Demonstration of Heat Stable and Heat Labile Antigens of Bacteroides Fragilis with the Use of Co-Agglutination and Immunoelectrophoresis Techniques

Rabbit hyperimmune sera against nine Bacteroides fragilis strains were tested in indirect immunofluorescence against homologous and heterologous formalin and heat‐treated bacterial cells. Strain‐specific heat‐stable and heat‐labile antigens were demonstrated as well as many cross‐reactive ones. Besides these findings a striking non‐homogeneous fluorescence was noted, inasmuch as varying numbers of the bacterial cells were stained while others were not. Results of absorption experiments indicated that the reactive antigens were heat‐labile. Non‐homogeneous fluorescence was also demonstrated with sera from patients with clinically significant Bacteroides fragilis infections.

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DEMONSTRATION OF HEAT‐LABILE ANTIGEN(S) OF BACTEROIDES FRAGILIS ASSOCIATED WITH NON‐HOMOGENEOUS IMMUNOFLUORESCENT STAINING

Schwan

Danielsson

Forsum

1982

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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Immune Response to Bacteroides Fragilis in Experimentally Infected Rabbits

Schwan

Danielsson

1983

Scandinavian Journal of Infectious Diseases

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Experimental Bacteroides fragilis infections in rabbits were produced by injecting 10(9)-10(10) organisms into subcutaneously implanted steel-net tissue cages. Four B. fragilis type strains with divergent antigenic set-up were used. Viable counts in the cage contents reached a steady state at 10(7)-10(9) within 2-3 weeks. Bacteria coated with immunoglobulin were demonstrated in the pus. The antibody response in serum, followed with indirect immunofluorescence and detected within 7-10 days, was dominated by IgG; IgM antibodies were also involved but to a lesser degree. Serum antibody levels were not significantly influenced by infecting dose or strain, variations in viable counts or antibiotic therapy. Antibody response was directed against heat-stable as well as heat-labile antigens. Non-homogeneous staining, i.e. positive fluorescence of some of the cells in the preparation with the remaining cells negative, was found with heterologous and homologous cells and was found to be related to heat-labile antigen.

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Demonstration of Heat Stable and Heat Labile Antigens of Bacteroides Fragilis with the Use of Co-Agglutination and Immunoelectrophoresis Techniques

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DEMONSTRATION OF HEAT‐LABILE ANTIGEN(S) OF BACTEROIDES FRAGILIS ASSOCIATED WITH NON‐HOMOGENEOUS IMMUNOFLUORESCENT STAINING

DEMONSTRATION OF HEAT‐LABILE ANTIGEN(S) OF BACTEROIDES FRAGILIS ASSOCIATED WITH NON‐HOMOGENEOUS IMMUNOFLUORESCENT STAINING

Immune Response to Bacteroides Fragilis in Experimentally Infected Rabbits

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