Dendritic Cells in Cord Blood Transplantation: A Review

Pereira, Marta Isabel; Paiva, Artur

doi:10.4061/2011/539896

Cited by 6 publications

(5 citation statements)

References 74 publications

(88 reference statements)

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“…The previously discussed DC subsets can be found in UCB; however, they differ in their frequency and function to adult peripheral blood [ 33 , 34 , 56 ]. In contrast to their adult counterparts, DC phenotypes are skewed toward immaturity, marked by reduced antigen uptake as a consequence of lower expression of the immunoglobulin (Ig) G receptors CD32 and CD64 [ 57 ] and lower expression of co-stimulatory molecules, including MHC class II, ICAM-1, CD80, and CD86 [ 58 ]. Furthermore, UCB-derived DC subsets secrete less TNFα, IL-12p70, and IFNγ following TLR stimulation [ 33 ].…”

Section: Apc Biology In Adult and Umbilical Cord Blood—what Is The Difference?mentioning

confidence: 99%

Cord-Blood-Derived Professional Antigen-Presenting Cells: Functions and Applications in Current and Prospective Cell Therapies

Cunningham

Hackstein

2021

IJMS

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Human umbilical cord blood (UCB) represents a valuable source of hematopoietic stem cells, particularly for patients lacking a matching donor. UCB provides practical advantages, including a lower risk of graft-versus-host-disease and permissive human leukocyte antigen mismatching. These advantageous properties have so far been applied for stem cell, mesenchymal stromal cell, and chimeric antigen receptor T cell therapies. However, UCB-derived professional antigen-presenting cells are increasingly being utilized in the context of immune tolerance and regenerative therapy. Here, we review the cell-specific characteristics as well as recent advancements in UCB-based cell therapies focusing on dendritic cells, monocytes, B lymphocytes, innate lymphoid cells, and macrophages.

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Section: Apc Biology In Adult and Umbilical Cord Blood—what Is The Difference?mentioning

confidence: 99%

Cord-Blood-Derived Professional Antigen-Presenting Cells: Functions and Applications in Current and Prospective Cell Therapies

Cunningham

Hackstein

2021

IJMS

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“…Moreover, dendritic cells (DCs) play a major part in transplant engraftment and rejection and in graft-versus-host disease (GvHD). Preferentially tolerogenic or immunogenic DC subtypes offer targets for immunotherapy, to optimise transplant success rates and prolong disease-free and overall survival [ 4 ]. Advances in the extraction and in vitro culture of DCs have been a major driving force behind the increased interest in these cells and have facilitated the inclusion of these powerful adjuvants in therapeutic trials.…”

Section: Introductionmentioning

confidence: 99%

Experimental immunology Comparison between magnetic activated cell sorted monocytes and monocyte adherence techniques for in vitro generation of immature dendritic cells: an Egyptian trial

El-Sahrigy

Mohamed

Talkhan

et al. 2015

cejoi

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IntroductionDendritic cells (DCs) are the most efficient antigen presenting cells, which are considered a central component of the immune system for their extraordinary capacity to initiate and modulate the immune responses elicited upon recognition of infectious agents. This has made them a major focus of interest in the conception of immunotherapeutic vaccine strategies.Aim of the studyTo standardise a protocol for in vitro differentiation of human peripheral blood monocytes into immature DCs (iDCs) upon treatment with specific growth factors and to compare two monocyte isolation methods including magnetic activated cell sorted (MACS) monocytes by CD14+ immuno-magnetic beads and monocytes separated by adherence.Material and methodsImmature DCs were generated from monocytes of human peripheral blood in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin (IL)-4 after in vitro culture for seven days. Cultured cells were stained with surface markers of iDCs: FITC-anti-CD14, PE-anti-CD11c, PE-anti-CD1a, PE-Cy5-anti-HLA-DR, and PE-anti-CD83 for flow cytometry analysis.ResultsWe found that the viability of MACS-DCs was higher than DCs derived from monocytes separated by adherence (median 50 and interquartile range 45-50 vs. 25 and 10-30, respectively; p < 0.001). Flow cytometry analysis revealed that the median interquartile percentages of MACS-DCs expressing CD14– was significantly higher compared to the DCs derived from monocytes separated by adherence (median 80.2 and interquartile range 77.7-80.7 vs. 40.2 and 30.4-40.6, respectively; p < 0.001). However, MACS-DCs expressed the same levels of CD11c, CD1a, and HLA-DR as well as CD83 compared to the DCs derived from monocytes separated by adherence with p value > 0.05.ConclusionsBoth positively selected monocytes and monocytes separated by adherence procedure gave the same results as regards cell surface marker expression, although the DCs purity and viability using MACS separated monocytes were better.

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“…It is also well accepted that dendritic cells (DCs) represent a heterogeneous population of potent lineage-negative HLA-DR+ antigen-presenting cells [ 12 ]. It is possible to identify at least two subsets of circulating DCs on the blood [ 8 ]; the myeloid DC (mDC) subpopulation , strongly expressing HLA-DR and the myeloid-associated antigens CD11c and CD33 and the plasmacytoid DC (pDCs) subset , expressing high levels of both HLA-DR and CD123, without expressing myeloid associated antigens [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Sera from patients with active systemic lupus erythematosus patients enhance the toll-like receptor 4 response in monocyte subsets

Carvalheiro¹,

Gomes²,

Pinto

et al. 2015

J Inflamm

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BackgroundSystemic Lupus Erythematosus (SLE) is an auto-immune disease whose complex pathogenesis remains unraveled. Here we aim to explore the inflammatory ability of SLE patients’ sera upon peripheral blood (PB) monocyte subsets and myeloid dendritic cells (mDCs) obtained from healthy donors.MethodsIn this study we included 11 SLE patients with active disease (ASLE), 11 with inactive disease (ISLE) and 10 healthy controls (HC). PB from healthy donors was stimulated with patients’ sera, toll-like receptor (TLR) 4 ligand – lipopolysaccharide or both. The intracellular production of TNF-α was evaluated in classical, non-classical monocytes and mDCs, using flow cytometry. TNF-α mRNA expression was assessed in all these purified cells, after sera treatment.ResultsWe found that sera of SLE patients did not change spontaneous TNF-α production by monocytes or dendritic cells. However, upon stimulation of TLR4, the presence of sera from ASLE patients, but not ISLE, significantly increased the intracellular expression of TNF-α in classical and non-classical monocytes. This ability was related to titers anti-double stranded DNA antibodies in the serum. High levels of anti-TNF-α in the patients’ sera were associated with increased TNF-α expression by co-cultured mDCs. No relationship was found with the levels of a wide variety of other pro-inflammatory cytokines. A slight increase of TNF-α mRNA expression was observed in these purified cells when they were cultured only in the presence of SLE serum.ConclusionsOur data suggest that SLE sera induce an abnormal in vitro TLR4 response in classical and non-classical monocytes, reflected by a higher TNF-α intracellular expression. These effects may be operative in the pathogenesis of SLE.

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Dendritic Cells in Cord Blood Transplantation: A Review

Cited by 6 publications

References 74 publications

Cord-Blood-Derived Professional Antigen-Presenting Cells: Functions and Applications in Current and Prospective Cell Therapies

Cord-Blood-Derived Professional Antigen-Presenting Cells: Functions and Applications in Current and Prospective Cell Therapies

Experimental immunology Comparison between magnetic activated cell sorted monocytes and monocyte adherence techniques for in vitro generation of immature dendritic cells: an Egyptian trial

Sera from patients with active systemic lupus erythematosus patients enhance the toll-like receptor 4 response in monocyte subsets

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