Demystifying the coordination polymers triggered fluorescence immunoassay: State of the art and future perspectives

Zhang, Qun-Zheng; Wang, Ruifei; Han, Wei; Ke, Congye; Wang, Sichang; Zhang, Xunli; Wang, Jianlong

doi:10.1016/j.ccr.2023.215188

Cited by 6 publications

(2 citation statements)

References 138 publications

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“…Theoretically, precise spectral overlap between the absorption of the acceptor and the Ex/Em of the donor would strengthen the IFE efficiency and ultimately enhance the sensitivity of immunoassays . Meanwhile, as typical reporter NPs, the particle size of AuNPs also matters for fluorescence quenching ability and sensitivity in the LFIA. , For instance, AuNPs smaller than 50 nm showed high scattering intensity owing to the Mie extinction coefficient, while large AuNPs could remarkably strengthen the colorimetric signal but suffer from poor colloidal stability .…”

Section: Resultsmentioning

confidence: 99%

“…Theoretically, the fluorescence-based LFIA with the “turn on” mode has higher sensitivity than that of the “turn off” mode in the competitive-type LFIA . For typically quenching mechanisms, although the inner filter effect (IFE) and fluorescence resonance energy transfer (FRET) both rely on the absorption spectrum of the quencher overlapped with the excitation/emission (Ex/Em) spectrum of the donor, IFE dispensed with the specific distance (while 1–10 nm is needed in FRET) is simplistic, cost-effective, and easier to implement in the LFIA system. Theoretically, the expanded spectral overlap significantly maximizes the IFE quenching efficiency, and quenching emission light is more effective than excitation light .…”

Section: Introductionmentioning

confidence: 99%

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Precise Spectral Overlap-Based Donor–Acceptor Pair for a Sensitive Traffic Light-Typed Bimodal Multiplexed Lateral Flow Immunoassay

Liu,

Shu,

Zhao

et al. 2024

Anal. Chem.

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Bimodal-type multiplexed immunoassays with complementary mode-based correlation analysis are gaining increasing attention for enhancing the practicability of the lateral flow immunoassay (LFIA). Nonetheless, the restriction in visually indistinguishable multitargets induced by a single fluorescent color and difficulty in single acceptor ineffectual fluorescence quenching due to the various spectra of multiple different donors impede the further execution of colorimetric−fluorescence bimodal-type multiplexed LFIAs. Herein, the precise spectral overlap-based donor−acceptor pair construction strategy is proposed by regulating the size of the nanocore, coating it with an appropriate nanoshell, and selecting a suitable fluorescence donor with distinct colors. By in situ coating Prussian blue nanoparticles (PBNPs) on AuNPs with a tunable size and absorption spectrum, the resultant APNPs demonstrate efficient fluorescence quenching ability, higher colloidal stability, remarkable colorimetric intensity, and an enhanced antibody coupling efficiency, all of which facilitate highly sensitive bimodal-type LFIA analysis. Following integration with competitive-type immunoreaction, this precise spectral overlap-supported spatial separation traffic light-typed colorimetric−fluorescence dual-response assay (coined as the STCFD assay) with the limits of detection of 0.013 and 0.152 ng mL −1 for ractopamine and clenbuterol, respectively, was proposed. This work illustrates the superiority of the rational design of a precise spectral overlap-based donor−acceptor pair, hinting at the enormous potential of the STCFD assay in the point-of-care field.

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