Demonstration of Eosinophil Chemotactic Factor in the Blister Fluid of Patient With Incontinentia Pigmenti

Tsuda, Shingo; Higuchi, M; Ichiki, Mikio; Sasai, Yoichiro

doi:10.1111/j.1346-8138.1985.tb02855.x

Cited by 8 publications

(3 citation statements)

References 22 publications

(12 reference statements)

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“…Nevertheless, the mechanism by which eosinophils accumulate in IP is not yet fully understood. Investigations into the pathomechanism of IP uncovered an unidentified eosinophil chemotactic factor in the blister fluid of patients with IP and also in the extracts of their crusted scales [9,10]. Eotaxin, a CC class chemokine, is a recently identified eosinophil‐selective chemokine [11–16].…”

Section: Introductionmentioning

confidence: 99%

Expression of eotaxin, an eosinophil-selective chemokine, parallels eosinophil accumulation in the vesiculobullous stage of incontinentia pigmenti

Jean-Baptiste

O’Toole

Chen

et al. 2002

Clinical and Experimental Immunology

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SUMMARY Incontinentia pigmenti (IP) is an X‐linked dominant genodermatosis primarily affecting female children. The initial vesiculobullous stage of IP is characterized clinically by inflammatory papules, blisters, and pustules, and histopathologically by acanthosis, keratinocyte necrosis, epidermal spongiosis and massive epidermal eosinophil infiltration. The cause of this multisystem disease is attributed to the mutations of an X‐linked regulatory gene, termed nuclear factor‐κB essential modulator (NEMO). The exact mechanism of epidermal eosinophil accumulation has not yet been determined. We explored the possible role of an eosinophil‐selective, nuclear factor‐κB‐activated chemokine, eotaxin, in the accumulation of eosinophils in the initial stage of the disease. Monoclonal antibody (6H9) specific for human eotaxin strongly labelled the suprabasal epidermis of IP skin, paralleling the upper epidermal accumulation of eosinophils, but did not label the epidermis of normal skin or lesional skin from patients with other inflammatory skin diseases not characterized by prominent eosinophil accumulation, namely dermatitis herpetiformis and selected cases of atopic dermatitis lacking significant numbers of eosinophils. In addition, endothelial cells in lesional skin of IP also exhibited strong expression of eotaxin, which correlated with perivascular and intravascular eosinophil infiltration. We also examined the in vitro effects on epidermally derived eotaxin of several cytokines that were nuclear factor‐κB‐activated and/or known to induce eotaxin expression. In normal human keratinocytes, proinflammatory cytokines either independently (IL‐1α) or synergistically (tumour necrosis factor‐alpha (TNF‐α)/ interferon‐gamma (IFN‐γ) and TNF‐α/IL‐4) up‐regulated eotaxin expression. These studies suggest that release of cytokines during the initial inflammatory stage of IP induces epidermal expression of eotaxin, which may play a role in the epidermal accumulation of eosinophils.

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Section: Introductionmentioning

confidence: 99%

Expression of eotaxin, an eosinophil-selective chemokine, parallels eosinophil accumulation in the vesiculobullous stage of incontinentia pigmenti

Jean-Baptiste

O’Toole

Chen

et al. 2002

Clinical and Experimental Immunology

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“…Although some of these ECFs are thought to be operative at the lesional skin o f dermatoses character ized by eosinophil infiltration, there are only a few papers reporting the determination of these factors in such dermatoses. Low molecular weight ECFA-likc substances were demonstrated in the blisters of bullous pemphigoid [6], incontinentia pigmenti [7], or drug eruption, and in the suction blisters fluids obtained from the lesional skin of atopic derm atitis cases [8],…”

Section: Eosinophil Chemotactic Factor and Se Baceous Glandsmentioning

confidence: 99%

Eosinophilic Pustular Folliculitis

Ofuji¹

1987

Dermatology

160

198

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“…Schmalstieg et al (19) noted the presence of basophils in early vesicular lesions of incontinentia pigmenti and suggested that their presence may be a mechanism of eosinophii recruitment into tissues through histamine and eosinophii chemotactic factor release. Tsuda et al (20) demonstrated the presence of eosinophii chemotactic factor in the blister fluid of patients with incontinentia pigmenti. In 1986 Takematsu et al (21) reported the presence of eosinophii chemotactic activity in extracts of crusted scales from patients whose disease was in the vesiculobullous stage.…”

Section: Discussionmentioning

confidence: 99%

Localization of Eosinophil Granule Major Basic Protein in Incontinentia Pigmenti

Thyresson

Goldberg

Tye

et al. 1991

Pediatric Dermatology

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We report a case of incontinentia pigmenti and demonstrate the deposition and localization of eosinophil major basic protein (MBP) in the vesicular stage of this neurocutaneous syndrome. The initial stage of incontinentia pigmenti is histologically characterized by intraepidermal vesicles associated with eosinophilic spongiotic dermatitis. Pathologic examination of a lesional tissue specimen from our patient demonstrated epidermal necrosis, spongiosis, and vesicle formation. Indirect immunofluorescence with affinity-chromatography purified antibody to human eosinophil granule MBP demonstrated many intact eosinophils within vesicles and scattered throughout the epidermis and dermis, and extracellular deposition of granular MBP in the tissue. The characteristic finding of extensive tissue eosinophilia in incontinentia pigmenti, as well as the new finding of extracellular deposition of an eosinophil granule protein in lesional tissue, suggests the involvement of eosinophils in the pathogenesis of the disease.

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Demonstration of Eosinophil Chemotactic Factor in the Blister Fluid of Patient With Incontinentia Pigmenti

Cited by 8 publications

References 22 publications

Expression of eotaxin, an eosinophil-selective chemokine, parallels eosinophil accumulation in the vesiculobullous stage of incontinentia pigmenti

Expression of eotaxin, an eosinophil-selective chemokine, parallels eosinophil accumulation in the vesiculobullous stage of incontinentia pigmenti

Eosinophilic Pustular Folliculitis

Localization of Eosinophil Granule Major Basic Protein in Incontinentia Pigmenti

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