Densidade das células do endotélio corneano em olhos de cães à microscopia especular

Abstract

The aim of this study was to examine the endothelial surface and to perform a morphometric analysis of the corneal endothelial cells in normal eyes of dogs using specular microscopy. Morphometric analysis with regard mean cell area and cell density was performed. Both eyes of ten mixed-breed, males and females, with 6 years of age, weighing about 15 kg euthanatized for reasons unrelated to this study were evaluated. Eyes were examined to determine that they did not have visible ocular disease and transported to the laboratory in moist chamber. Using a contact specular microscope the corneal endothelium was examined. Three images of the central corneal endothelium of each eye were obtained. The mean cell area and the cell density of the corneal endothelial cells were obtained using software for corneal endothelium analysis and density measurement. The mean cell area was 395 ± 36 µm 2 and the endothelial cell density was 2555 ± 240 cells/mm 2 . The present work demonstrates that the normal corneal endothelium of dog is similar to those described in human.

V-Prechop nucleodissection technique feasibility in the phacoemulsification in dogs with cataracts. Clinical aspects and specular microscopy

“…It begins immediately after trauma or exposure to allergens or toxins so was expected immediately after phacoemulsification (Munger, 2009). There were no postoperative complications identified such as fibrovascular membranes, growth of lenticular fibers, lenticular epithelial membranes, or endophthalmitis (Munger, 2009) It can be inferred that the presence of immature or mature cataracts does not influence the density values or cell area of the corneal endothelial cells, as the values obtained preoperatively did not differ between the groups and were not as described by Chiurciu et al (2010) and Pigatto et al (2006).…”

“…This technique is called "V-prechop". The evaluation of postoperative clinical parameters may help to predict whether or not the technique is proposed, as well as specular microscopy (Munger, 2009;Pigatto et al, 2006;Padua et al, 2017).…”

Effects of atracurium besylate on corneal endothelium of chickens: in vitro study

“…Among used techniques to analyze the corneal endothelium are including mainly SEM, specular microscopy, and optical microscopy (Pigatto et al 2006, Nagatsuyu et al 2014, Kobashigawa et al 2015, Coyo et al 2016, Faganello et al 2016.…”

“…SEM has been applied mainly for the analysis of endothelial cell ultrastructure of different species of animals and to evaluate the effects of medications, chemicals or surgical procedures on the endothelium (Pigatto et al 2009, Tamayo-Arango et al 2009, Terzariol et al 2016. Specular microscopy is the technique most used for clinical evaluations in humans and animals (Pigatto et al 2006, Franzen et al 2010, Nagatsuyu et al 2014, Bercht et al 2015, Kobashigawa et al 2015, Coyo et al 2016. Among the limitations of specular microscopy technique is the difficulty of obtaining images in corneas that are not transparent (Saad et al 2008).…”

“…Among the main methods used for endothelial analysis are specular microscopy, confocal microscopy and scanning electron microscopy (Pigatto et al 2006, Nagatsuyu et al 2014, Kobashigawa et al 2015, Coyo et al 2016, Terzariol et al 2016. In addition, it is possible to analyze and photograph the endothelium of the cornea under an optical microscope after staining with alizarin red dye (Saad et al 2008, Ruggeri et al 2009, Park et al 2012.…”