Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers

Йонов, Максим; Ciepluch, Karol; Garaiova, Zuzana; Melikishvili, Sophie; Michlewska, Sylwia; Balcerzak, Łucja; Glińska, Sława; Miłowska, Katarzyna; Gomez-Ramirez, Rafael; Mata, F. Javier de la; Shcharbin, Dzmitry; Waczulı́ková, Iveta; Bryszewska, Maria; Hianik, Tibor

doi:10.1016/j.bbamem.2014.12.025

Cited by 21 publications

(16 citation statements)

References 31 publications

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“…Distribution of hydrophobic amino acid in MPER can modulate membrane fusion (80,84). Electrostatic interaction between viral particle and negatively charged lipid membrane may also play a role in viral entry (85). Logistic regression analysis indicated an interactive effect of hydropathy and charge of HIV-1 MPER variants on breast milk transmission outcome in our study.…”

Section: Discussionmentioning

confidence: 99%

Unique Genotypic Features of HIV-1 C Gp41 Membrane Proximal External Region Variants during Pregnancy Relate to Mother-to-child Transmission via Breastfeeding

Yin

Chang

Nakamura

et al. 2020

Preprint

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Mother-to-child transmission (MTCT) through breastfeeding remains a major source of pediatric HIV-1 infection worldwide. To characterize plasma HIV-1 subtype C populations from infected mothers during pregnancy that related to subsequent breast milk transmission, an exploratory study was designed to apply next generation sequencing and a custom bioinformatics pipeline for HIV-1 gp41 extending from heptad repeat region 2 (HR2) through the membrane proximal external region (MPER) and the membrane spanning domain (MSD). Viral populations during pregnancy from women who transmitted by breastfeeding, compared to those who did not, displayed greater biodiversity, more frequent amino acid polymorphisms, lower hydropathy index and greater positive charge. Viral characteristics were restricted to MPER, failed to extend into flanking HR2 or MSD regions, and were unrelated to predicted neutralization resistance. Findings provide novel parameters to evaluate an association between maternal MPER variants present during gestation and lactogenesis with subsequent transmission outcomes by breastfeeding.

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Section: Discussionmentioning

confidence: 99%

Unique Genotypic Features of HIV-1 C Gp41 Membrane Proximal External Region Variants during Pregnancy Relate to Mother-to-child Transmission via Breastfeeding

Yin

Chang

Nakamura

et al. 2020

Preprint

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“…Analysis of the fluorescence anisotropy changes of two fluorescent labels, DPH and TMA-DPH, that are located at different membrane depths, confirmed that all tested dendrimers interacted with both regions of membranes and changed membrane fluidity. The observed increase in the fluorescence anisotropy indicated the rising membrane stiffness [29], which in turn reflected the ability of the tested dendrimers to interact with bilayers [30,31]. Phosphorothioate dendrimers of the 5th generation with peripheral hydroxyl groups [32] and viologen-phosphorus dendrimers were also shown to enhance the stiffness of biological membranes, although the intensity of this process was lower [33].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Anticancer Properties of Copper Metallodendrimers

et al. 2019

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Newly synthesized carbosilane copper dendrimers (CCD) with chloride and nitrate surface groups seem to be good candidates to be used as gene and drug carriers in anti-cancer therapy, due to their properties such as size and surface charge. Copper attached to the nanoparticles is an important element of many biological processes and recently their anti-cancer properties have been widely examined. Zeta size and potential, transmission electron microscopy (TEM), circular dichroism (CD), analysis of haemolytic activity, and fluorescence anisotropy techniques were used to characterize copper dendrimers. Additionally, their cytotoxic properties toward normal (PBMC) and cancer (1301; HL-60) cells were examined. All tested dendrimers were more cytotoxic against cancer cells in comparison with normal cells.

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“…The higher-generation dendrimer has a correspondingly higher zeta potential [5]. Positive charges of both dendrimers indicate that they should readily interact with negatively charged cell membrane [5,23,24]. Due to interaction between dendrimers, they can form aggregates.…”

Section: Discussionmentioning

confidence: 99%

Synthesis and Characterization of FITC Labelled Ruthenium Dendrimer as a Prospective Anticancer Drug

et al. 2019

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Metallodendrimers—dendrimers with included metals—are widely investigated as biocompatible equivalents to metal nanoparticles. Applications can be expected in the fields of catalysis, as chemical sensors in molecular recognition and as anticancer drugs. Metallodendrimers can also mimic certain biomolecules, for example, haemoprotein in the case of using a dendrimer with a porphyrin core. In previous papers, we showed the promising anticancer effects of carbosilane ruthenium dendrimers. The present paper is devoted to studying biocompatibility and the cytotoxic effect on normal and cancer cells of carbosilane ruthenium dendrimers labelled with fluorescent probe fluorescein isothiocyanate (FITC). The addition of fluorescent probe allowed tracking the metallodendrimer in both normal and cancer cells. It was found that carbosilane ruthenium dendrimer labelled with FITC in concentration up to 10 µmol/L was more cytotoxic for cancer cells than for normal cells. Thus, FITC labelled carbosilane ruthenium dendrimer is a good candidate for diagnostic imaging and studying anticancer effects of metallodendrimers in cancer therapy.

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Dendrimers complexed with HIV-1 peptides interact with liposomes and lipid monolayers

Abstract: HIV-peptide/dendrimer complexes interact with model lipid membranes depending on their surface charge. Carbosilane dendrimers can be useful as non-viral carriers for delivering HIV-peptides into cells.

Cited by 21 publications

References 31 publications

Unique Genotypic Features of HIV-1 C Gp41 Membrane Proximal External Region Variants during Pregnancy Relate to Mother-to-child Transmission via Breastfeeding

Unique Genotypic Features of HIV-1 C Gp41 Membrane Proximal External Region Variants during Pregnancy Relate to Mother-to-child Transmission via Breastfeeding

In Vitro Anticancer Properties of Copper Metallodendrimers

Synthesis and Characterization of FITC Labelled Ruthenium Dendrimer as a Prospective Anticancer Drug

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