DEMONSTRATION OF CULTIVABILITY FOR A LOW IONIC STRENGTH WATER<i>HYALELLA AZTECA</i>STRAIN

Walsh, Stephanie; Ng, Christina; Shultz, Carmen; Stuart, Marilyne

doi:10.12943/cnr.2016.00004

Cited by 1 publication

(3 citation statements)

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“…Culturing the Twin Lake strain in the standard artificial medium water formulated for the Canada Centre for Inland Waters strain would not be ideal because it was not representative of conditions natural to Twin Lake H. azteca . Attempts at culturing Twin Lake H. azteca in diluted standard artificial medium yielded low reproduction compared to reproduction observed in the SAM‐5S Canada Centre for Inland Waters culture (Walsh et al ).…”

Section: Introductionmentioning

confidence: 99%

“…It is known to be a cryptic species complex because, although morphologically similar, there are many genetically distinct clades of H. azteca which can vary in size and in sensitivity to toxicants (Leung et al ). Twin Lake and Canada Centre for Inland Waters organisms are clade 1 (Leung et al ; Walsh et al ). The Chalk River Laboratory Toxicity Laboratory cultures and tests with these organisms were in a standard artificial medium (SAM), consisting of 5 salts (SAM‐5S; CaCl 2 , NaHCO 3 , NaBr, KCl, and MgSO 4 ), which is representative of the water qualities found near Lake Ontario, where the culture originated (Borgmann ).…”

Section: Introductionmentioning

confidence: 99%

“…Over the years, attempts have been made to culture the Canada Centre for Inland Waters strain of H. azteca in various diluted standard artificial mediums and water from local water bodies (Ottawa River and Twin Lake waters); however, the Canada Centre for Inland Waters H. azteca did not reproduce in the softer water conditions, and testing was not possible (Walsh et al ).…”

Section: Introductionmentioning

confidence: 99%

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The establishment of a new culture ofHyalella aztecathat would permit toxicity tests to be conducted on low–ionic strength waters

Walsh

Gosselin

Lee

et al. 2019

Enviro Toxic and Chemistry

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The objective of the present study was to establish a culture of Hyalella azteca that could be used for laboratory toxicity testing in low–ionic strength waters with electrical conductivities of <200 μS/cm. A wild strain of H. azteca was collected from Twin Lake, a small seepage lake with an electrical conductivity of 81 ± 27 μS/cm located on the property of Chalk River Laboratories in Chalk River, Canada. To determine the minimum aqueous ion requirements for an optimal culturing medium for the Twin Lake strain, Twin Lake was monitored for water quality and ionic content over 4 yr. Water quality parameters were averaged and used to formulate a medium containing NaHCO3, CaCl2, MgSO4, KCl, NaBr, NaF, and LiCl, with an electrical conductivity of 89 ± 3 μS/cm. By evaluating survival and reproduction, it was concluded that this artificial medium promoted survival and supported reproduction (10 ± 4 neonates/female/wk) of the Twin Lake amphipod. The Twin Lake strain of H. azteca can, therefore, be maintained in laboratory settings, and this allows for toxicity testing to be conducted on low–ionic strength waters. Environ Toxicol Chem 2019;38:585–590. © 2019 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals, Inc. on behalf of SETAC.

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Section: Introductionmentioning

confidence: 99%