022

J Tissue Eng Regen Med

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Orofacial mesenchymal stromal cells (OFMSCs) are mesenchymal stromal cells isolated from the oral and facial regions, which possess typical mesenchymal stromal cell features such as self-renewing, multilineage differentiation, and immunoregulatory properties. Recently, increasing studies have been carried out on the neurotrophic and neuroregenerative properties of OFMSCs as well as their potential to treat neurological diseases. In this review, we summarize the current evidence and discuss the prospects regarding the therapeutic potential of OFMSCs as a new approach to treat different neurological diseases and injuries.

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Dental pulp stem cells differentiation into retinal ganglion-like cells in a three dimensional network

Cited by 45 publications

References 32 publications

Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Unrestricted somatic stem cells differentiation into hepatic-like cells in a three dimensional arrangement

Concise Review: Dental Pulp Stem Cells: A Novel Cell Therapy for Retinal and Central Nervous System Repair

A concise review of the orofacial mesenchymal stromal cells as a novel therapy for neurological diseases and injuries

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